Plant growth regulator interactions in physiological processes for controlling plant regeneration and in vitro development of Tulbaghia simmleri

Kumari, Aloka; Baskaran, P.; Plačková, Lenka; Omámiková, Hana; Nisler, Jaroslav; Doležal, Karel; Staden, J. Van

doi:10.1016/j.jplph.2018.01.005

Cited by 29 publications

(27 citation statements)

References 35 publications

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“…Genotypes differ in their levels of endogenous phytohormones. In vitro shoot regeneration is controlled by the exogenous cytokinin and auxin balances, therefore the different levels of endogenous phytohormones is assumed to be the main cause of variation between genotypes with different degrees of competence (Kumari et al 2018). The high regeneration rate as well as high survival rate of acclimatized plants (95.5%) confirmed the appropriate selection of the PGRs as well as cultivation and micropropagation technique.…”

Section: Discussionmentioning

confidence: 98%

Phytochemical variability during vegetation of Chamerion angustifolium (L.) Holub genotypes derived from in vitro cultures

Dreger

Seidler-Łożykowska

Szalata

et al. 2021

Plant Cell Tiss Organ Cult

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The purpose of the study was to evaluate Chamerion angustifolium (L.) Holub genotypes for preliminary selection and further breeding programs aimed at obtaining a suitable industrial form for the pharmaceutical applications. Clonally propagated plants representing 10 genotypes of Ch. angustifolium were regenerated under in vitro conditions, hardened and planted in the field. Studies included an evaluation of shoot proliferation, phytochemical assessment of in vitro and ex vitro plants as well as investigations of intraspecies variability regarding four phenological stages: vegetative, beginning of blooming, full blooming, and green fruit phases. Quantitative and qualitative analyses of bioactive compounds were performed using high-performance liquid chromatography coupled with diode array detector and tandem mass spectrometer (HPLC–DAD–MS/MS) and high-performance liquid chromatography (HPLC) methods. The efficiency of shoot multiplication varied between genotypes from 8.12 to 21.48 shoots per explant. A high reproduction rate (> 20 shoots per explant) was recorded for four lines (PL_45, PL_44, PL_58, DE_2). Plants grown in vitro synthesized oenothein B (11.2–22.3 mg g−1 DW) and caffeic acid derivatives. Plants harvested from field contained the full spectrum of polyphenols characteristic for this species, and oenothein B and quercetin 3-O-glucuronide were the most abundant. The maximal content of oenothein B was determined in the vegetative phase of fireweed, while some flavonoids were found in the highest amount in full blooming phase. The results of analysis of variance indicated significant differences among genotypes in oenothein B, 3-O-caffeoylquinic acid and flavonoids accumulation in four phenological phases. PL_44 plants were characterized by high content of oenothein B and quercetin 3-O-glucuronide as well as a relatively high level of other flavonoids. Based on our phytochemical and micropropagation studies, PL_44 genotype was the best candidate for early selection and further breeding programs.

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Section: Discussionmentioning

confidence: 98%

Phytochemical variability during vegetation of Chamerion angustifolium (L.) Holub genotypes derived from in vitro cultures

Dreger

Seidler-Łożykowska

Szalata

et al. 2021

Plant Cell Tiss Organ Cult

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show abstract

“…Unfortunately, understanding of the biochemistry and metabolism of Cannabis beyond the cannabinoid pathways is limited and represents a necessary area of future study. To date, several studies [120][121][122][123][124] showed that the gene expression pattern of endogenous PGRs and the balance between endogenous and exogenous PGRs play an important role in regeneration efficiency, especially in recalcitrant plants. For instance, Kumari et al [120] studied the endogenous level of PGRs via UHPLC-MS analysis and its effect on shoot regeneration and somatic embryogenesis of Tulbaghia simmleri.…”

