Plant-growth promoting effect of newly isolated rhizobacteria varies between two Arabidopsis ecotypes

Schwachtje, Jens; Karojet, Silke; Kunz, Sabine; Brouwer, Stephan; Dongen, Joost Thomas van

doi:10.4161/psb.20176

Cited by 22 publications

(16 citation statements)

References 37 publications

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“…1 , representative plantlets subjected to BsuGB03 volatiles reached the 3-leaf stage (stage 13, [ 40 ]) after 10 days of co-cultivation, whereas control plantlets had only two unfolded leaves (stage 12). This observation is consistent with the results reported by [ 41 ], indicating that PGPR can induce significant changes in plant growth rate. This could also explain the observed RSA differences because B. distachyon plantlets are known to produce up to two coleoptile nodal adventitious roots at stage 13 [ 39 ].…”

Section: Discussionsupporting

confidence: 94%

Influence of rhizobacterial volatiles on the root system architecture and the production and allocation of biomass in the model grass Brachypodium distachyon (L.) P. Beauv.

et al. 2015

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BackgroundPlant growth-promoting rhizobacteria are increasingly being seen as a way of complementing conventional inputs in agricultural systems. The effects on their host plants are diverse and include volatile-mediated growth enhancement. This study sought to assess the effects of bacterial volatiles on the biomass production and root system architecture of the model grass Brachypodium distachyon (L.) Beauv.ResultsAn in vitro experiment allowing plant-bacteria interaction throughout the gaseous phase without any physical contact was used to screen 19 bacterial strains for their growth-promotion ability over a 10-day co-cultivation period. Five groups of bacteria were defined and characterised based on their combined influence on biomass production and root system architecture. The observed effects ranged from unchanged to greatly increased biomass production coupled with increased root length and branching. Primary root length was increased only by the volatile compounds emitted by Enterobacter cloacae JM22 and Bacillus pumilus T4. Overall, the most significant results were obtained with Bacillus subtilis GB03, which induced an 81 % increase in total biomass, as well as enhancing total root length, total secondary root length and total adventitious root length by 88.5, 201.5 and 474.5 %, respectively.ConclusionsThis study is the first report on bacterial volatile-mediated growth promotion of a grass plant. Contrasting modulations of biomass production coupled with changes in root system architecture were observed. Most of the strains that increased total plant biomass also modulated adventitious root growth. Under our screening conditions, total biomass production was strongly correlated with the length and branching of the root system components, except for primary root length. An analysis of the emission kinetics of the bacterial volatile compounds is being undertaken and should lead to the identification of the compounds responsible for the observed growth-promotion effects. Within the context of the inherent characteristics of our in vitro system, this paper identifies the next critical experimental steps and discusses them from both a fundamental and an applied perspective.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0585-3) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 94%

Influence of rhizobacterial volatiles on the root system architecture and the production and allocation of biomass in the model grass Brachypodium distachyon (L.) P. Beauv.

et al. 2015

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“…While there is no evidence that the sequenced strains of P. brassicacearum are phytotoxic, this could be representative of a bias towards an interest in P. brassicacearum with PGP properties, or a lack of testing on susceptible hosts. A few phytopathogenic isolates of P. brassicacearum that also exhibit PGP or biocontrol characteristics have been described [122][123][124][125]. It has been suggested that these P. brassicacearum are 'minor' , 'quasi' , or 'opportunistic' phytopathogens rather than true disease-causing organisms [12,76,122].…”

Section: Pseudomonas Brassicacearum-plant Associations: Duality In Namentioning

confidence: 99%

Friend or foe? Exploring the fine line between Pseudomonas brassicacearum and phytopathogens

