“…S1 – S3 ). The BI-PCG and ML-PCG analyses align more closely with previous phylogenetic studies 41 , 53 . Figure 6 Phylogenetic relationships of Pleosporales inferred by MrBayes 3.2.6 based on the nucleotides of the first and second codons of the 12 protein-coding genes (11,688 bp) and two rRNAs.…”
Endophytic fungi play an important role in the growth and development of traditional Chinese medicine plants. We isolated a strain of Acrocalymma vagum from the endophytic fungi of the traditional Chinese plants Paris. To accurately identify this endophytic fungal species of interest, we sequenced the mitochondrial genome of A. vagum, which is the first discovered mitochondrial genome in Massarineae. The A. vagum mitochondrial genome consists of a 35,079-bp closed circular DNA molecule containing 36 genes. Then, we compared the general sequence characteristics of A. vagum with those of Pleosporales, and the second structure of the 22 tRNAs was predicted. The phylogenetic relationship of A. vagum was constructed using two different data sets (protein-coding genes and amino acids). The phylogenetic tree shows that A. vagum is located at the root of Pleosporales. The analysis of introns shows that the number of introns increases with the increase in branch length. The results showed that monophyly was confirmed for all families in Pleosporales except for Pleosporaceae. A. vagum is an ancient species in the Pleosporales, and Pleosporaceae may require further revision. In Pleosporales, the number of introns is positively correlated with branch length, providing data for further study on the origin of introns.
“…S1 – S3 ). The BI-PCG and ML-PCG analyses align more closely with previous phylogenetic studies 41 , 53 . Figure 6 Phylogenetic relationships of Pleosporales inferred by MrBayes 3.2.6 based on the nucleotides of the first and second codons of the 12 protein-coding genes (11,688 bp) and two rRNAs.…”
Endophytic fungi play an important role in the growth and development of traditional Chinese medicine plants. We isolated a strain of Acrocalymma vagum from the endophytic fungi of the traditional Chinese plants Paris. To accurately identify this endophytic fungal species of interest, we sequenced the mitochondrial genome of A. vagum, which is the first discovered mitochondrial genome in Massarineae. The A. vagum mitochondrial genome consists of a 35,079-bp closed circular DNA molecule containing 36 genes. Then, we compared the general sequence characteristics of A. vagum with those of Pleosporales, and the second structure of the 22 tRNAs was predicted. The phylogenetic relationship of A. vagum was constructed using two different data sets (protein-coding genes and amino acids). The phylogenetic tree shows that A. vagum is located at the root of Pleosporales. The analysis of introns shows that the number of introns increases with the increase in branch length. The results showed that monophyly was confirmed for all families in Pleosporales except for Pleosporaceae. A. vagum is an ancient species in the Pleosporales, and Pleosporaceae may require further revision. In Pleosporales, the number of introns is positively correlated with branch length, providing data for further study on the origin of introns.
“…Gene specific primers for P. polyphylla var . yunnanensis steroidal saponins biosynthesis genes including 3-hydroxy-3-methylglutaryl-CoA reductase ( HMGS ), geranyl pyrophosphate synthase ( GPPS ), farnesyl pyrophosphate synthase ( FPPS ), squalene synthase ( SS ), squalene epoxidase 1 ( SE1 ), squalene epoxidase 2 ( SE2 ) and cycloartenol synthase ( CAS ) used for qRT-PCR were shown in Liu et al, 2022 . These primers are designed based on our transcriptome data of P. polyphylla var .…”
“…The use of chemical pesticides cause serious pollution to the environment and even endangers human health [123], so it is necessary to put forward more sustainable development strategies for the environment and human health and find suitable pesticide substitutes. Endophytes come from plants and act on plants, and they are rich in species and have strong biological control functions [67,124]. Therefore, they are the most suitable to replace chemical pesticides in the cultivation and protection of medicinal plants, and are an important environmental protection strategy.…”
With the increasing demand for medicinal plants and the increasing shortage of resources, improving the quality and yield of medicinal plants and making more effective use of medicinal plants has become an urgent problem to be solved. In the process of growth and development of medicinal plants, due to the threat of various adversity, there are problems such as nutrient loss and yield decline. Using traditional chemical pesticides to control the stress resistance of plants will cause serious pollution to the environment and even endanger human health. Therefore, it is necessary to find suitable pesticide substitutes from natural ingredients. As an important part of the microecology of medicinal plants, endophytes can promote the growth of medicinal plants, improve the stress resistance of hosts, and promote the accumulation of active components of hosts. Because it has a more positive and direct impact on the host, and can metabolize rich medicinal ingredients, so researchers pay attention to it. This paper reviews the research in recent five years, aiming to provide ideas for improving the quality of medicinal plants, developing more microbial resources, exploring more medicinal natural products, and providing help for the development of the research on medicinal plants and endophytes.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
