Plant Growth-Promoting Pseudomonas putida WCS358 Produces and Secretes Four Cyclic Dipeptides: Cross-Talk with Quorum Sensing Bacterial Sensors

Degrassi, Giuliano; Aguilar, Claudio; Bosco, Marcella; Zahariev, Sotir; Pongor, Sándor; Venturi, Vittorio

doi:10.1007/s00284-002-3704-y

Cited by 173 publications

(147 citation statements)

References 13 publications

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“…This regulatory mechanism is known as quorum sensing, and it can be mediated by different signal molecules. In most gram-negative bacteria where it has been described, it is mediated by AHLs, although other molecules such as quinolones or cyclic dipeptides also act as quorum-sensing signals (11,29). AHLs are found in different bacteria and regulate processes such as bio- (33).…”

Section: Discussionmentioning

confidence: 99%

Cell Density-Dependent Gene Contributes to Efficient Seed Colonization by Pseudomonas putida KT2440

Espinosa‐Urgel

Ramos

2004

Appl Environ Microbiol

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We have characterized the expression pattern of a gene, ddcA, involved in initial colonization of corn seeds by Pseudomonas putida KT2440. The ddcA gene codes for a putative membrane polypeptide belonging to a family of conserved proteins of unknown function. Members of this family are widespread among prokaryotes and include the products of a Salmonella enterica serovar Typhimurium gene expressed during invasion of macrophages and psiE, an Escherichia coli phosphate starvation-inducible gene. Although its specific role is undetermined, the presence of ddcA in multicopy restored the seed adhesion capacity of a KT2440 ddcA mutant. Expression of ddcA is growth phase regulated, being maximal at the beginning of stationary phase. It is independent of RpoS, nutrient depletion, or phosphate starvation, and it is not the result of changes in the medium pH during growth. Expression of ddcA is directly dependent on cell density, being also stimulated by the addition of conditioned medium and of seed exudates. This is the first evidence suggesting the existence of a quorum-sensing system in P. putida KT2440. The potential implication of such a signaling process in seed adhesion and colonization by the bacterium is discussed.

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Section: Discussionmentioning

confidence: 99%

Cell Density-Dependent Gene Contributes to Efficient Seed Colonization by Pseudomonas putida KT2440

Espinosa‐Urgel

Ramos

2004

Appl Environ Microbiol

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“…A number of DKPs, including cyclo(L-Tyr-L-Pro) and cyclo(LPhe-L-Pro), have been detected in culture supernatants from a variety of Gram-negative bacteria, such as Pseudomonas spp., Vibrio spp., Proteus mirabilis, Citrobacter freundii, and Enterobacter agglomerans, and they have been shown to modulate quorumsensing systems that commonly respond to acyl-homoserine lactone signaling molecules (26,27,45). Similarly, it was found that Gram-positive Lactobacillus plantarum species also produced cyclic dipeptides, including cyclo(L-Phe-L-Pro), cyclo(L-Phe-trans-4-OH-L-Pro), and cyclo(Gly-L-Leu), which exhibited antifungal activity (28,46).…”

Section: Discussionmentioning

confidence: 99%

Lactobacillus reuteri -produced cyclic dipeptides quench agr -mediated expression of toxic shock syndrome toxin-1 in staphylococci

Wang²,

Xu³

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

193

144

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The production of the staphylococcal exotoxin toxic shock syndrome toxin-1 (TSST-1) by Staphylococcus aureus has been associated with essentially all cases of menstruation-associated toxic shock syndrome (TSS). In this work, we show that the human vaginal isolate Lactobacillus reuteri RC-14 produces small signaling molecules that are able to interfere with the staphylococcal quorum-sensing system agr, a key regulator of virulence genes, and repress the expression of TSST-1 in S. aureus MN8, a prototype of menstrual TSS S. aureus strains. Quantitative real-time PCR data showed that transcription from the Ptst promoter, as well as the P2 and P3 promoters of the agr system from all four agr subgroups of S. aureus, was strongly inhibited in response to growth with L. reuteri RC-14 cultural supernatant. Alterations in the transcriptional levels of two other virulence-associated regulators sarA and saeRS were also observed, indicating a potential overall influence of L. reuteri RC-14 signals on the production of virulence factors in S. aureus. S. aureus promoter-lux reporter strains were used to screen biochemically fractionated L. reuteri RC-14 supernatant, and the cyclic dipeptides cyclo(L-Phe-L-Pro) and cyclo(L-Tyr-L-Pro) were identified as the signaling molecules. The results from this work contribute to a better understanding of interspecies cell-to-cell communication between Lactobacillus and Staphylococcus, and provide a unique mechanism by which endogenous or probiotic strains may attenuate virulence factor production by bacterial pathogens.diketopiperazine | two-component systems

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“…(301) and Gram-negative (302,303) species produce DKPs and appear to be capable of targeting LuxR proteins in engineered biosensors, but they were also found to affect S. liquifaciens swarming (303). The oral pathogen Streptococcus mutans utilizes multiple peptidebased quorum-sensing systems that promote bacteriocin production, competence, and biofilm development.…”

Section: Natural-product Qs Inhibitorsmentioning

confidence: 99%

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

LaSarre

Federle

2013

Microbiol Mol Biol Rev

677

556

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SUMMARY Cell-cell communication, or quorum sensing, is a widespread phenomenon in bacteria that is used to coordinate gene expression among local populations. Its use by bacterial pathogens to regulate genes that promote invasion, defense, and spread has been particularly well documented. With the ongoing emergence of antibiotic-resistant pathogens, there is a current need for development of alternative therapeutic strategies. An antivirulence approach by which quorum sensing is impeded has caught on as a viable means to manipulate bacterial processes, especially pathogenic traits that are harmful to human and animal health and agricultural productivity. The identification and development of chemical compounds and enzymes that facilitate quorum-sensing inhibition (QSI) by targeting signaling molecules, signal biogenesis, or signal detection are reviewed here. Overall, the evidence suggests that QSI therapy may be efficacious against some, but not necessarily all, bacterial pathogens, and several failures and ongoing concerns that may steer future studies in productive directions are discussed. Nevertheless, various QSI successes have rightfully perpetuated excitement surrounding new potential therapies, and this review highlights promising QSI leads in disrupting pathogenesis in both plants and animals.

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Plant Growth-Promoting Pseudomonas putida WCS358 Produces and Secretes Four Cyclic Dipeptides: Cross-Talk with Quorum Sensing Bacterial Sensors

Cited by 173 publications

References 13 publications

Cell Density-Dependent Gene Contributes to Efficient Seed Colonization by Pseudomonas putida KT2440

Cell Density-Dependent Gene Contributes to Efficient Seed Colonization by Pseudomonas putida KT2440

Lactobacillus reuteri -produced cyclic dipeptides quench agr -mediated expression of toxic shock syndrome toxin-1 in staphylococci

Exploiting Quorum Sensing To Confuse Bacterial Pathogens

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