Plant membrane biophysics in development and senescence

Leshem, Y.

doi:10.1007/978-94-017-2683-2_7

Cited by 30 publications

(45 citation statements)

References 54 publications

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“…Galactolipid/phospholipid ratio, indicates the abundance of chloroplast membranes in relation to extra-chloroplastic ones (Douce and Joyard, 1980). MGDG/DGDG ratios related to preservation of membrane integrity due to stable DGDG bilayer configuration (Leshem, 1992;TorresFranklin et al, 2007). The fatty acid composition of the lipid classes, in particular the amount of C18:3, strongly contributes to the degree of unsaturation, therefore playing a role in the fluidity of the lipid matrix (Webb and Green, 1991;Harwood, 1998).…”

Section: Resultsmentioning

confidence: 99%

“…Cell membranes are major targets of environmental stresses (Leshem, 1992) and in vitro screening tests for membrane permeability may contribute for genotypes selection under different stress conditions. Electrolyte leakage test has been previously used to evaluate membrane tolerance to dehydration (Vasquez-Tello et al, 1990;Matos et al, 2010;Scotti-Campos et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Membrane lipids play an essential role in cells metabolism, maintaining cell integrity and ensuring the activity of membrane-bound proteins (Leshem, 1992). Under stress conditions, they undergo modifications which either enable them to acclimate to the new environment or lead to irreversible injury and cell death (Kuiper, 1985;Harwood, 1998).Qualitative and quantitative changes of membrane lipids have been involved in adaptation to many abiotic stresses, such as cold (Partelli et al, 2011;Scotti-Campos et al, 2014a), heat (Scotti-Campos et al, 2014b), aluminium toxicity (Huynh et al, 2012) and drought Matos et al, 2010;Scotti-Campos et al, 2013), among others.…”

Section: Introductionmentioning

confidence: 99%

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Correlation between total lipids, linolenic acid and membrane injury under PEG-induced dehydration in leaves of Vigna genotypes differing in drought resistance

Campos

Pham-Thi

2016

Emir. J. Food Agric

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Correlation between total lipids, linolenic acid and membrane injury under PEG-induced dehydration in leaves of Vigna genotypes differing in drought resistance

Campos

Pham-Thi

2016

Emir. J. Food Agric

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“…Age-induced loss of membrane integrity is well established for potato (Knowles and Knowles, 1989). Since H+-ATPases consume 25 to 50% of the ATP content of cells (Leshem et al, 1992) and function in maintaining electrochemical gradients and ionic balance, the leaky cell membranes of older tubers likely constitute a substantial sink for ATP, which, in the absence of increased oxidative phosphorylation (respiration), would effect reduced AEC. To link our previous results of age-enhanced membrane permeability with increased ATP catabolism (energy expenditure), a procedure for isolating PMs from tubers was developed, and the effects of age and sprouting on H+-ATPase activities were compared.…”

Section: Discussionmentioning

confidence: 99%

Oxidative Stress Results in Increased Sinks for Metabolic Energy during Aging and Sprouting of Potato Seed-Tubers

Kumar

Knowles

1996

Plant Physiology

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Glutathione-mediated free-radical-scavenging and plasma membrane ATPase activities increase as sinks for metabolic energy with advancing tuber age. Plasma membrane ATPase activity from 19-month-old tubers was 77% higher than that from 7-month-old tubers throughout sprouting. The higher activity was not attended by an increase in the amount of ATPase per unit plasma membrane protein.Concentrations of oxidized (CSSC) and reduced glutathione more than doubled as tuber age advanced from 6 to 30 months, but the proportion of GSSG to total glutathione remained constant with age. l h e activity of glutathione transferase, an enzyme that catabolizes lipidhydroperoxides, increased by 44 and 205% on a fresh weight and protein basis, respectively, as tubers aged from 6 to 30 months. Glutathione reductase activity also increased with advancing age, by 90% on a fresh weight basis and 305% on a protein basis. Older tubers had more glutathione reductase per unit of soluble and mitochondrial protein. The age-induced increase in cytosolic glutathione transferase activity was likely due to increased availability of lipid-hydroperoxides and/or a positive effector. Synthesis of glutathione requires ATP, and the increased reduction of CSSC resulting from catalysis of lipidhydroperoxides is NADPH-dependent. Thus, increased plasma membrane ATPase and glutathione-mediated free-radical-scavenging activities likely constitute substantial sinks for ATP in older tubers prior to and during sprouting. lncreased oxidative stress and loss in membrane integrity are central features of aging that undoubtedly contribute to the enhanced respiration of sprouting older tubers.Potato (Solanum tuberosum L.) seed-tubers provide a model system for studies of the metabolic processes associated with aging of plant tissues in general and vegetative propagules in particular. Russet Burbank seed-tubers maintain their viability for about 3 years in cold storage (4"C, 95% RH); however, vigor and growth potential of tuber meristems depend on age. As tubers advance in age beyond about 7 months, apical dominance and plant vigor gradually decline, resulting in the production of many shoots per tuber, each with low specific leaf area and sparse root systems (Mikitzel and Knowles, 1990; Kumar and Knowles, 1993a). Paradoxically, older tubers have substantially higher rates of fully coupled, Cyt-mediated respiration than younger tubers at similar stages of sprout development (Kumar and Knowles, 1996). This greater ATP production by sprouting older tubers is required to '

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“…However, if this enzyme is, in some way, involved in some of these phenomena, such as senescence or defense mechanisms, one would expect that it is at tached to membranes [8]. Indeed, lipolytic enzymes liber ate polyunsaturated fatty acids that trigger lipoxygenase activity and, subsequently, other enzymic and nonenzymic reactions, leading to membrane deterioration and disassem bly [35,36]. The acid LOX shown in soybean membrane preparation can better accomplish this role, since it is plasma membrane-bound.…”

Section: Discussionmentioning

confidence: 99%