Plant-PGPR interaction study of plant growth-promoting diazotrophs Kosakonia radicincitans BA1 and Stenotrophomonas maltophilia COA2 to enhance growth and stress-related gene expression in Saccharum spp.

Singh, Rajesh Kumar; Singh, Pratiksha; Li, Hai-Bi; Guo, Dao-Jun; Song, Qianqian; Yang, Li‐Tao; Malviya, Mukesh Kumar; Song, Xiu–Peng; Li, Yang–Rui

doi:10.1080/17429145.2020.1857857

Cited by 37 publications

(28 citation statements)

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“…Consistent with previous reports, in this study, we observed IAA production by strain S185 in the presence and absence of tryptophan in the medium. Several bacterial strains produce secondary metabolites such as HCN and ammonia, which are helpful in the management of fungal diseases in a variety of plants [ 31 , 49 , 50 , 51 ]. Similarly, the strain S185 showed strong ammonia production as well as potent antifungal activities against Foc , demonstrating the dual role of S185 in controlling FWB.…”

Section: Discussionmentioning

confidence: 99%

“…The extracted compound 1 from S185 was shown as a white powder and directly identified using high-resolution electrospray ionization mass spectrometry (HR-ESI-MS): m/z [M + H] + , 1057.5671 (Figure 6). Based on this information, the molecular formula of this compound was predicted as C 49 13 . All these data were compared with published NMR data and were consistent with the data for iturin A5 [33].…”

Section: Identification Of Compound 1 As Iturin A5mentioning

confidence: 99%

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A Thermotolerant Marine Bacillus amyloliquefaciens S185 Producing Iturin A5 for Antifungal Activity against Fusarium oxysporum f. sp. cubense

Singh

Xie

et al. 2021

Marine Drugs

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Fusarium wilt of banana (also known as Panama disease), is a severe fungal disease caused by soil-borne Fusarium oxysporum f. sp. cubense (Foc). In recent years, biocontrol strategies using antifungal microorganisms from various niches and their related bioactive compounds have been used to prevent and control Panama disease. Here, a thermotolerant marine strain S185 was identified as Bacillus amyloliquefaciens, displaying strong antifungal activity against Foc. The strain S185 possesses multiple plant growth-promoting (PGP) and biocontrol utility properties, such as producing indole acetic acid (IAA) and ammonia, assimilating various carbon sources, tolerating pH of 4 to 9, temperature of 20 to 50 °C, and salt stress of 1 to 5%. Inoculation of S185 colonized the banana plants effectively and was mainly located in leaf and root tissues. To further investigate the antifungal components, compounds were extracted, fractionated, and purified. One compound, inhibiting Foc with minimum inhibitory concentrations (MICs) of 25 μg/disk, was identified as iturin A5 by high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) and nuclear magnetic resonance (NMR). The isolated iturin, A5, resulted in severe morphological changes during spore germination and hyphae growth of Foc. These results specify that B. amyloliquefaciens S185 plays a key role in preventing the Foc pathogen by producing the antifungal compound iturin A5, and possesses potential as a cost-effective and sustainable biocontrol strain for Panama disease in the future. This is the first report of isolation of the antifungal compound iturin A5 from thermotolerant marine B. amyloliquefaciens S185.

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Section: Discussionmentioning

confidence: 99%

Section: Identification Of Compound 1 As Iturin A5mentioning

confidence: 99%

A Thermotolerant Marine Bacillus amyloliquefaciens S185 Producing Iturin A5 for Antifungal Activity against Fusarium oxysporum f. sp. cubense

Singh

Xie

et al. 2021

Marine Drugs

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“…Previously, the PGPB in sugarcane rhizosphere is widely investigated and some potent isolates were obtained ( Lamizadeh et al, 2016 ; Wang Z. et al, 2019 ; Singh et al, 2020 ). However, the information using proteomics to analyze the interaction mechanism between PGPB and sugarcane is limited.…”

Section: Discussionmentioning

confidence: 99%

“…It is effective to use these N-fixing bacteria to improve nitrogen nutrition in plants which is necessary for crop production, especially in sugarcane ( Wei et al, 2014 ; Singh et al, 2017 ). At present, some reports are available on the isolation of N-fixing microorganisms with PGP traits from rhizospheric soil or tissues of sugarcane, such as Klebsiella variicola , Kosakonia sacchari , Streptomyces chartreusis , Pseudomonas spp., Herbaspirillum seropedicae , Stenotrophomonas maltophilia , Kosakonia radicincitans , Bacillus spp., Pantoea dispersa , and Enterobacter asburiae ( Chen et al, 2014 ; Wang Z. et al, 2016 ; Li et al, 2017 ; Antunes et al, 2019 ; Singh et al, 2020 , 2021b ).…”

Section: Introductionmentioning

confidence: 99%

Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5

Guo

Singh

et al. 2021

Front. Plant Sci.

