Plant isoflavones can prevent adverse effects of benzene on porcine ovarian activity: an in vitro study

Sirotkin, Alexander V.; Záhoranska, Zuzana; Tarko, Adam; Popovska-Percinic, Florina; Alwasel, Saleh; Harrath, Abdel Halim

doi:10.1007/s11356-020-09260-8

Cited by 14 publications

(11 citation statements)

References 29 publications

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“…In addition, the antioxidant and phytoestrogenic properties of TT saponins, including diosgenin and flavonoids, including rutin (Chhatre et al, 2014) could, in turn, promote cell proliferation and prevent DNA fragmentation, resulting in apoptosis. Such an activity has been observed in previous experiments: diosgenin and rutin were able to increase proliferation of cultured porcine ovarian granulosa cells (Sirotkin, Alexa, et al, 2019; Sirotkin, Záhoranska, et al, 2020). Furthermore, in our study, TT promoted ovarian cell proliferation and did not affect apoptosis, which, in turn, can result in the promotion of ovarian follicular growth and development (Sirotkin, 2014).…”

Section: Discussionsupporting

confidence: 77%

Section: The Effect Of Tribulus Terrestris On Ovarian Cell Functionssupporting

confidence: 82%

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Tribulus terrestris can suppress the adverse effect of toluene on bovine and equine ovarian granulosa cells

Fabová

Tarko

Harrath

et al. 2022

Reprod Domestic Animals

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Influence of oil-related product toluene and herbal remedy puncturevine Tribulus terrestris L. (TT) on female reproduction is known. Yet, mechanisms of their action on ovaries in different species and potential protective effect of TT against adverse toluene action remain to be established. We studied the effect of toluene, TT, and their combination on ovarian granulosa cells from two mammalian species (cows and horses). Viability, markers of proliferation (PCNA) and apoptosis (bax), steroid hormones, IGF-I, oxytocin, and prostaglandin F (PGF) release were analyzed by trypan blue exclusion test, quantitative immunocytochemistry, and EIA/ELISA. Toluene suppressed all analyzed parameters. In both species, TT stimulated proliferation and reduced progesterone, oxytocin, and PGF. In horses, TT inhibited testosterone and IGF-I. In both species, TT supported toluene effect on viability, steroids, IGF-I, and PGF, and inverted its action on apoptosis. In cows, TT promoted toluene effect on proliferation. In horses, TT supported toluene effect on oxytocin but suppressed its influence on proliferation. In both species, toluene induced inhibitory action of TT on viability, steroids, IGF-I, and PGF, and prevented its stimulatory action on proliferation. In cows, toluene supported inhibitory action of TT on oxytocin and prevented its stimulatory action on apoptosis. In horses, toluene induced stimulatory effect of TT on apoptosis. Our results indicate potential toxic toluene effect on farm animal ovaries, applicability of TT as a biostimulator of farm animal reproduction and as a protector against the adverse influence of toluene on female reproduction.

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Section: Discussionsupporting

confidence: 77%

Section: The Effect Of Tribulus Terrestris On Ovarian Cell Functionssupporting

confidence: 82%

Tribulus terrestris can suppress the adverse effect of toluene on bovine and equine ovarian granulosa cells

Fabová

Tarko

Harrath

et al. 2022

Reprod Domestic Animals

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confidence: 66%

“…Ovarian function mainly manifests as endocrine function and follicular atresia [26]. E 2 and P 4 levels and granulosa cell senescence are classical criteria for ovarian senescence [27]. This study demonstrated that in vitro culturing of intact antral follicles within 36 h caused atresia, although morphology remained intact; Assessment of cell status revealed that cell viability was higher within 24 h and E 2 and P 4 hormone levels were also at normal levels, thus establishing that oxidative stress stimulation of follicles within 24 h of in vitro culture induces senescence.…”

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confidence: 99%

Establishment of porcine ovarian follicles treated with t-BHP for an in vitro aging model

