Plant microProteins and miPEPs: Small molecules with much bigger roles

Gautam, Himanshi; Sharma, Ashish; Trivedi, Prabodh Kumar

doi:10.1016/j.plantsci.2022.111519

Cited by 12 publications

(9 citation statements)

References 58 publications

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“…The primary transcript of miRNA has been found to encode microRNA encoded peptides (miPEPs), which enhance the transcription of their corresponding miRNAs (Lauressegues et al, 2015, Gautam et al, 2023). To identify functional domains within miPEPs or their potential as full-length peptides, we focused on miPEP858, known to modulate the expression of miR858 and its target genes.…”

Section: Identification Of Functional Truncated Peptidesmentioning

confidence: 99%

miR858a-encoded peptide, miPEP858a, interacts with miR858a promoter and requires C-terminus for the associated functions

Gautam,

Sharma,

Anyatama

et al. 2024

Preprint

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MicroRNAs (miRNAs) play a crucial role in regulating gene expression as processed products of primary miRNAs (pri-miRNAs). Recent studies suggest that certain pri-miRNAs encode miRNA-encoded Peptides (miPEPs), influencing miRNA function. Despite this emerging insight, molecular mechanisms and the specific domain or essential amino acid residues required for the functionality of these miPEPs have yet to be explored. Here, we report that the pri-miR858a-encoded peptide, miPEP858a, interacts with the promoter region of the MIR858 gene and its C-terminal region consisting of 14 amino acid residues is indispensable for its functionality. DNA-protein interaction through Yeast-one-hybrid and ChIP-qPCR analysis revealed that miPEP858 interacts with specific region in the MIR858 promoter. An exogenous application of a truncated peptide, the C-terminal region, resulted in enhanced expression and related phenotypic effects, similar to complete miPEP858. Furthermore, the C-terminal region of miPEP858a complemented mutant plants lacking miPEP858, highlighting their regulatory role in miR858a expression and associated target genes, impacting flavonoid biosynthesis and plant development. These findings suggest that full-length miPEP858a may not be essential for its functionality. Identifying functional domains of other peptides offers promise, potentially reducing synthesis costs for application in crop plants and avoiding laborious biotechnological approaches to enhance agronomic traits.

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Section: Identification Of Functional Truncated Peptidesmentioning

confidence: 99%

miR858a-encoded peptide, miPEP858a, interacts with miR858a promoter and requires C-terminus for the associated functions

Gautam,

Sharma,

Anyatama

et al. 2024

Preprint

Self Cite

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“…A novel frontier in crop improvement is the topical delivery of biomolecules, including RNAs and peptides, as another transgenefree alternative (Gautam et al, 2023;Landry & Mitter, 2019).…”

Section: Perspectives and Challenges For Improving Plant Resilience T...mentioning

confidence: 99%

“…A novel frontier in crop improvement is the topical delivery of biomolecules, including RNAs and peptides, as another transgene‐free alternative (Gautam et al, 2023; Landry & Mitter, 2019). Although yet to be explored in cold responses, the exogenous application of biomolecules offers the great advantage of preserving the variety of locally adapted landraces.…”

Section: Perspectives and Challenges For Improving Plant Resilience T...mentioning

confidence: 99%

Is winter coming? Impact of the changing climate on plant responses to cold temperature

Larran

Pajoro

Qüesta

2023

Plant Cell & Environment

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Climate change is causing alterations in annual temperature regimes worldwide. Important aspects of this include the reduction of winter chilling temperatures as well as the occurrence of unpredicted frosts, both significantly affecting plant growth and yields. Recent studies advanced the knowledge of the mechanisms underlying cold responses and tolerance in the model plant Arabidopsis thaliana. However, how these cold‐responsive pathways will readjust to ongoing seasonal temperature variation caused by global warming remains an open question. In this review, we highlight the plant developmental programmes that depend on cold temperature. We focus on the molecular mechanisms that plants have evolved to adjust their development and stress responses upon exposure to cold. Covering both genetic and epigenetic aspects, we present the latest insights into how alternative splicing, noncoding RNAs and the formation of biomolecular condensates play key roles in the regulation of cold responses. We conclude by commenting on attractive targets to accelerate the breeding of increased cold tolerance, bringing up biotechnological tools that might assist in overcoming current limitations. Our aim is to guide the reflection on the current agricultural challenges imposed by a changing climate and to provide useful information for improving plant resilience to unpredictable cold regimes.

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“…Plant peptides are synthesized from precursor proteins or small open reading frames (sORFs) embedded within transcripts. These sORFs comprise 100 codons or less and lie either in the 5′‐leader sequence of a messenger RNA or are embedded within the primary transcripts of microRNAs (miRNAs) or are present within transcripts that code for short proteins of length <100 amino acids (Gautam et al, 2023; Hanada et al, 2013; Kumar et al, 2023; Lauressergues et al, 2015; Lauressergues et al, 2022; Ormancey et al, 2023; Sharma et al, 2020; Von Arnim et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Small but mighty: Peptides regulating abiotic stress responses in plants

Datta,

Kumar,

Sinha

et al. 2024

Plant Cell & Environment

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Throughout evolution, plants have developed strategies to confront and alleviate the detrimental impacts of abiotic stresses on their growth and development. The combat strategies involve intricate molecular networks and a spectrum of early and late stress‐responsive pathways. Plant peptides, consisting of fewer than 100 amino acid residues, are at the forefront of these responses, serving as pivotal signalling molecules. These peptides, with roles similar to phytohormones, intricately regulate plant growth, development and facilitate essential cell‐to‐cell communications. Numerous studies underscore the significant role of these small peptides in coordinating diverse signalling events triggered by environmental challenges. Originating from the proteolytic processing of larger protein precursors or directly translated from small open reading frames, including microRNA (miRNA) encoded peptides from primary miRNA, these peptides exert their biological functions through binding with membrane‐embedded receptor‐like kinases. This interaction initiates downstream cellular signalling cascades, often involving major phytohormones or reactive oxygen species‐mediated mechanisms. Despite these advances, the precise modes of action for numerous other small peptides remain to be fully elucidated. In this review, we delve into the dynamics of stress physiology, mainly focusing on the roles of major small signalling peptides, shedding light on their significance in the face of changing environmental conditions.

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Plant microProteins and miPEPs: Small molecules with much bigger roles

Cited by 12 publications

References 58 publications

miR858a-encoded peptide, miPEP858a, interacts with miR858a promoter and requires C-terminus for the associated functions

miR858a-encoded peptide, miPEP858a, interacts with miR858a promoter and requires C-terminus for the associated functions

Is winter coming? Impact of the changing climate on plant responses to cold temperature

Small but mighty: Peptides regulating abiotic stress responses in plants

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