Plant growth promoting potential and phylogenetic characteristics of a lichenized nitrogen fixing bacterium, <i>Enterobacter cloacae</i>

Swamy, Chidanandamurthy Thippeswamy; Gayathri, Devaraja; Devaraja, T. N.; Bandekar, Mandar; D'Souza, Stecy Elvira; Meena, Ramavatar; Ramaiah, N.

doi:10.1002/jobm.201600197

Cited by 24 publications

(13 citation statements)

References 53 publications

(80 reference statements)

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“…We also found P. rettgeri (QS24‐2) as one of the best biofilm‐forming rhizobacteria from the surface of the rice root. Though P. rettgeri is recognized as a pathogen to humans , recently a strain with plant‐growth‐promoting traits was isolated from lichens . However, cell to cell communication via QS in pathogenic strains of Providentia has already been reported .…”

Section: Discussionmentioning

confidence: 99%

Occurrence of diversified N‐acyl homoserine lactone mediated biofilm‐forming bacteria in rice rhizoplane

Balasundararajan

Balachandar

2019

J Basic Microbiol

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Quorum sensing (QS)-mediated biofilm-forming rhizobacteria are indispensable due to their competitiveness in the crop rhizosphere. In the present work, we have reported on the occurrence of diversified bacterial species capable of producing N-acyl homoserine lactone (AHL) as the QS signal in the roots of a rice plant grown under field conditions. The AHL-producing bacteria were directly isolated from the rice root by the biosensor reporter (Chromobacterium violaceum CV026) overlay method and characterized for biofilm production by the microtiter plate method. A total of 48 QS-positive bacterial isolates were purified from different aged (7,20,24,26, and 36 days) rice seedlings. The in vitro biofilm production and genetic diversity as revealed by BOX-PCR fingerprinting showed high variability among the isolates. Most of the best biofilm-forming isolates produced a N-butyryl DL-homoserine lactone (a C4-AHL type) signal in the medium. The 16S ribosomal RNA (rRNA) gene sequence of these putative elite isolates identified that they were close to Aeromonas hydrophila (QS7-4; QS36-2), A. enteropelongenes (QS20-8), A. veronii (QS36-3), Enterobacter sp. (QS20-11), Klebsiella pneumoniae (QS24-6), Kosakonia cowanii (QS24-21), Providentia rettigeri (QS24-2), Sphingomonas aquatilis (QS24-17), and Pseudomonas sihuiensis (QS24-20). These strains profusely colonized the rice root upon inoculation and formed biofilms on the surface of the root under gnotobiotic conditions. Developing inoculants from these strains would ensure competitive colonization on the rhizoplane of the crop through their biofilm and thereby improve plant growth and health. K E Y W O R D S biofilm, N-acyl homoserine lactone, plant-associated bacteria, quorum sensing, rice

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Section: Discussionmentioning

confidence: 99%

Occurrence of diversified N‐acyl homoserine lactone mediated biofilm‐forming bacteria in rice rhizoplane

Balasundararajan

Balachandar

2019

J Basic Microbiol

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“…The nitrogen fixation capacity of strain 1-L-29 was estimated on nitrogen-free bromothymol blue (NFb) medium following Swamy et al [30]; the inorganic phosphorus fixation capacity of this strain was estimated on Pikovskaia's (PKO) medium following Li et al [31]; and the organic phosphorus fixation capacity of this strain was estimated on Mongina organic culture medium following Schwyn and Neilands [32]. The siderophore production of strain 1-L-29 was measured on Chrom-Azurol Siderophore (CAS) agar medium following Rahman et al [33].…”

