Plant lectins as defense proteins against phytophagous insects

Vandenborre, Gianni; Smagghe, Guy; Damme, Els J.M. Van

doi:10.1016/j.phytochem.2011.02.024

Cited by 303 publications

(245 citation statements)

References 112 publications

Supporting

Mentioning

226

Contrasting

Unclassified

Order By: Relevance

“…This type of proteins is characterized by the unique property of initiating reversible and specific binding with carbohydrate structures. By the overall domain architectures, plant lectins are distinguished into merolectins, hololectins, superlectins and chimerolectins, among which the first three types, respectively, possess only one, two or more identical/homologous and two or more different carbohydrate-binding domains while chimerolectins, the last type, have at least one carbohydrate-binding domains that are fused to a domain with different biological functions 1 . Plant lectins/hemagglutinins could defend against plant attackers through their interactions with the glycoconjugates present in organisms 1 .…”

Section: Discussionmentioning

confidence: 99%

“…Mammals like human beings are protected by a strong immune system from the frequent attacks by ubiquitous pathogens. Though the defending mechanism of plants is not as distinct as that in mammals, they definitely have a defense system against pathogens and plants under threat can react with alterations in morphology, structural features and production of chemicals to augment resistance against the threat 1 . The expression of defense proteins, like lectins/hemagglutinins 2,3 , anti-fungal proteins 4 , ribosome inactivating proteins 5 , protease inhibitors 6 , etc., is one of the common strategies adopted by plants.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Two legume defense proteins suppress the mobility of nasopharyngeal carcinoma cells

Dan

2016

Journal of Enzyme Inhibition and Medicinal Chemistry

View full text Add to dashboard Cite

A 16-kDa trypsin inhibitor was isolated from an edible legume using various chromatographic procedures. The protein was unadsorbed on Affi-gel blue gel but adsorbed on DEAE-Sepharose and Mono Q following which media the protein was subsequently subjected to gel filtration on Superdex 75 and a final 21-fold purification was achieved. This trypsin inhibitor showed remarkable pH and thermal stability. Its inhibitory activity was impaired in the presence of 1 mM dithiothreitol. The anti-proliferative and anti-mobility activities of this trypsin inhibitor and a hemagglutinin isolated from the same legume were tested on nasopharyngeal carcinoma cells. These two defense proteins demonstrated discrepant anti-proliferative efficacies that the hemagglutinin could greatly suppress the proliferation of nasopharyngeal carcinoma cells, while the trypsin inhibitor revealed a minor effect. However, these two proteins could both attenuate the mobility of nasopharyngeal carcinoma cells. The present study revealed the potential of applying plant defense proteins in cancer treatment.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Two legume defense proteins suppress the mobility of nasopharyngeal carcinoma cells

Dan

2016

Journal of Enzyme Inhibition and Medicinal Chemistry

View full text Add to dashboard Cite

show abstract

“…Outras enzimas antioxidantes importantes incluem lipoxigenases, fenilalanina amônia liase, superóxido dismutase, entre outras. Indução de enzimas antioxidantes em plantas devido à herbivoria tem recebido atenção considerável nos últimos anos (HENG-MOSS et al, 2004;CHEN et al, 2009;RANI & JYOTHSNA, 2010;VANDENBORRE et al, 2011;WAR et al, 2011a;WAR et al, 2011b).…”

Section: Enzimas Antioxidantesunclassified

O Papel Das Enzimas Antioxidantes Na Defesa Das Plantas Contra Insetos Herbívoros E Fitopatógenos

Nascimento¹,

Barrigossi²

2014

Agrarian Academy

View full text Add to dashboard Cite

RESUMOMuitas plantas passam por mudanças bioquímicas significativas em resposta ao ataque de estressores bióticos, como patógenos ou insetos herbívoros. Ambos estressores bióticos quando atacam as plantas fazem com que estas respondam de forma rápida com uma "explosão oxidativa", que constituem na produção de espécies reativas de oxigênio (ERO), principalmente ânion superóxido (O 2•-) e peróxido de hidrogênio (H 2 O 2 ). O acúmulo destas substâncias nas células vegetais pode ser tóxico para ambos. Estas EROs acionam moléculas para a indução de genes de defesa e a polimerização de proteínas que compõe a parede celular, além de estimular a produção de enzimas antioxidativas ou de limpeza. Existe uma variedade destas enzimas que irão decompor as espécies reativas de oxigênio. Como por exemplo, temos superóxido dismutase (SOD), peroxidase (POX), catalase (CAT), polifenol oxidase (PPO), entre outras. Nesta revisão será abordado como a herbivoria e a patogenicidade desencadeiam uma explosão oxidativa ativando o sistema de defesa da planta com a ação das enzimas antioxidativas. PALAVRAS-CHAVE: catalase, peroxidase, estresse biótico, inseto, fungo. THE ROLE OF ANTIOXIDANT ENZYMES IN PLANT DEFENSE AGAINST HERBIVOROUS INSECTS AND PHYTOPATHOGENSABSTRACT Many plants suffer significant biochemical changes in response the attack of biotic stressors, such as phytopathogens or herbivorous insects. When any of these biotic stressors attack plants, this causes quick responses via an "oxidative burst", which constitutes the production of reactive oxygen species (ROS), particularly superoxide anion (O 2•-) and hydrogen peroxide (H 2 O 2 ). The accumulation of these substances in vegetal cells can be toxic to both the biotic stressors and plants. These ROS will trigger molecules for the induction of defense genes and polymerization of proteins that makes up the cell walls, as well as stimulate the production of antioxidant enzymes or scavenging. There are a variety of these enzymes that will decompose the reactive oxygen species; the superoxide dismutase (SOD), peroxidase (POX), catalase (CAT) and polyphenol oxidase (PPO) are some examples. This review will describe how herbivory and pathogenic triggers cause an oxidative burst stimulating the defense system of the plants with the actions of antioxidant enzymes.

