Plant Lipoxygenases and Their Role in Plant Physiology

Kotapati, Kasi Viswanath; Panditi, Varakumar; Pamuru, Ramachandra Reddy; Basha, Shiak Jeelan; Mehta, Sahil; Rao, Ampasala Dinakara

doi:10.1007/s12374-020-09241-x

Cited by 133 publications

(98 citation statements)

References 105 publications

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“…Through a phylogenetic analysis of the LOX gene family in watermelon and other plant species, LOX family members can be divided into the categories of 9-LOXs and 13-LOXs (Figure 1), which is in accordance with the classification of LOXs in previous reports [2,7,13]. All the watermelon 13-LOX members contain the F residue at the active site ( Figure S2), which is indicative of the 13-LOX activity of LOX enzymes [35,36].…”

Section: Discussionsupporting

confidence: 76%

“…Our previous reports have shown that other oxylipin pathway genes, such as ClAOCs, ClAOS and ClHPLs, are also regulated in response to JA, SA, and ET, which may play important roles in watermelon defense against root-knot nematode (RKN) infection [6,30]. The roles of LOXs in the defense against pathogens and pests might be associated with the synthesis of a number of compounds from the oxylipin pathway [1,7]. For example, OsLOX1 could lead to the production of more JA, (Z)-3-hexenal, and colneleic acid after brown plant hopper (BPH) feeding, and transgenic rice plants with enhanced OsLOX1 expression were more resistant to BPH attack [49].…”

Section: Discussionmentioning

confidence: 99%

“…They can catalyze the oxidation of polyunsaturated fatty acids (PUFAs), such as linoleic acid (LA) and α-linolenic acid (α-LeA), into unsaturated fatty acid hydroperoxides [2,5]. In plants, LOX-mediated peroxidation of PUFAs undergoes a series of secondary reactions involving several multigene enzyme families, such as Allene oxide synthase (AOS), hydroperoxide lyase (HPL), divinyl ether synthase (DES), and allene oxide cyclase (AOC), and finally produces a large number of biologically active compounds such as jasmonic acid (JA) and its related chemical compounds [1,6,7].…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive Genomic Characterization and Expression Analysis of the Lipoxygenase Gene Family in Watermelon under Hormonal Treatments

Liu

Zhou

et al. 2020

Agriculture

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Lipoxygenases (LOXs) are non-haem iron-containing dioxygenases and play vital roles in a variety of plant biological processes. Here, we first carried out the genome-wide identification of LOX genes in watermelon. A total of 16 LOX genes were identified, which could be classified into two categories according to phylogenetic analysis: the 9-LOXs (ClLOX1–4, 12, and 15) and 13-LOXs (ClLOX5–11, 13, 14, and 16). Furthermore, the protein structures, intrachromosomal distributions, and gene structures were thoroughly analyzed. Cis-element analysis of the promoter regions indicated that the expression of ClLOX genes may be influenced by stress and plant hormones. Bioinformatic and expression analyses revealed that the expression of ClLOX genes is tissue-specific and hormone-responsive. The detected LOX genes exhibited distinctive expression patterns in various tissues. Different ClLOX genes showed different responses to methyl jasmonate (MeJA), salicylic acid (SA), and ethylene (ET) treatments, particularly ClLOX7, which exhibited the most active response to the above treatments. This study provides valuable information for a better understanding of the functions of LOX genes and further exploration of the LOX gene family in watermelon.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comprehensive Genomic Characterization and Expression Analysis of the Lipoxygenase Gene Family in Watermelon under Hormonal Treatments

Liu

Zhou

et al. 2020

Agriculture

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“…Plant oxylipins, including GLV and hormone JA, play a key role in signaling molecules to induce defense responses against pathogens [27]. To better elucidate the role of oxylipin-mediated crosstalk in the plant-fungal interaction of maize germinating kernels and F. verticillioides, a functional analysis was performed with maize mutants carrying mutator insertions in the ZmLOX4 gene in comparison with the wild-type inbred line W22 and the resistant genotype Tzi18.…”

