Plant-mediated gene silencing restricts growth of the potato late blight pathogen Phytophthora infestans

Jahan, Sultana Nilufar; Åsman, Anna K. M.; Corcoran, Pádraic; Fogelqvist, Johan; Vetukuri, Ramesh R.; Dixelius, Christina

doi:10.1093/jxb/erv094

Cited by 125 publications

(84 citation statements)

References 54 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Low copy numbers of sRNAs in peanut has been reported previously [36,37]; where, similar to our study, the majority of the peanut miRNAs had only one copy. Low copy numbers for transgene-derived siRNAs has been reported previously as well: Jahan et al [50] found few and low copy numbers of transgene-derived siRNAs in transgenic potato expressing a hairpin RNA construct targeting the G protein ß-subunit (PiGB1) of Phytophthora infestans, which is an essential gene involved in the pathogenicity of this pathogen. Their transgenic line had reduced DNA content and growth of the pathogen, had less disease, and had reduced transcript levels of the PiGB1 gene compared to the wildtype, nevertheless, the Illumina sRNA sequencing of the line expressing the hairpin PiGB1 revealed only nine sRNAs that mapped to the PiGB1 gene, in low abundance.…”

Section: Discussionmentioning

confidence: 66%

Characterization of small RNA populations in non-transgenic and aflatoxin-reducing-transformed peanut

et al. 2017

View full text Add to dashboard Cite

Power, Imana L.; Dang, Phat M.; Sobolev, Victor S.; Orner, Valerie; Powell, Joseph L.; Lamb, Marshall C.; and Arias, Renée S., "Characterization of small RNA populations in non-transgenic andaflatoxin-reducing-transformed peanut" (2017 t r a c tAflatoxin contamination is a major constraint in food production worldwide. In peanut (Arachis hypogaea L.), these toxic and carcinogenic aflatoxins are mainly produced by Aspergillus flavus Link and A. parasiticus Speare. The use of RNA interference (RNAi) is a promising method to reduce or prevent the accumulation of aflatoxin in peanut seed. In this study, we performed high-throughput sequencing of small RNA populations in a control line and in two transformed peanut lines that expressed an inverted repeat targeting five genes involved in the aflatoxin-biosynthesis pathway and that showed up to 100% less aflatoxin B 1 than the controls. The objective was to determine the putative involvement of the small RNA populations in aflatoxin reduction. In total, 41 known microRNA (miRNA) families and many novel miRNAs were identified. Among those, 89 known and 10 novel miRNAs were differentially expressed in the transformed lines. We furthermore found two small interfering RNAs derived from the inverted repeat, and 39 sRNAs that mapped without mismatches to the genome of A. flavus and were present only in the transformed lines. This information will increase our understanding of the effectiveness of RNAi and enable the possible improvement of the RNAi technology for the control of aflatoxins.

show abstract

Section: Discussionmentioning

confidence: 66%

Characterization of small RNA populations in non-transgenic and aflatoxin-reducing-transformed peanut

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The host-induced gene silencing (HIGS) has emerged in application to control plant pathogens, including fungi and oomycetes (Nowara et al 2010; Tinoco et al 2010; Jahan et al 2015; Zhang et al 2016a). Recently, we generated transgenic cotton plants expressing an RNAi construct targeting V. dahliae hydrophobin gene (designed as VdH1i ), and demonstrated the efficiency and effectiveness of producing VdH1i -derived siRNAs in transgenic cotton plants to confer resistance against V. dahliae infection (Zhang et al 2016a).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots

et al. 2018

View full text Add to dashboard Cite

Small RNAs (sRNAs, including small interfering RNAs [siRNAs] and micro RNAs [miRNAs]) are key mediators of RNA silencing (or RNA interference), which play important roles in plant development and response to biotic and abiotic stimulation. Verticillium wilt is a plant vascular disease caused by the soil-borne fungal pathogens, such as Verticillium dahliae. We previously reported that V. dahliae infection increased two plant endogenous miRNAs that were exported to fungal cell to silence virulence genes. To investigate plant sRNAs in genome-wide response to V. dahliae infection, in this study, we constructed two sRNA libraries from Arabidopsis roots with and without V. dahliae infection, respectively. In total, 31 conserved miRNAs were found to be differentially expressed during the early stage of infection with V. dahliae using sRNA sequencing. Among these, the expression levels of miR160, miR164, miR166, miR167, miR390 and miR156h were confirmed by northern blot. Reverse transcription quantitative real time polymerase chain reaction results showed that the induction of miRNAs (miR160, miR164, miR166 and miR167) upon V. dahliae infection downregulated the expression of their targeted genes (ARF10, NAC1, PHV and ARF6), respectively. In addition, we identified specific phased siRNAs generated from distinct regions of two libraries. Profiling of these miRNAs and sRNAs lay the foundation for further understanding and utilising the host-induced gene silencing strategy to control plant vascular pathogens.