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Plant endophytic bacteria have many vital roles in plant growth promotion (PGP), such as nitrogen (N) fixation and resistance to biotic and abiotic stresses. In this study, the seedlings of sugarcane varieties B8 (requires a low concentration of nitrogen for growth) and GT11 (requires a high concentration of nitrogen for growth) were inoculated with endophytic diazotroph Enterobacter roggenkampii ED5, which exhibits multiple PGP traits, isolated from sugarcane roots. The results showed that the inoculation with E. roggenkampii ED5 promoted the growth of plant significantly in both sugarcane varieties. 15N detection at 60 days post-inoculation proved that the inoculation with strain ED5 increased the total nitrogen concentration in the leaf and root than control in both sugarcane varieties, which was higher in B8. Biochemical parameters and phytohormones in leaf were analyzed at 30 and 60 days after the inoculation. The results showed that the inoculation with E. roggenkampii ED5 improved the activities of superoxide dismutase (SOD), catalase (CAT), NADH-glutamate dehydrogenase (NADH-GDH), glutamine synthetase (GS), and endo-β-1,4-glucanase, and the contents of proline and indole acetic acid (IAA) in leaf, and it was generally more significant in B8 than in GT11. Tandem Mass Tags (TMT) labeling and liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to perform comparative proteomic analysis in the sugarcane leaves at 30 days after inoculation with strain ED5. A total of 27,508 proteins were detected, and 378 differentially expressed proteins (DEPs) were found in the treated sugarcane variety B8 (BE) as compared to control (BC), of which 244 were upregulated and 134 were downregulated. In contrast, a total of 177 DEPs were identified in the treated sugarcane variety GT11 (GE) as compared to control (GC), of which 103 were upregulated and 74 were downregulated. The DEPs were associated with nitrogen metabolism, photosynthesis, starch, sucrose metabolism, response to oxidative stress, hydrolase activity, oxidative phosphorylation, glutathione metabolism, phenylpropanoid metabolic process, and response to stresses in Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database. To the best of our knowledge, this is the first proteomic approach to investigate the molecular basis of the interaction between N-fixing endophytic strain E. roggenkampii ED5 and sugarcane.

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“…Stenotrophomonas species are free-living, Gram-negative nonsporeformers that are commonly found in the soil and plant environments ( 1 ). Stenotrophomonas species can be involved in plant growth promotion (PGP) ( 2 ), although some are yet to be explored. Therefore, genomic elucidation can help predict the diverse genes responsible for bacterial functions in agricultural biotechnology.…”

Section: Announcementmentioning

confidence: 99%

Draft Genome Sequencing of Stenotrophomonas indicatrix BOVIS40 and Stenotrophomonas maltophilia JVB5, Two Strains with Identifiable Genes Involved in Plant Growth Promotion

Babalola

Adeleke

2021

Microbiol Resour Announc

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Here, plant growth-promoting Stenotrophomonas strains isolated from the sunflower root endosphere were studied, yielding molecular insights. Bacterial DNA was sequenced on Illumina’s NextSeq platform. The gene prediction reveals diverse functional genes involved in plant growth promotion from each bacterial genome. The exploration of bacterial resources as bioinoculants is promising for agricultural biotechnology.

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Plant-PGPR interaction study of plant growth-promoting diazotrophs Kosakonia radicincitans BA1 and Stenotrophomonas maltophilia COA2 to enhance growth and stress-related gene expression in Saccharum spp.

Cited by 37 publications

References 114 publications

A Thermotolerant Marine Bacillus amyloliquefaciens S185 Producing Iturin A5 for Antifungal Activity against Fusarium oxysporum f. sp. cubense

A Thermotolerant Marine Bacillus amyloliquefaciens S185 Producing Iturin A5 for Antifungal Activity against Fusarium oxysporum f. sp. cubense

Differential Protein Expression Analysis of Two Sugarcane Varieties in Response to Diazotrophic Plant Growth-Promoting Endophyte Enterobacter roggenkampii ED5

Draft Genome Sequencing of Stenotrophomonas indicatrix BOVIS40 and Stenotrophomonas maltophilia JVB5, Two Strains with Identifiable Genes Involved in Plant Growth Promotion

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