Plant growth-promoting activities and genomic analysis of the stress-resistant Bacillus megaterium STB1, a bacterium of agricultural and biotechnological interest

Nascimento, Francisco X.; Hernández, Anabel González; Glick, Bernard R.; Rossi, Márcio José

doi:10.1016/j.btre.2019.e00406

Cited by 102 publications

(87 citation statements)

References 74 publications

(83 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Polyamines (PA) have been also correlated with lateral root, ethylene modulation and oxidative stress alleviation 21,64 . Considering the role of spermidine in bacteria biofilm formation 65 , genes involved in PA pathways may constitute an additional mechanism related to PGP properties, as suggested in Serratia marcescens 21 or Bacillus megaterium STB1 66 . The majority of the selected PGPR possessed PA-related genes, except for SAVSo04 whose genome contained the ipdc gene.…”

Section: Discussionmentioning

confidence: 99%

Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits

Leontidou

Genitsaris

Παπαδοπούλου

et al. 2020

Sci Rep

123

View full text Add to dashboard Cite

Plant growth promoting rhizobacteria (PGPR) are able to provide cross-protection against multiple stress factors and facilitate growth of their plant symbionts in many ways. The aim of this study was to isolate and characterize rhizobacterial strains under natural conditions, associated with naturally occurring representatives of wild plant species and a local tomato cultivar, growing in differently stressed Mediterranean ecosystems. A total of 85 morphologically different rhizospheric strains were isolated; twenty-five exhibited multiple in vitro PGP-associated traits, including phosphate solubilization, indole-3-acetic acid production, and 1-aminocyclopropane-1-carboxylate deaminase activity. Whole genome analysis was applied to eight selected strains for their PGP potential and assigned seven strains to Gammaproteobacteria, and one to Bacteroidetes. The genomes harboured numerous genes involved in plant growth promotion and stress regulation. They also support the notion that the presence of gene clusters with potential PGP functions is affirmative but not necessary for a strain to promote plant growth under abiotic stress conditions. The selected strains were further tested for their ability to stimulate growth under stress. This initial screening led to the identification of some strains as potential PGPR for increasing crop production in a sustainable manner.

show abstract

Section: Discussionmentioning

confidence: 99%

Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits

Leontidou

Genitsaris

Παπαδοπούλου

et al. 2020

Sci Rep

123

View full text Add to dashboard Cite

show abstract

“…The genome annotation was performed using the NCBI Prokaryotic annotation pipeline (Angiuoli et al ., ). Functional genome annotation was performed as described by Nascimento and colleagues ().…”

Section: Methodsmentioning

confidence: 99%

The extreme plant‐growth‐promoting properties ofPantoea phytobeneficialisMSR2 revealed by functional and genomic analysis

Nascimento

Hernández

Glick

et al. 2020

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Summary Numerous Pantoea strains are important because of the benefit they provide in the facilitation of plant growth. However, Pantoea have a high level of genotypic diversity and not much is understood regarding their ability to function in a plant beneficial manner. In the work reported here, the plant growth promotion activities and the genomic properties of the unusual Pantoea phytobeneficialis MSR2 are elaborated, emphasizing the genetic mechanisms involved in plant colonization and growth promotion. Detailed analysis revealed that strain MSR2 belongs to a rare group of Pantoea strains possessing an astonishing number of plant growth promotion genes, including those involved in nitrogen fixation, phosphate solubilization, 1‐aminocyclopropane‐1‐carboxylic acid deaminase activity, indoleacetic acid and cytokinin biosynthesis, and jasmonic acid metabolism. Moreover, the genome of this bacterium also contains genes involved in the metabolism of lignin and other plant cell wall compounds, quorum‐sensing mechanisms, metabolism of plant root exudates, bacterial attachment to plant surfaces and resistance to plant defences. Importantly, the analysis revealed that most of these genes are present on accessory plasmids that are found within a small subset of Pantoea genomes, reinforcing the idea that Pantoea evolution is largely mediated by plasmids, providing new insights into the evolution of beneficial plant‐associated Pantoea.

show abstract

“…According to El-Komy, (2005), wheat inoculated with B. megaterium exhibited higher shoot dry weight, total nitrogen yield and phosphate contents. Studies by Nascimento et al (2020) and Wu et al (2019) show B. megaterium possess genetic elements that play a role in xenobiotic degradation, stress resistance, pathogen antagonistic activities, and other soil rhizosphere colonization traits. Five isolates were closely related to B. simplex, a bacterium species reported by Schwartz et al (2013) to modify the architecture of the roots by enhancing the formation of more lateral roots in pea plants.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Phosphate Solubilizing and Nitrogen-Fixing Bacteria from Lake Ol’Bolossat Sediments, Kenya

Wafula¹,

Murunga²

2020

MAS

View full text Add to dashboard Cite

Phosphate solubilizing and nitrogen-fixing bacteria have a vital role in improving soil fertility and reverting adversely affected soil properties. These bacteria could contribute towards sustainable agriculture with a focus on reducing excessive use of commercial fertilizers. This study aimed at investigating autochthonous populations of phosphate solubilizing and nitrogen-fixing bacteria from Lake Ol’Bolossat sediments. The total microbial counts ranged between 4.8 x 103 to 8.5 x 105 cfu/ml. A total of 50 bacteria were isolated, 34 were obtained from Pikovskaya’s agar medium while 16 were obtained from Norris Glucose Nitrogen free medium. Based on morphological and 16S rRNA gene analyses, the isolates were clustered under the genera Bacillus, Arthrobacter, Pseudomonas, Paenibacillus, Fictibacillus and Acinetobacter. Among potentially novel strains, four strains NFDA2, PKGBC1 (MT799539), PKGB5 and SCEC2 (MT799543) belonged to genus Bacillus, three strains NFGA1 (MT799529), NFGA4 and SCDB3 belonged to the genus Pseudomonas, two strains NFEB6 (MT799528) and NFDC5 belonged to the genus Paenibacillus, one strain PKHC3 (MT7995441) belonged to the genus Arthrobacter while one strain NFDC4, belonged to the genus Acinetobacter. Generally, the phosphate solubilizing bacteria were the most diverse and genera Bacillus, Fictibacillus and Pseudomonas were the most dominant, however, nitrogen-fixing bacteria were dominated by genera Arthrobacter and Pseudomonas.

show abstract

Plant growth-promoting activities and genomic analysis of the stress-resistant Bacillus megaterium STB1, a bacterium of agricultural and biotechnological interest

Cited by 102 publications

References 74 publications

Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits

Plant growth promoting rhizobacteria isolated from halophytes and drought-tolerant plants: genomic characterisation and exploration of phyto-beneficial traits

The extreme plant‐growth‐promoting properties ofPantoea phytobeneficialisMSR2 revealed by functional and genomic analysis

Isolation and Identification of Phosphate Solubilizing and Nitrogen-Fixing Bacteria from Lake Ol’Bolossat Sediments, Kenya

Contact Info

Product

Resources

About