show abstract

“…Host induced gene silencing (HIGS) is a relatively new approach for controlling plant pathogens that relies on RNA interference to target the expression of essential pathogen genes. This strategy has been used to target a wide range of pathogen types including insects (reviews by Baum et al 2007;Huvenne and Smagghe 2010), nematodes (reviewed by Huang et al 2006;Yadav et al 2006;Fairbairn et al 2007;Sindhu et al 2009), fungi (Nowara et al 2010;Tinoco et al 2010;Yin et al 2011;Zhang et al 2012;Koch et al 2013;Panwar et al 2013;Pliego et al 2013;Ghag et al 2014), parasitic weeds (Tomilov et al 2008), and oomycetes (Govindarajulu et al 2014;VegaArreguin et al 2014;Jahan et al 2015). Pathogen effectors essential for virulence or Bhousekeeping^genes necessary for normal pathogen growth are typically the targets for HIGS.…”

Section: Resistance To Biotic and Abiotic Stressesmentioning

confidence: 99%

“…The siRNAs subsequently move into the pathogen during infection to silence the target genes. In the only HIGS results using potato thus far, targeting of the P. infestans gene hp-PiGPB1, which encodes a protein important in pathogenicity, resulted in reduced sporangia formation and disease progression in transgenic plants (Jahan et al 2015). Not only does HIGS provide an experimental tool for determining the significance of specific genes in pathogen virulence, it also presents a new way to control plant diseases without having to identify and clone R genes from the host.…”

Section: Resistance To Biotic and Abiotic Stressesmentioning

confidence: 99%

Biotech Potatoes in the 21st Century: 20 Years Since the First Biotech Potato

et al. 2015

View full text Add to dashboard Cite

Potato is the world's most important vegetable crop, with nearly 400 million tons produced worldwide every year, lending to stability in food supply and socioeconomic impact. In general, potato is an intensively managed crop, requiring irrigation, fertilization, and frequent pesticide applications in order to obtain the highest yields possible. Important traits are easy to find in wild relatives of potato, but their introduction using traditional breeding can take 15-20 years. This is due to sexual incompatibility between some wild and cultivated species, a desire to remove undesirable wild species traits from adapted germplasm, and difficulty in identifying broadly applicable molecular markers. Fortunately, potato is amenable to propagation via tissue culture and it is relatively easy to introduce new traits using currently available biotech transformation techniques. For these reasons, potato is arguably the crop that can benefit most by modern biotechnology. The benefits of biotech potato, such as limited gene flow to conventionally grown crops and weedy relatives, the opportunity for significant productivity and nutritional quality gains, and reductions in production cost and environmental impact, have the potential to influence the marketability of newly developed varieties. In this review we will discuss current and past efforts to develop biotech potato varieties, traits that could be impacted, and the potential effects that biotech potato could have on the industry.Resumen La papa es el cultivo hortícola más importante en el mundo, con cerca de 400 millones de toneladas producidas a nivel mundial anualmente, acreditando la estabilidad en el suministro de alimentos e impacto socioeconómico. En general, la papa es un cultivo manejado intensivamente, que requiere riego, fertilización y aplicaciones frecuentes de plaguicidas para obtener los más altos rendimientos posibles. Los caracteres importantes son fáciles de encontrar en parientes silvestres de la papa, pero su introducción usando el mejoramiento tradicional puede llevar de 15 a 20 años. Esto es debido a la incompatibilidad sexual entre algunas especies silvestres y cultivadas, el deseo para eliminar características indeseables de las especies silvestres del germoplasma adaptado, y la dificultad en la identificación de marcadores moleculares aplicables ampliamente. Afortunadamente, la papa es receptiva a la propagación por cultivo de tejidos y es relativamente fácil la introducción de nuevos caracteres usando técnicas biotecnológicas de transformación actualmente disponibles. Por estas razones, la papa es probablemente el cultivo que se puede beneficiar mayormente por la biotecnología moderna. Los beneficios de la papa biotecnológica, como el flujo genético limitado a cultivos que se siembran convencionalmente y a los parientes como malezas, la oportunidad para productividad significativa y logros en calidad nutricional, y las reducciones en los costos de producción e impacto ambiental, tienen el potencial para influenciar la comercialización...

show abstract

Plant-mediated gene silencing restricts growth of the potato late blight pathogen Phytophthora infestans

Abstract: HighlightA host-induced gene-silencing strategy for controlling potato late blight is presented, a plant disease that conventionally requires regular application of fungicides at high rates.

Cited by 125 publications

References 54 publications

Characterization of small RNA populations in non-transgenic and aflatoxin-reducing-transformed peanut

Characterization of small RNA populations in non-transgenic and aflatoxin-reducing-transformed peanut

Genome-wide profiling of sRNAs in the Verticillium dahliae-infected Arabidopsis roots

Biotech Potatoes in the 21st Century: 20 Years Since the First Biotech Potato

Contact Info

Product

Resources

About