Shi

Gao

Zhao

et al. 2022

Preprint

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Ovarian aging is closely associated with low female fertility. Excessive oxidative stress can induce ovarian senescence and follicular atresia, thereby reducing reproductive performance. In this study, intact antral follicles of pigs were treated with tert-butyl hydroperoxide (t-BHP) to simulate aging stimulation conditions. The results showed that 200 μM t-BHP induced the senescence-related phenotype of antral follicles, which was time-dependent and changed significantly after 6 h of treatment. t-BHP can induce an increase in reactive oxygen species (ROS) and senescence mediated by Caspase-3, P53, Foxo1, and SOD. Senescence-associated β-galactosidase (SA-β-Gal) staining revealed an increase in the number of positive cells. Enzyme-linked immunosorbent assays (ELISA) showed that the level of reactive oxygen species increased, and that the ratio of progesterone (P4) to estradiol (E2) increased. Finally, the findings of this study showed that the relative expression levels of Caspase-3, P53, Foxo1 mRNA, and protein increased, while the relative expression levels of SOD decreased. In conclusion, treatment with 200 μM of t-BHP effectively induced follicular senescence at 6 h. Therefore, the in vitro treatment of follicles with 200 μM t-BHP for 6 h may be a feasible in vitro culture model to simulate ovarian senescence in gilts with high parity.

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“…In vivo and in vitro studies on rats demonstrated the ability of rutin to promote the plasma luteinizing hormone (LH) level, expression of ovarian follicle-stimulating hormone (FSH) receptors, steroidogenic enzymes, ovarian folliculogenesis and fecundity [90,91]. In in vitro experiments with cultured porcine granulosa cells, the addition of rutin was able to promote 17β-estradiol and testosterone, but not progesterone release [8,89,97]. It is not to be excluded that the increase in granulosa cell viability under the influence of elderberry extract could be due to the upregulation of estradiol, a known promoter of ovarian cell viability and ovarian folliculogenesis [98].…”

Section: Effect Of Elderberry and Its Constituents On Ovarian Cell St...mentioning

confidence: 99%

Black Elder and Its Constituents: Molecular Mechanisms of Action Associated with Female Reproduction

et al. 2022

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The present review summarizes the current knowledge concerning provenance, properties, physiological and therapeutic actions of elderberry and the bioactive molecules present in the plant, with emphasis on their action on female reproduction. Elderberry or black elder (Sambucus nigra L.) attracts attention due to its easy cultivation and high availability of bioactive compounds. Most of the available data concerning black elder’s therapeutic action are focused on its effects such as activation of immune processes and anti-inflammatory processes (cytokine production, etc.) and regulation of hormones and their receptors in cancer cells. The effects of elderberry on reproduction have been poorly investigated so far. Nevertheless, conducted studies so far demonstrate the stimulatory influence of black elder extract and its constituents, such as rutin, anthocyanins and agglutinins, on the viability and steroidogenesis of healthy ovarian cells as well as their ability to promote apoptosis and reduce the viability and proliferation of ovarian cancer cells. Furthermore, the action of black elder extract and its constituent biomolecules, such as anthocyanins and lectins, on embryogenesis and the embryonal estradiol-estradiol receptor system have also been reported. The available information, despite limitations, suggest the applicability of black elder constituents for improvement of reproductive processes in animal biotechnology, animal production and assisted reproduction, as well as for prevention and treatment of reproductive disorders (including cancer) in veterinary and human medicine.

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Plant isoflavones can prevent adverse effects of benzene on porcine ovarian activity: an in vitro study

Cited by 14 publications

References 29 publications

Tribulus terrestris can suppress the adverse effect of toluene on bovine and equine ovarian granulosa cells

Tribulus terrestris can suppress the adverse effect of toluene on bovine and equine ovarian granulosa cells

Establishment of porcine ovarian follicles treated with t-BHP for an in vitro aging model

Black Elder and Its Constituents: Molecular Mechanisms of Action Associated with Female Reproduction

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