show abstract

“…Among plant defense proteins are protease inhibitors (PIs) and lectins, which are found in vegetal tissues, mainly in reserve organs, and usually act against insects, bacteria and fungi (Peumans and Van Damme, 1995;Dunaevsky et al, 2005). Lectins and PIs affect the digestive process of insects by reducing the breakdown of the ingested proteins into amino acids (Murdock and Shade, 2002;Macedo et al, 2004;Vandenborre et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Plant antiherbivore defenses in Fabaceae species of the Chaco

2016

View full text Add to dashboard Cite

The establishment and maintenance of plant species in the Chaco, one of the widest continuous areas of forests in the South American with sharp climatic variations, are possibly related to biological features favoring plants with particular defenses. This study assesses the physical and chemical defenses mechanisms against herbivores of vegetative and reproductive organs. Its analyses of 12 species of Fabaceae (Leguminosae) collected in remnants of Brazilian Chaco shows that 75% present structural defense characters and 50% have chemical defense -defense proteins in their seeds, like protease inhibitors and lectins. Physical defenses occur mainly on branches (78% of the species), leaves (67%), and reproductive organs (56%). The most common physical characters are trichomes and thorns, whose color represents a cryptic character since it does not contrast with the other plant structures. Defense proteins occur in different concentrations and molecular weight classes in the seeds of most species. Protease inhibitors are reported for the first time in seeds of: Albizia niopoides, Anadenanthera colubrina, Mimosa glutinosa, Prosopis rubriflora, and Poincianella pluviosa. The occurrence of physical and chemical defenses in members of Fabaceae indicate no associations between defense characters in these plant species of the Chaco.Keywords: structural characters, protease inhibitors, lectins, defense mechanisms. Defesas das plantas anti-herbivoria em espécies de Fabaceae do Chaco ResumoO estabelecimento e a manutenção de espécies no Chaco, uma planície semi-árida da América do Sul com variações climáticas importantes, possivelmente estão relacionados a características biológicas que favorecem as plantas detentoras de defesas particulares. Este estudo teve como objetivos avaliar os mecanismos de defesa física e química anti-herbivoria em órgãos vegetativos e reprodutivos. Analisamos 12 espécies da família Fabaceae (Leguminosae) obtidas em remanescentes de Chaco brasileiro. Observamos que 75% das espécies estudadas apresentam atributo de defesa física e 50% possuem defesa química -proteínas de defesa nas sementes, como inibidores de protease e lectinas. As defesas físicas ocorrem principalmente nos ramos (78% das espécies), nos órgãos reprodutivos (56% das espécies) e nas folhas (67%). Os atributos físicos mais frequentes são tricomas e espinhos, cuja coloração não contrastante com as demais estruturas das plantas representa um caráter críptico. Proteínas de defesa ocorrem nas sementes da maioria das espécies, com diferentes concentrações e classes de pesos moleculares. Inibidores de protease nas sementes estão sendo relatados pela primeira vez em: Albizia niopoides, Anadenanthera colubrina, Mimosa glutinosa, Prosopis rubriflora e Poincianella pluviosa. A ocorrência de defesas física e química entre os membros de Fabaceae indica que não há associações entre as características de defesa das espécies de plantas avaliadas no Chaco.Palavras-chave: características estruturais, inibidores de protease, lectinas, mecanismo...

show abstract

Plant lectins as defense proteins against phytophagous insects

Cited by 303 publications

References 112 publications

Two legume defense proteins suppress the mobility of nasopharyngeal carcinoma cells

Two legume defense proteins suppress the mobility of nasopharyngeal carcinoma cells

O Papel Das Enzimas Antioxidantes Na Defesa Das Plantas Contra Insetos Herbívoros E Fitopatógenos

Plant antiherbivore defenses in Fabaceae species of the Chaco

Contact Info

Product

Resources

About