Plant mRNAs move into a fungal pathogen via extracellular vesicles to reduce infection

Wang, Shumei; He, Baoye; Wu, Huaitong; Cai, Qiang; Ramírez-Sánchez, Obed; Abreu-Goodger, Cei; Birch, Paul R.J.; Jin, Hailing

doi:10.1016/j.chom.2023.11.020

Cited by 11 publications

(5 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The process involves tissue infiltration centrifugation to obtain an exosome wash, followed by purification through differential centrifugation, ultrahigh-speed centrifugation, and density gradient centrifugation. When exploring the physiological roles of extracellular vesicles in plants, such as their ability to resist external infections, researchers typically isolate plant-derived extracellular vesicles with higher purity [ 7 , 8 ].…”

Section: Overview: Isolation and Characterization Of Pdnvsmentioning

confidence: 99%

“…These findings reveal the unique functions of PDNVs miRNAs and provide valuable clues for further research into the use of these miRNAs for microbial regulation and therapy. It is worth noting that RNA components other than miRNAs may also be present in PDNVs, especially in the case of PEVs, where it was found that extracellular vesicles secreted by Arabidopsis thaliana cells could deliver sRNAs [ 7 ] and mRNAs [ 8 ] to Staphylococcus , silencing disease-causing genes that play a key role or translationally expressing them in the causative organisms in order to reduce the infection of the plant. As such, there is much to explore in this area.…”

Section: Regulation: Pdnvs Synergistically Regulate Microbiota Throug...mentioning

confidence: 99%

“…PEVs are obtained through the infiltration of plant tissues or isolated from plant cell culture supernatants [ 6 ]. These PEVs play a crucial role in plants by delivering small RNAs (sRNAs) and messenger RNAs (mRNAs) to defend against external infections [ 7 , 8 ]. Conversely, PDNVs are obtained by directly isolating plant tissues using differential centrifugation and density gradient centrifugation methods [ 9 ], which encompass not only extracellular vesicles but also other substances such as vesicle-like structure [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Exploring new avenues of health protection: plant-derived nanovesicles reshape microbial communities

Chen,

He,

Zhang

et al. 2024

J Nanobiotechnol

View full text Add to dashboard Cite

Symbiotic microbial communities are crucial for human health, and dysbiosis is associated with various diseases. Plant-derived nanovesicles (PDNVs) have a lipid bilayer structure and contain lipids, metabolites, proteins, and RNA. They offer unique advantages in regulating microbial community homeostasis and treating diseases related to dysbiosis compared to traditional drugs. On the one hand, lipids on PDNVs serve as the primary substances that mediate specific recognition and uptake by bacteria. On the other hand, due to the multifactorial nature of PDNVs, they have the potential to enhance growth and survival of beneficial bacterial while simultaneously reducing the pathogenicity of harmful bacteria. In addition, PDNVs have the capacity to modulate bacterial metabolism, thus facilitating the establishment of a harmonious microbial equilibrium and promoting stability within the microbiota. These remarkable attributes make PDNVs a promising therapeutic approach for various conditions, including periodontitis, inflammatory bowel disease, and skin infection diseases. However, challenges such as consistency, isolation methods, and storage need to be addressed before clinical application. This review aims to explore the value of PDNVs in regulating microbial community homeostasis and provide recommendations for their use as novel therapeutic agents for health protection. Graphical Abstract

show abstract

Section: Overview: Isolation and Characterization Of Pdnvsmentioning

confidence: 99%

Section: Regulation: Pdnvs Synergistically Regulate Microbiota Throug...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Exploring new avenues of health protection: plant-derived nanovesicles reshape microbial communities

