Plant growth inhibitory activity of fatty acids and the related compounds by the Avena coleoptile test

Ohkawa, Makio; Nishikawa, Yoshihiro

doi:10.1016/0168-9452(87)90175-0

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…Thus, it took a lower level of FFAs in OE than the wild type or KO to reach the same threshold of inhibition of growth. FFAs have been reported as inhibitors of tobacco (Nicotiana tabacum) auxillary bud growth (Tso, 1964), buckwheat (Fagopyrum esculentum) seedling growth (Tsuzuki et al, 1987), straight growth of the Avena coleoptile (Ohkawa and Nishikawa, 1987), and growth of the microalgae Monodus subterraneus (Bosma et al, 2008). In addition, lysophospholipids also inhibit cell and root elongation.…”

Section: Discussionmentioning

confidence: 99%

Patatin-Related Phospholipase pPLAIIIβ-Induced Changes in Lipid Metabolism Alter Cellulose Content and Cell Elongation in Arabidopsis

et al. 2011

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The release of fatty acids from membrane lipids has been implicated in various plant processes, and the patatin-related phospholipases (pPLAs) constitute a major enzyme family that catalyzes fatty acid release. The Arabidopsis thaliana pPLA family has 10 members that are classified into three groups. Group 3 pPLAIII has four members but lacks the canonical lipase/esterase consensus catalytic sequences, and their enzymatic activity and cellular functions have not been delineated. Here, we show that pPLAIIIb hydrolyzes phospholipids and galactolipids and additionally has acyl-CoA thioesterase activity. Alterations of pPLAIIIb result in changes in lipid levels and composition. pPLAIIIb-KO plants have longer leaves, petioles, hypocotyls, primary roots, and root hairs than wild-type plants, whereas pPLAIIIb-OE plants exhibit the opposite phenotype. In addition, pPLAIIIb-OE plants have significantly lower cellulose content and mechanical strength than wild-type plants. Root growth of pPLAIIIb-KO plants is less sensitive to treatment with free fatty acids, the enzymatic products of pPLAIIIb, than wild-type plants; root growth of pPLAIIIb-OE plants is more sensitive. These data suggest that alteration of pPLAIIIb expression and the resulting lipid changes alter cellulose content and cell elongation in Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

Patatin-Related Phospholipase pPLAIIIβ-Induced Changes in Lipid Metabolism Alter Cellulose Content and Cell Elongation in Arabidopsis

et al. 2011

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“…Thus, overaccumulation of free FAs is expected to be detrimental to tissues. For example, exogenous and endogenous FAs are known to inhibit coleoptile elongation of oat (Avena fatua; Ando and Tsukamotoa, 1981;Ohkawa and Nishikawa, 1987), longitudinal cell growth of Arabidopsis (Li et al, 2011), axillary bud growth of tobacco (Tso, 1964), seedling growth of rice (Oryza sativa; Tsuzuki et al, 1987), and germination of lettuce (Lactuca sativa), oat, and mustard (Sinapsis alba; Le Poidevin, 1965;Stewart and Berrie, 1979;Metzger and Sebesta, 1982). Furthermore, the accumulation of unsaturated FAs has been linked to cell death.…”

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confidence: 99%

Ectopic expression of WRI1 affects fatty acid homeostasis in Brachypodium distachyon vegetative tissues

et al. 2015

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Triacylglycerol (TAG) is a storage lipid used for food purposes and as a renewable feedstock for biodiesel production. WRINKLED1 (WRI1) is a transcription factor that governs fatty acid (FA) synthesis and, indirectly, TAG accumulation in oil-storing plant tissues, and its ectopic expression has led to TAG accumulation in vegetative tissues of different dicotyledonous plants. The ectopic expression of BdWRI1 in the grass Brachypodium distachyon induced the transcription of predicted genes involved in glycolysis and FA biosynthesis, and TAG content was increased up to 32.5-fold in 8-week-old leaf blades. However, the ectopic expression of BdWRI1 also caused cell death in leaves, which has not been observed previously in dicotyledonous plants such as Arabidopsis (Arabidopsis thaliana). Lipid analysis indicated that the free FA content was 2-fold elevated in BdWRI1-expressing leaf blades of B. distachyon. The transcription of predicted genes involved in b-oxidation was induced. In addition, linoleic FA treatment caused cell death in B. distachyon leaf blades, an effect that was reversed by the addition of the FA biosynthesis inhibitor cerulenin. Taken together, ectopic expression of BdWRI1 in B. distachyon enhances FA biosynthesis and TAG accumulation in leaves, as expected, but also leads to increased free FA content, which has cytotoxic effects leading to cell death. Thus, while WRI appears to ubiquitously affect FA biosynthesis and TAG accumulation in diverse plants, its ectopic expression can lead to undesired side effects depending on the context of the specific lipid metabolism of the respective plant species.

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Plant Growth Regulatory Substances Both of Microbial and Plant Origin

Gross

1991

Chemistry of Plant Protection

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Plant growth inhibitory activity of fatty acids and the related compounds by the Avena coleoptile test

Cited by 5 publications

References 23 publications

Patatin-Related Phospholipase pPLAIIIβ-Induced Changes in Lipid Metabolism Alter Cellulose Content and Cell Elongation in Arabidopsis

Patatin-Related Phospholipase pPLAIIIβ-Induced Changes in Lipid Metabolism Alter Cellulose Content and Cell Elongation in Arabidopsis

Ectopic expression of WRI1 affects fatty acid homeostasis in Brachypodium distachyon vegetative tissues

Plant Growth Regulatory Substances Both of Microbial and Plant Origin

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