Gislason

Kievit

2020

Journal of Medical Microbiology

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Pseudomonas brassicacearum is one of over fifty species of bacteria classified into the P. fluorescens group. Generally considered a harmless commensal, these bacteria are studied for their plant-growth promotion (PGP) and biocontrol characteristics. Intriguingly, P. brassicacearum is closely related to P. corrugata , which is classified as an opportunistic phytopathogen. Twenty-one P. brassicacearum genomes have been sequenced to date. In the current review, genomes of P. brassicacearum and strains from the P. corrugata clade were mined for regions associated with PGP, biocontrol and pathogenicity. We discovered that ‘beneficial’ bacteria and those classified as plant pathogens have many genes in common; thus, only a fine line separates beneficial/harmless commensals from those capable of causing disease in plants. The genotype and physiological state of the plant, the presence of biotic/abiotic stressors, and the ability of bacteria to manipulate the plant immune system collectively contribute to how the bacterial-plant interaction plays out. Because production of extracellular metabolites is energetically costly, these compounds are expected to impart a fitness advantage to the producer. P. brassicacearum is able to reduce the threat of nematode predation through release of metabolites involved in biocontrol. Moreover this bacterium has the unique ability to form biofilms on the head of Caenorhabditis elegans, as a second mechanism of predator avoidance. Rhizobacteria, plants, fungi, and microfaunal predators have occupied a shared niche for millions of years and, in many ways, they function as a single organism. Accordingly, it is essential that we appreciate the dynamic interplay among these members of the community.

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“…For example, Pseudomonas fluorescens strain WCS417 promotes growth mediated by IAA production and ACC deaminase activity, and ISR via jasmonic acid signalling (Schwachtje et al . ; Zamioudis et al . ).…”

Section: Impacts On Plant Performancementioning

confidence: 99%

“…Some rhizobacteria are capable of both plant growth-promoting activity and ISR induction. For example, Pseudomonas fluorescens strain WCS417 promotes growth mediated by IAA production and ACC deaminase activity, and ISR via jasmonic acid signalling (Schwachtje et al 2012;Zamioudis et al 2013).…”

Section: Impacts On Plant Performancementioning

confidence: 99%

Giving back to the community: microbial mechanisms of plant–soil interactions

Paredes

Lebeis

2016

Functional Ecology

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Summary1. The role of both plants and soil microbes on ecosystem functioning has been long recognized, but the precise feedback mechanisms between them are more elusive. Definition of these interactions is critical if we aim to achieve an integral understanding of ecosystem functioning, and ultimately explain natural, agricultural and synthetic systems. 2. Advances in genomic technologies and the development of more appropriate statistical, mathematical and computational frameworks enable researchers to almost fully describe and measure the diversity of microbial communities in soil, rhizosphere and plant tissues. Under the scaffold of community ecology, we integrate the observed patterns of microbial diversity with current mechanistic understanding of plant-microbe mutualistic and pathogenic interactions, and propose a model in which plant microbial communities are shaped by different ecological forces differentially through the plant life cycle. 3. The same genomic technologies, applied on natural and reconstructed systems, establish that plant genotype has a small, but significant, effect on the microbial community composition in, on and around plant organs. Despite these advances, technical limitations are still important and only a handful of studies exist where a precise genetic element definitively participates in these interactions. 4. Studies at the field or ecosystem level are dominated by agricultural settings, examining microbial species and communities effects on plant productivity; and conversely, that plant genetics and agricultural practices can potentially impose selective pressures on specific microbes and microbial communities. 5. Revitalized interest in plant-soil microbial feedbacks requires researchers to systematically pose and evaluate more complex hypotheses with increasingly more realistic microbial settings. Despite the advances reviewed here, most studies focus on one aspect of plant, microbe and soil interactions. Experiments that simultaneously and methodically manipulate multiple components are necessary to establish the ecological principles, and molecular mechanisms, which drive microbially mediated plant-soil interactions. This knowledge will be critical to predict how environmental changes affect microbial and plant diversity, and will guide efforts to improve agricultural and conservation practices.

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Plant-growth promoting effect of newly isolated rhizobacteria varies between two Arabidopsis ecotypes

Cited by 22 publications

References 37 publications

Influence of rhizobacterial volatiles on the root system architecture and the production and allocation of biomass in the model grass Brachypodium distachyon (L.) P. Beauv.

Influence of rhizobacterial volatiles on the root system architecture and the production and allocation of biomass in the model grass Brachypodium distachyon (L.) P. Beauv.

Friend or foe? Exploring the fine line between Pseudomonas brassicacearum and phytopathogens

Giving back to the community: microbial mechanisms of plant–soil interactions

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