Plant growth-promoting mechanisms and genetic diversity of bacteria strains isolated from Brachiaria humidicola and Brachiaria decumbens

Oliveira, João Tiago Correia; Pereira, Arthur Prudêncio de Araújo; Souza, Adijailton José de; Silva, Gilka T.; Diniz, Williane P. S.; Figueredo, Everthon Fernandes; Kuklinsky-Sobral, Júlia; Freire, Fernando José

doi:10.1590/0001-3765202120191123

Cited by 4 publications

(3 citation statements)

References 34 publications

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“…For plants, the ALH activity can promote the increase of primary root elongation and growth rate. These effects were mainly attributed to changes in auxin levels (56,60). The Jaccard similarity coefficient revealed the presence of 16 clusters with high genetic variability at 30% similarity.…”

Section: Resultsmentioning

confidence: 98%

Selection and Characterization of Burkholderia spp. for Their Plant-Growth Promoting Effects and Influence on Maize Seed Germination

et al. 2022

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Burkholderia sp. is a bacterial genus extremely versatile in the environment and has been reported for a great potential to promote plant growth via different mechanisms. Here we evaluate the plant growth-promoting mechanisms in twenty-six Burkholderia strains. Strains were evaluated for their ability to promote plant growth by means of: indole-3-acetic acid (IAA) production under different conditions of pH, salt stress and the presence or absence of L-tryptophan; exopolysaccharides (EPS) production and quorum sensing (ALH). The strains were also characterized in terms of their genetic variability and species identification through Sanger sequencing. Then, the bacteria most responsive in the greatest number of plant-growth promotion mechanisms were selected for a corn seed germination test. All bacteria synthesized IAA in medium with 0.0 or 5.0 mM of L-tryptophan in combination with either 1 or 5% of NaCl, and pH values of either 4.5 or 7.2. The EPS production was confirmed for 61.54% of the bacterial strains. Quorum sensing also occurred in 92.3% of the selected bacteria. The Jaccard similarity coefficient revealed 16 clusters with high genetic variability between bacterial strains. Bacterial strains were assigned to seven species: B. anthina, B. cepacia, B. gladioli, B. ambifaria, B. graminis, B. heleia, and Burkholderia spp. The corn seed bacterization did not affect the germination velocity index (GSI), as well as the first count of germinated seeds (FC). However, inoculations formulated with B. heleia strain G28, B. gladioli strain UAGC723, and B. graminis strain UAGC348 promoted significant increases in root length, seedling height and fresh and dry seedling phytomass, respectively. These results indicate the high biotechnological potential of several strains in the genus Burkholderia sp. as seed inoculants, favoring germination and seedling initial development.

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Section: Resultsmentioning

confidence: 98%

Selection and Characterization of Burkholderia spp. for Their Plant-Growth Promoting Effects and Influence on Maize Seed Germination

et al. 2022

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“…These traits occurred in different proportions when other plant species were evaluated. For example, while 48% of the strains grew in N-free media, strains from other grasses grown in the Brazilian tropical semiarid included 66% diazotrophs [ 20 ], and signal grass root and rhizosphere bacteria had 58% diazotrophs [ 21 ].…”

Section: Discussionmentioning

confidence: 99%

Screening Digitaria eriantha cv. Suvernola Endophytic Bacteria for Maize Growth Promotion

Alves

Mendonça

Vitalino

et al. 2023

Plants

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The search for sustainable agriculture has increased interest in using endophytic bacteria to reduce fertilizer use and increase stress resilience. Stress-adapted plants are a potential source of these bacteria. Some species of these plants have not yet been evaluated for this, such as pangolão grass, from which we considered endophytic bacteria as potential plant growth promoters. Bacteria from the root, colm, leaves, and rhizospheric soil were isolated, and 132 strains were evaluated for their in vitro biological nitrogen fixation, IAA and siderophores production, and phosphate solubilization. Each mechanism was also assessed under low N availability, water stress, and low-solubility Fe and P sources in maize greenhouse experiments. All strains synthesized IAA; 63 grew on N-free media, 114 synthesized siderophores, and 46 solubilized P, while 19 presented all four mechanisms. Overall, these strains had better performance than commercial inoculant in all experiments. Still, in vitro responses were not good predictors of in vivo effects, which indicates that the former should not be used for strain selection, since this could lead to not testing strains with good plant growth promotion potential. Their heterologous growth promotion in maize reinforces the potential of stress-adapted plant species as potential sources of strains for inoculants.

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“…The increase in soil porosity can contribute to aerobic activity from bacteria and, especially, fungi communities and consequently increase SOC metabolization (Yang et al 2019). The positive correlation between Brachiaria species and the P availability in the soil is not only due to the association with bacterial communities' groups(Merlin et al 2016;Oliveira et al 2021) but also with AMF.…”

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confidence: 95%

Investigating the effects of Brachiaria (Syn. Urochloa) varieties on soil properties and microbiome

Merloti

Bossolani²,

Mendes³

et al. 2023

Preprint

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Background and Aims The Brachiaria sp. (synonymous with Urochloa) is one of Brazil’s main grass species used in livestock production and has become the focus of breeding genetic programs to enhance its resistance to drought, flooding, and pests, as well as improving its palatability to animals. However, there is a limited understanding of how genetic breeding can affect the soil microbiome and its potential functions. Thus, this study aimed to investigate the impact of four different Brachiaria varieties on the soil prokaryotic and fungal communities, particularly emphasizing their potential functions related to the N-cycle. Methods We combined molecular techniques, such as quantitative PCR and amplicon sequencing, to target prokaryotic and fungi communities and traditional soil and plant chemical analyses. Results Our findings revealed that all varieties improved soil porosity, P content, and organic carbon. Soil acidity, nutrient availability, and porosity were the main drivers of the microbial communities. The Nitrososphaeraceae, Gaiellales, Conexibacter, Sphingomonas, Hydnophlebia meloi, Conocybe, and Cladosporium were the main taxa associated with the dissimilarities between the Brachiaria varieties and the Control. In addition, the presence of the plants increased potential microbial functions such as Chemoheterotroph, Aerobic-Chemoheterotroph, and Pathotroph-Saprotroph groups. The study also identified the ability of each variety to recruit nitrogen-fixing and bacterial and archaeal ammonia-oxidizing communities. Conclusion Our findings suggest that selecting an efficient Brachiaria variety could positively impact soil quality, improving agricultural systems and increasing food production.

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Plant growth-promoting mechanisms and genetic diversity of bacteria strains isolated from Brachiaria humidicola and Brachiaria decumbens

Cited by 4 publications

References 34 publications

Selection and Characterization of Burkholderia spp. for Their Plant-Growth Promoting Effects and Influence on Maize Seed Germination

Selection and Characterization of Burkholderia spp. for Their Plant-Growth Promoting Effects and Influence on Maize Seed Germination

Screening Digitaria eriantha cv. Suvernola Endophytic Bacteria for Maize Growth Promotion

Investigating the effects of Brachiaria (Syn. Urochloa) varieties on soil properties and microbiome

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