Plant Growth-Promoting Rhizobacteria for Cannabis Production: Yield, Cannabinoid Profile and Disease Resistance

Lyu, Dongmei; Backer, Rachel; Robinson, Wilfrid; Smith, Donald L.

doi:10.3389/fmicb.2019.01761

Cited by 53 publications

(34 citation statements)

References 89 publications

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“…Novel strategies to boost plant growth and combat plant diseases in agriculture are currently being investigated with the aim of replacing current procedures with more sustainable approaches 6 . Many PGPB, such as Pseudomonas spp., act as biological control agents against plant diseases 40 , produce compounds including hormones, antibiotics, polysaccharides and siderophores, induce ISR in plants and increase their abilitiy to defend against pathogens [41][42][43] .…”

Section: Discussionmentioning

confidence: 99%

Plant growth-promoting activity and quorum quenching-mediated biocontrol of bacterial phytopathogens by Pseudomonas segetis strain P6

Rodríguez

Torres

Blanco

et al. 2020

Sci Rep

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Given the major threat of phytopathogenic bacteria to food production and ecosystem stability worldwide, novel alternatives to conventional chemicals-based agricultural practices are needed to combat these bacteria. the objective of this study is to evaluate the ability of Pseudomonas segetis strain P6, which was isolated from the Salicornia europaea rhizosphere, to act as a potential biocontrol agent given its plant growth-promoting (pGp) and quorum quenching (QQ) activities. Seed biopriming and in vivo assays of tomato plants inoculated with strain P6 resulted in an increase in seedling height and weight. We detected QQ activity, involving enzymatic degradation of signal molecules in quorum sensing communication systems, against a broad range of N-acylhomoserine lactones (AHLs). HpLc-MRM data and phylogenetic analysis indicated that the QQ enzyme was an acylase. the QQ activity of strain P6 reduced soft rot symptoms caused by Dickeya solani, Pectobacterium atrosepticum and P. carotovorum on potato and carrot. In vivo assays showed that the PGP and QQ activities of strain P6 protect tomato plants against Pseudomonas syringae pv. tomato, indicating that strain P6 could have biotechnological applications. To our knowledge, this is the first report to show PGP and QQ activities in an indigenous Pseudomonas strain from Salicornia plants. Plant bacterial pathogens cause diseases in a wide range of crops worldwide and considerable economic losses in agriculture 1,2. Antibiotics and chemical pesticides have been used for many decades to combat plant bacterial infections 3,4. However, stricter legislation has been introduced in recent years regarding the use of chemical-based treatments which have caused serious problems such as reduced productivity due to resistance to treatment, soil salinization and environmental pollution 5. As a consequence, alternative strategies to combat plant diseases and to promote plant growth are required in order to replace current procedures with more sustainable eco-friendly approaches 6. Currently, one of the most promising tools used in the agricultural industry is the use of formulations containing plant growth-promoting bacteria (PGPB), also known as plant growth-promoting rhizobacteria (PGPR). These are beneficial microorganisms that act as biofertilizers and can fight plant pathogens 7,8. They counteract pathogens through physical displacement, siderophore production, as well as the synthesis of antibiotics, bacteriocins and hydrolytic enzymes which inhibit pathogen growth 8,9. They also boost plant resistance to infections, which is also called induced systemic resistance (ISR) 10 , through mechanisms such as callose deposition 11. PGPB, which promote processes such as plant growth and stress tolerance, and can fight phytopathogens, are considered to be an effective, sustainable and environmentally-friendly alternative to be used in agriculture 8,12. Although several members of the genus Pseudomonas have been identified as PGPB, only a few have been isolated from

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Section: Discussionmentioning

confidence: 99%

Plant growth-promoting activity and quorum quenching-mediated biocontrol of bacterial phytopathogens by Pseudomonas segetis strain P6

Rodríguez

Torres

Blanco

et al. 2020

Sci Rep

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“…Cannabinoid and terpene production by plants is linked to both attraction of pollinators and responses to plant pathogens (Penuelas et al 2014; Andre et al 2016; Allen et al 2019; Lyu et al 2019). Cannabis plants are classified into 5 categories reflecting their THCA, CBDA, and CBGA expression.…”

Section: Introductionmentioning

confidence: 99%

Sequence and annotation of 42 cannabis genomes reveals extensive copy number variation in cannabinoid synthesis and pathogen resistance genes

