Plant Molecular Farming: Opportunities and Challenges

Basaran, Pervin; Rodríguez‐Cerezo, Emilio

doi:10.1080/07388550802046624

Cited by 108 publications

(51 citation statements)

References 108 publications

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“…These systems have emerged as promising hosts for AMP production because of their advantages over the currently applied expression systems based on bacteria or yeast [20,21]. Plants could successfully perform appropriate glycosylation, folding and disulphide bond formation of recombinant AMPs that are often essential for their biological activity [18].…”

Section: Introductionmentioning

confidence: 99%

Recombinant expression of LFchimera antimicrobial peptide in a plant-based expression system and its antimicrobial activity against clinical and phytopathogenic bacteria

Chahardoli

Fazeli

Niazi‎

et al. 2018

Biotechnology & Biotechnological Equipment

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Antimicrobial peptides (AMPs) with their broad and selective antimicrobial activity and lowered risk of resistance development in microbial populations are promining as a new alternative candidate to conventional antibiotics. Plant-based expression systems can be employed as cost-effective and high scale-up capacity production hosts for recombinant expression of AMPs. LFchimera is a chimerical peptide containing LFcin and LFampin antimicrobial peptides of bovine lactoferrin, and it has stronger bactericidal activity. Here, the LFchimera sequence was codon-optimized for tobacco and fused with endoplasmic reticulum retention signals along with a CaMV 35S promoter and then transferred by agrobacterium-mediated transformation. The integration and expression of the transgene in tobacco plants were confirmed by polymerase chain reaction (PCR) and RT-PCR, respectively. Recombinant production of the peptide was confirmed by sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE), and peptide functional activity was confirmed by the antibacterial evaluation of total protein extracts against various clinical and phytopathogenic bacteria. Finally, LFchimera peptide was partially purified using an affinity column, and the antibacterial activity was shown. Taken together, these results confirmed that tobacco can be utilized for the recombinant production of antimicrobial peptides such as LFchimera.

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Section: Introductionmentioning

confidence: 99%

Recombinant expression of LFchimera antimicrobial peptide in a plant-based expression system and its antimicrobial activity against clinical and phytopathogenic bacteria

Chahardoli

Fazeli

Niazi‎

et al. 2018

Biotechnology & Biotechnological Equipment

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“…Because of the longer storability of rice seeds, recombinant seed banks could be generated, providing an economically feasible, timely scale-up of therapeutics in response to changing market demands. It is estimated that using achieved yields, enough hepatitis B-antigen to vaccinate all the infants worldwide could be produced on approximately 200 acres of land (Basaran and Rodriguez-Cerezo 2008). There are multiple target molecules that have been successfully produced in rice seeds in academic laboratories and on a commercial scale (http://www.invitria.com).…”

Section: Versatility Of a Rice Seed-based Expression Systemmentioning

confidence: 99%

Strategies to Increase Heterologous Protein Expression in Rice Grains

Nandi

Khush

2015

Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

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“…planta-pharma, plant bioreactors, plant biofactory, 그리고 pharmaceutical gardening 등 많은 용어들이 비슷한 의미로 혼용하여 사용하고 있다 (Basaran and Rodriguez-Cerezo 2008 (Mason et al 1998;Kang et al 2004), 유전자의 안정성에 영향을 미 치는 요소 제거 (De Jaeger et al 2002;Ibrahim et al 2001;Outchkourov et al 2003) (Barta et al 1986;De Zoeten et al 1989;Sijmons et al 1990), 1989년에는 담배의 잎에서 항체를 발현을 시킴으로서 식물에서 복잡 한 형태의 치료용 동물단백질을 생산할 수 있다는 가능성 이 제시되었다 (Hiatt et al 1989 (Poirier et al 1992).…”

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Current status in molecular farming

Kim¹,

Yang

2010

Journal of Plant Biotechnology

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Molecular farming is production of pharmaceutically and industrially important proteins in plants. Plants and plant cell culture systems have been used as bio-factory to produce recombinant proteins such as monoclonal antibodies, enzymes, vaccines, hormones, interleukins, commercial enzymes and etc. The terms molecular farming, biofarming, molecular pharming, phytomanufacturing, recombinant or plant-made industrials, planta-pharma, plant bioreactors, plant biofactory, and pharmaceutical gardening are used interchangeably. Molecular farming can provide safe and inexpensive pharmaceutical proteins as well as commercial ones. In spite of several advantages of molecular farming such as safety and inexpensive cost, there are also a couple of drawbacks in the existing technology. One of them is low expression level of target gene in plants, which has been improved by optimizing gene-based codon usage, screening of strong promoters, expression of transcription factors, subcellular targeting of target proteins, chloroplast transformation, and transient expression using viral expression system (magnifection). Some plant-based commercial proteins have already been in markets and more than twenty plant-based pharmaceuticals have been in clinical trials, from that we can expect that several plant-based pharmaceutical proteins will be seen in the markets in the near future.

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Plant Molecular Farming: Opportunities and Challenges

Cited by 108 publications

References 108 publications

Recombinant expression of LFchimera antimicrobial peptide in a plant-based expression system and its antimicrobial activity against clinical and phytopathogenic bacteria

Recombinant expression of LFchimera antimicrobial peptide in a plant-based expression system and its antimicrobial activity against clinical and phytopathogenic bacteria

Strategies to Increase Heterologous Protein Expression in Rice Grains

Current status in molecular farming

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