Section: Physiological Properties Of Strain 1-l-29mentioning

confidence: 99%

Isolation and characterization of Bacillus subtilis strain 1-L-29, an endophytic bacteria from Camellia oleifera with antimicrobial activity and efficient plant-root colonization

et al. 2020

PLoS ONE

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Endophytic bacteria, which are common in plant tissues, may help to control plant pathogens and enhance plant growth. Camellia oleifera, an oil-producing plant, is widely grown in warm, subtropical, hilly regions in China. However, C. oleifera is strongly negatively affected by C. oleifera anthracnose, which is caused by Colletetrichum fructicola. To find a suitable biocontrol agent for C. oleifera anthracnose, 41 endophytes were isolated from the stems, leaves, and roots of C. oleifera. Bacterial cultures were identified based on analyses of 16S rDNA sequences; most strains belonged to the genus Bacillus. The antagonistic effects of these strains on C. fructicola were tested in vitro. In total, 16 strains inhibited C. fructicola growth, with B. subtilis strain 1-L-29 being the most efficient. Strain 1-L-29 demonstrated antagonistic activity against C. siamense, C. asianum, Fusarium proliferatum, Agaricodochium camellia, and Pseudomonas syringae. In addition, this strain produced indole acetic acid, solubilized phosphate, grew on N-free media, and produced siderophores. To facilitate further microecological studies of this strain, a rifampicin-resistant, green fluorescent protein (GFP)-labeled strain, 1-L-29gfp r , was created using protoplast transformation. This plasmid had good segregational stability. Strain 1-L-29gfp r was reintroduced into C. oleifera and successfully colonized root, stem, and leaf tissues. This strain remained at a stable concentration in the root more than 20 d after inoculation. Fluorescence microscopic analysis showed that strain 1-L-29gfp r thoroughly colonized the root surfaces of C. fructicola as well as the root vascular tissues of Arabidopsis thaliana.

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“…The potential fungi were grown on 50 ml of PDB media at 28 ± 2 °C for 5-6 days, and the mycelial mat was harvested, washed thrice using distilled water and ground with liquid nitrogen. Using cetyl trimethyl ammonium bromide (CTAB) method, genomic DNA was extracted and the purity of the DNA was checked spectrophotometrically [19,20].…”

Section: Extraction Of Genomic Dnamentioning

confidence: 99%

Biosorption of heavy metal arsenic from Industrial Sewage of Davangere District, Karnataka, India, using indigenous fungal isolates

Tanvi¹,

Pratam²,

Lohit³

et al. 2020

SN Appl. Sci.

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Contamination of soil and water bodies with heavy metals like arsenic (As) is of major concern, since arsenic is non-biodegradable, toxic and causes ill effects to gastrointestinal system, skin, liver, kidney or neurological functions. In addition, animals affected by arsenic pollution through fodder intern enter to food chain. Hence, there is an urgent need to address heavy metal arsenic pollution especially through bioremediation. In this context, biosorption with indigenous microbiota has gained more interest in recent years as it is cost-effective, eco-friendly approach and with no adverse effects. In the present study, arsenic (As III)-resistant strains of fungi were isolated from soil samples collected from various locations of industrial sewage disposal sites of Davangere District, India, and screened. Among five fungal isolates, Aspergillus spp APR-1 and APR-2 showed greater resistance to arsenic in the laboratory conditions. In order to increase the surface area for biosorption, APR-1 and APR-2 isolates were immobilized on Luffa aegyptiaca (sponge gourd) (an agro-waste as biosorbent). With 250 mM arsenic solution, Aspergillus spp APR-1 and APR-2 showed biosorption of 53.94 and 52.54%, respectively, on inductively coupled plasma-optical emission spectrometry analysis and the adsorption of the fungal isolates on sponge gourd was confirmed using scanning electron microscopy. APR-1 isolate was further characterized by 18 s rDNA typing and identified as Aspergillus niger. Industrial scale application perhaps is taken up using selected fungal strains and mitigates the arsenic pollution in urban sewage.

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Plant growth promoting potential and phylogenetic characteristics of a lichenized nitrogen fixing bacterium, Enterobacter cloacae

Cited by 24 publications

References 53 publications

Occurrence of diversified N‐acyl homoserine lactone mediated biofilm‐forming bacteria in rice rhizoplane

Occurrence of diversified N‐acyl homoserine lactone mediated biofilm‐forming bacteria in rice rhizoplane

Isolation and characterization of Bacillus subtilis strain 1-L-29, an endophytic bacteria from Camellia oleifera with antimicrobial activity and efficient plant-root colonization

Biosorption of heavy metal arsenic from Industrial Sewage of Davangere District, Karnataka, India, using indigenous fungal isolates

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