McKernan

Helbert

Kane

et al. 2020

Preprint

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Cannabis is a diverse and polymorphic species. To better understand cannabinoid synthesis inheritance and its impact on pathogen resistance, we shotgun sequenced and assembled a Cannabis trio (sibling pair and their offspring) utilizing long read single molecule sequencing. This resulted in the most contiguous Cannabis sativa assemblies to date. These reference assemblies were further annotated with full-length male and female mRNA sequencing (Iso-Seq) to help inform isoform complexity, gene model predictions and identification of the Y chromosome. To further annotate the genetic diversity in the species, 40 male, female, and monoecious cannabis and hemp varietals were evaluated for copy number variation (CNV) and RNA expression. This identified multiple CNVs governing cannabinoid expression and 82 genes associated with resistance to Golovinomyces chicoracearum, the causal agent of powdery mildew in cannabis. Results indicated that breeding for plants with low tetrahydrocannabinolic acid (THCA) concentrations may result in deletion of pathogen resistance genes. Low THCA cultivars also have a polymorphism every 51 bases while dispensary grade high THCA cannabis exhibited a variant every 73 bases. A refined genetic map of the variation in cannabis can guide more stable and directed breeding efforts for desired chemotypes and pathogen-resistant cultivars. Sequence and annotation of 42 cannabis genomes reveals extensive copy number variation in cannabinoid synthesis and pathogen resistance genes

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“…Additional studies about the biological characteristics of strain ZSH-1, such as colonization, survival ability, and product formulation, are needed to confirm the biocontrol effect of strain ZSH-1 in the field (Liu et al 2019). Meanwhile, the abilities of strain ZSH-1 to promote plant growth and activate induced systemic resistance (ISR) of plant in the field should also be studied in the future (Lyu et al 2019;Tiwari et al 2019, andJinal andAmaresan 2020).…”

Section: Cell Wall-degrading Traitsmentioning

confidence: 99%

Biological control of poplar anthracnose caused by Colletotrichum gloeosporioides (Penz.) Penz. & Sacc.

Huang

Tian

Huang

et al. 2020

Egypt J Biol Pest Control

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Poplar anthracnose is one of the most serious diseases caused by the fungal pathogen Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. Biocontrol is an efficient green way for the disease control, and numerous researches have focused on exploring the potential biocontrol bacteria strains against C. gloeosporioides. In this study, antifungal activities against C. gloeosporioides of 108 rhizosphere soil isolates from healthy polar plants were investigated in vitro by the dual culture assay. The results suggested that strain ZSH-1 showed the highest level of antifungal activity, as it inhibited C. gloeosporioides at a distance of 10.00 mm. Based on the morphological, physiological-biochemical characteristics, and phylogeny analysis, strain ZSH-1 was identified as Bacillus subtilis. The sterile culture filtrate, crude protein, and crude lipopeptide extracts from the culture filtrate, and volatile compound(s) of ZSH-1 displayed a strong antagonism towards 7 fungal phytopathogens (C. gloeosporioides, Fusarium oxysporum, Alternaria tenuissima, Cytospora chrysosperma, Botryosphaeria dothidea, Mucor sp., and Absidia sp.), with inhibition rates ranging from 44.0 to 89.1%, 26.7 to 85.4%, 11.6 to 89.7%, and 7.8 to 63.2%, respectively. Moreover, ZSH-1 exhibited cell wall-degrading traits by producing 3 lytic enzymes (cellulose, β-1,3-glucanase, and protease). Finally, the greenhouse studies also revealed that strain ZSH-1 had a 47.6% (12 days) efficacy in controlling poplar anthracnose when compared with the control. In concluding, obtained results demonstrate the potential biocontrol effect of B. subtilis ZSH-1, and it can be used as a promising biocontrol agent against poplar anthracnose and other fungal phytopathogens.

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Plant Growth-Promoting Rhizobacteria for Cannabis Production: Yield, Cannabinoid Profile and Disease Resistance

Cited by 53 publications

References 89 publications

Plant growth-promoting activity and quorum quenching-mediated biocontrol of bacterial phytopathogens by Pseudomonas segetis strain P6

Plant growth-promoting activity and quorum quenching-mediated biocontrol of bacterial phytopathogens by Pseudomonas segetis strain P6

Sequence and annotation of 42 cannabis genomes reveals extensive copy number variation in cannabinoid synthesis and pathogen resistance genes

Biological control of poplar anthracnose caused by Colletotrichum gloeosporioides (Penz.) Penz. & Sacc.

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