Section: Future Directionsmentioning

confidence: 99%

“…To date, several studies [120][121][122][123][124] showed that the gene expression pattern of endogenous PGRs and the balance between endogenous and exogenous PGRs play an important role in regeneration efficiency, especially in recalcitrant plants. For instance, Kumari et al [120] studied the endogenous level of PGRs via UHPLC-MS analysis and its effect on shoot regeneration and somatic embryogenesis of Tulbaghia simmleri. They reported that endogenous phytohormone signaling and transport affects In Vitro biological processes related to auxin and cytokinin, and generally, high-frequency regeneration protocols can be achieved when there is a balance between endogenous and exogenous PGRs.…”

Section: Future Directionsmentioning

confidence: 99%

The Past, Present and Future of Cannabis sativa Tissue Culture

Monthony

Page

Hesami

et al. 2021

Plants

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The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies for the species. Micropropagation affords researchers and producers methods to rapidly propagate insect-/disease-/virus-free clonal plants and store germplasm and forms the basis for other biotechnologies. Despite this need, research in the area is limited due to the long history of prohibitions and restrictions. Existing literature has multiple limitations: many publications use hemp as a proxy for drug-type Cannabis when it is well established that there is significant genotype specificity; studies using drug-type cultivars are predominantly optimized using a single cultivar; most protocols have not been replicated by independent groups, and some attempts demonstrate a lack of reproducibility across genotypes. Due to culture decline and other problems, the multiplication phase of micropropagation (Stage 2) has not been fully developed in many reports. This review will provide a brief background on the history and botany of Cannabis as well as a comprehensive and critical summary of Cannabis tissue culture. Special attention will be paid to current challenges faced by researchers, the limitations of existing Cannabis micropropagation studies, and recent developments and future directions of Cannabis tissue culture technologies.

show abstract

“…To date, several studies [120][121][122][123][124] showed that the gene expression pattern of endogenous PGRs and the balance between endogenous and exogenous PGRs play an important role in regeneration efficiency, especially in recalcitrant plants. For instance, Kumari et al [120] studied the endogenous level of PGRs via UHPLC-MS analysis and its effect on shoot regeneration and somatic embryogenesis of Tulbaghia simmleri. They reported that endogenous phytohormone signaling and transport effects in vitro biological processes related to auxin and cytokinin, and, generally, high-frequency regeneration protocols can be achieved when there is a balance between endogenous and exogenous PGRs.…”

Section: Future Directionsmentioning

confidence: 99%

The Past, Present and Future of Cannabis sativa Tissue Culture

Monthony¹,

Page²,

Hesami³

et al. 2020

Preprint

View full text Add to dashboard Cite

The recent legalization of Cannabis sativa L. in many regions has revealed a need for effective propagation and biotechnologies for the species. Micropropagation affords researchers and producers methods to rapidly propagate insect/disease/virus free clonal plants, store germplasm, and forms the basis for other biotechnologies. Despite this need, research in the area is limited due to the long history or prohibitions and restrictions. Existing literature has multiple limitation: many publications use hemp as a proxy for drug-type Cannabis when it is well established that there is significant genotype specificity, studies using drug-type cultivars are predominantly op-timized using a single cultivar, most protocols have not been replicated by independent groups, and some attempts demonstrate a lack of reproducibility across genotypes. Due to culture decline and other problems the multiplication phase of micropropagation (stage 2) has not been fully developed in many reports. This review will provide a brief background on the history and botany of Cannabis as well as a comprehensive and critical summary of Cannabis tissue culture. Special attention will be paid to current challenges faced by researchers, the limitations of existing Cannabis micropropagation studies, and recent developments and future directions of Cannabis tissue culture technologies.

show abstract

Plant growth regulator interactions in physiological processes for controlling plant regeneration and in vitro development of Tulbaghia simmleri

Cited by 29 publications

References 35 publications

Phytochemical variability during vegetation of Chamerion angustifolium (L.) Holub genotypes derived from in vitro cultures

Phytochemical variability during vegetation of Chamerion angustifolium (L.) Holub genotypes derived from in vitro cultures

The Past, Present and Future of Cannabis sativa Tissue Culture

The Past, Present and Future of Cannabis sativa Tissue Culture

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