Section: Discussionmentioning

confidence: 99%

“…When maize is attacked by F. verticillioides, an intracellular signaling cascade is initiated, including the activation of hormones that in turn target transcription factor families, promoting the downstream switching on of defense responsive genes for pathogenesisrelated proteins phenylpropanoid metabolism and lipoxygenases (LOX) [21][22][23][24][25][26]. Plant LOX enzymes synthesize several signaling molecules, named oxylipins, and depending on their regiospecificity, are classified in two major subfamilies, 9-LOX and 13-LOX [27]. In the 9-LOX pathway, polyunsaturated fatty acids (PUFA) are converted to hydroperoxyoctadecadienoic acid (HPOD), hydroperoxy-octadecatrienoic acid (HPOT) and 9S-keto octadecatrienoic acid (9-KOT), most of them involved in defense against pathogens.…”

Section: Introductionmentioning

confidence: 99%

Loss of ZmLIPOXYGENASE4 Decreases Fusarium verticillioides Resistance in Maize Seedlings

Lanubile

Borrelli

Soccio

et al. 2021

Genes

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Fusarium verticillioides is one of the most relevant fungal species in maize responsible for ear, stalk and seedling rot, as well as the fumonisin contamination of kernels. Plant lipoxygenases (LOX) synthesize oxylipins that play a crucial role in the regulation of defense mechanisms against pathogens and influence the outcome of pathogenesis. To better uncover the role of these signaling molecules in maize resistance against F. verticillioides, the functional characterization of the 9-LOX gene, ZmLOX4, was carried out in this study by employing mutants carrying Mu insertions in this gene (named as UFMulox4). In this regard, the genotyping of five UFMulox4 identified the mutant UFMu10924 as the only one having an insertion in the coding region of the gene. The impact of ZmLOX4 mutagenesis on kernel defense against F. verticillioides and fumonisin accumulation were investigated, resulting in an increased fungal susceptibility compared to the inbred lines W22 and Tzi18. Moreover, the expression of most of the genes involved in the LOX, jasmonic acid (JA) and green leaf volatiles (GLV) pathways, as well as LOX enzymatic activity, decreased or were unaffected by fungal inoculation in the mutant UFMu10924. These results confirm the strategic role of ZmLOX4 in controlling defense against F. verticillioides and its influence on the expression of several LOX, JA and GLV genes.

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Post‐harvest ultraviolet irradiation induces changes in physical–chemical properties and levels of polycyclic aromatic hydrocarbons and gene expression in mulberry fruit

Ghafari,

Hassanpour,

Motafakkerazad

2023

J Sci Food Agric

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BackgroundEarlier studies have reported that post‐harvest UV irradiation can increase health‐promoting compound contents, but the effects of UV irradiation on the possibility of reducing polycyclic aromatic hydrocarbons (PAHs) contents in mulberries are still less known. Fruits of black mulberry were exposed to two UV illumination dosages (3.5 and 7 kJ/m2) and stored for 4, 8, and 12 days.ResultsMulberries treated showed higher antioxidant enzymes activity and phenolic compounds content compared to control. The transcription factors (TFs) MdoMYB121, MdoMYB155, MdbZIP2, and MdbZIP48 were highly expressed in two UV illumination dosages (about 45–95% higher than control). The fluorine (Flu) naphthalene (Nap) content in treated fruits decreased by 21–85% compared to control.ConclusionThe findings of this study indicate that UV irradiation can be considered a promising technique to some PAHs removal and increase the health‐promoting potential and indirectly to ameliorate the aesthetic value due to desirable colour parameters.This article is protected by copyright. All rights reserved.

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Plant Lipoxygenases and Their Role in Plant Physiology

Cited by 133 publications

References 105 publications

Comprehensive Genomic Characterization and Expression Analysis of the Lipoxygenase Gene Family in Watermelon under Hormonal Treatments

Comprehensive Genomic Characterization and Expression Analysis of the Lipoxygenase Gene Family in Watermelon under Hormonal Treatments

Loss of ZmLIPOXYGENASE4 Decreases Fusarium verticillioides Resistance in Maize Seedlings

Post‐harvest ultraviolet irradiation induces changes in physical–chemical properties and levels of polycyclic aromatic hydrocarbons and gene expression in mulberry fruit

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