Chen,

He,

Zhang

et al. 2024

J Nanobiotechnol

View full text Add to dashboard Cite

show abstract

“…Despite the great focus on the ways that SA-derived EVs can affect the host to benefit pathogen establishment, one might wonder whether host EVs could not affect S. aureus behavior. Recently, Wang et al [ 66 ] demonstrated that the model plant Arabidopis thaliana uses its EVs to deliver mRNAs to the fungal pathogen Botrytis cinerea , attenuating its virulence. Although this idea remains largely unappreciated in the AD field, it opens new venues of investigation for exploring host— S. aureus interaction.…”

Section: Future Research Questionsmentioning

confidence: 99%

State of the Art on the Role of Staphylococcus aureus Extracellular Vesicles in the Pathogenesis of Atopic Dermatitis

Torrealba,

Yoshikawa,

Aoki

et al. 2024

Microorganisms

View full text Add to dashboard Cite

Atopic dermatitis (AD) is a chronic and relapsing inflammatory cutaneous disease. The role of host defense and microbial virulence factors in Staphylococcus aureus skin colonization, infection, and inflammation perpetuation in AD remains an area of current research focus. Extracellular vesicles (EV) mediate cell-to-cell communication by transporting and delivering bioactive molecules, such as nucleic acids, proteins, and enzymes, to recipient cells. Staphylococcus aureus spontaneously secretes extracellular vesicles (SA-derived EVs), which spread throughout the skin layers. Previous research has shown that SA-derived EVs from AD patients can trigger cytokine secretion in keratinocytes, shape the recruitment of neutrophils and monocytes, and induce inflammatory AD-type lesions in mouse models, in addition to their role as exogenous worsening factors for the disease. In this review article, we aim to examine the role of SA-derived EVs in AD physiopathology and its progression, highlighting the recent research in the field and exploring the potential crosstalk between the host and the microbiota.

show abstract

“…Proteomic analyses of purified EV revealed a cocktail of defense-related compounds, lipids, and RNA ( Rutter and Innes, 2017 ). The EV are secreted to the extracellular space and taken up by fungal pathogens, resulting in a decrease in virulence ( Wang et al, 2024 ). Conversely, pathogenic fungi also release EV, which are internalized via clathrin-mediated endocytosed by plant cells, supporting that EV allow communication between plants and pathogens ( He et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Logistics of defense: The contribution of endomembranes to plant innate immunity

Bhandari,

Brandizzi

2024

Journal of Cell Biology

View full text Add to dashboard Cite

Phytopathogens cause plant diseases that threaten food security. Unlike mammals, plants lack an adaptive immune system and rely on their innate immune system to recognize and respond to pathogens. Plant response to a pathogen attack requires precise coordination of intracellular traffic and signaling. Spatial and/or temporal defects in coordinating signals and cargo can lead to detrimental effects on cell development. The role of intracellular traffic comes into a critical focus when the cell sustains biotic stress. In this review, we discuss the current understanding of the post-immune activation logistics of plant defense. Specifically, we focus on packaging and shipping of defense-related cargo, rerouting of intracellular traffic, the players enabling defense-related traffic, and pathogen-mediated subversion of these pathways. We highlight the roles of the cytoskeleton, cytoskeleton–organelle bridging proteins, and secretory vesicles in maintaining pathways of exocytic defense, acting as sentinels during pathogen attack, and the necessary elements for building the cell wall as a barrier to pathogens. We also identify points of convergence between mammalian and plant trafficking pathways during defense and highlight plant unique responses to illustrate evolutionary adaptations that plants have undergone to resist biotic stress.

show abstract

Plant mRNAs move into a fungal pathogen via extracellular vesicles to reduce infection

Cited by 11 publications

References 71 publications

Exploring new avenues of health protection: plant-derived nanovesicles reshape microbial communities

Exploring new avenues of health protection: plant-derived nanovesicles reshape microbial communities

State of the Art on the Role of Staphylococcus aureus Extracellular Vesicles in the Pathogenesis of Atopic Dermatitis

Logistics of defense: The contribution of endomembranes to plant innate immunity

Contact Info

Product

Resources

About