Plant Glutathione Transferases in Abiotic Stress Response and Herbicide Resistance

Chronopoulou, Evangelia; Georgakis, Nikolaos; Nianiou-Obeidat, Irini; Madesis, Panagiotis; Perperopoulou, Fereniki; Pouliou, Fotini; Ioannou, Elisavet; Ataya, Farid S.; Labrou, Nikolaos E.

doi:10.1007/978-3-319-66682-2_10

Cited by 29 publications

(23 citation statements)

References 107 publications

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“…Zhang et al [3] reported that increased activity of GST in maize leaf under low P is likely to protect maize from damage caused by abiotic stresses through scavenging ROS. However, to our best knowledge, this is the first report to change GST activity by Tre in low P stress in the plant [58].…”

Section: Discussionmentioning

confidence: 72%

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Rohman

Islam

Monsur

et al. 2019

Plants

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This study is undertaken to elucidate the role of trehalose (Tre) in mitigating oxidative stress under salinity and low P in maize. Eight-day-old maize seedlings of two maize varieties, BARI Hybrid Maize-7 and BARI Hybrid Maize-9, were subjected to salinity (150 mM NaCl), low P (5 µM KH2PO4) and their combined stress with or without 10 mM Tre for 15 d. Salinity and combined stress significantly inhibited the shoot length, root length, and root volume, whereas low P increased the root length and volume in both genotypes. Exogenous Tre in the stress treatments increased all of the growth parameters as well as decreased the salinity, low P, and combined stress-mediated Na+/K+, reactive oxygen species (ROS), malondialdehyde (MDA), lipoxygenase (LOX) activity, and methylglyoxal (MG) in both genotypes. Individually, salinity and low P increased superoxide dismutase (SOD) activity in both genotypes, but combined stress decreased the activity. Peroxidase (POD) activity increased in all stress treatments. Interestingly, Tre application enhanced the SOD activity in all the stress treatments but inhibited the POD activity. Both catalase (CAT) and glutathione peroxidase (GPX) activity were increased by saline and low P stress while the activities inhibited in combined stress. Similar results were found for ascorbate peroxidase (APX), glutathione peroxidase (GR), and dehydroascorbate reductase (DHAR) activities in both genotypes. However, monodehydroascorbate reductase (MDHAR) activity was inhibited in all the stresses. Interestingly, Tre enhanced CAT, APX, GPX, GR, MDHAR, and DHAR activities suggesting the amelioration of ROS scavenging in maize under all the stresses. Conversely, increased glyoxalase activities in saline and low P stress in BHM-9 suggested better MG detoxification system because of the down-regulation of glyoxalase-I (Gly-I) activity in BHM-7 in those stresses. Tre also increased the glyoxalase activities in both genotypes under all the stresses. Tre improved the growth in maize seedlings by decreasing Na+/K+, ROS, MDA, and MG through regulating antioxidant and glyoxalase systems.

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Section: Discussionmentioning

confidence: 72%

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Rohman

Islam

Monsur

et al. 2019

Plants

View full text Add to dashboard Cite

show abstract

“…Zhang et al [3] reported increased activity of GST in maize leaf under low P to likely protect maize from damage caused by abiotic stresses through scavenging ROS. However, to our best knowledge, this is the first report to change GST activity by Tre in low P stress in the plant [58].…”

Section: Discussionmentioning

confidence: 72%

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Rohman¹,

Islam²,

Monsur³

et al. 2019

Preprint

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This study was undertaken to elucidate the role of trehalose (Tre) in mitigating oxidative stress under salinity and low P in maize. Eight-day-old maize seedlings of two maize varieties, BARI Hybrid Maize-7 and BARI Hybrid Maize-9 were subjected to salinity (150 mM NaCl), low P (5 µM KH2PO4) and their combined stress with or without 10 mM Tre for 15-d.Salinity and combined stress significantly inhibited the shoot length, root length, and root volume, whereas, low P increased the root length and volume in both genotypes. Exogenous Tre in the stress treatments increased all of the growth parameters as well as decreased the salinity, low P and combined stress-mediated Na+/K+, ROS, MDA, LOX activity and MG in both genotypes. Under salinity and low P stress, the SOD activity increased in both genotypes, but the activity decreased in combined stress. POD activity increased in all stress treatments. Interestingly, Tre application enhanced the SOD activity in all the stress treatments but inhibited the POD activity. Both CAT and GPX activity were increased by saline and low P stress while the activities inhibited in combined stress. Similar results were found for APX, GR, and DHAR activities in both genotypes. However, MDHAR activity was inhibited in all the stresses. Interestingly, Tre enhanced CAT APX, GPX, GR, MDHAR and DHAR activities suggesting the amelioration of ROS scavenging in maize under all the stresses. Increased GST activity in presence or absence of Tre might involve in detoxification of hydroperoxides as well as leaf senescence. On the other hand, increased glyoxalase activities in saline and low P stress in BHM-9 suggested better MG detoxification system because of down-regulation of Gly-I activity in BHM-7 in those stresses. Tre also increased the glyoxalase activities in both genotypes under all the stresses. Tre improved the growth in maize seedlings by decreasing Na+/K+, ROS, MDA, and MG through regulating antioxidant and glyoxalase systems.

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“…The TIBA application to plants also induces glutathione S-transferases (GSTs) (Flury et al 1995, 1996). The increased level of GSTs can reduce intracellular GSH concentration by its conjugation to endogenous electrophilic compounds (Chronopoulou et al 2017). The microarray studies indicated that GST transcripts are underexpressed in the roots and hypocotyl of pct1-2 mutant.…”

Section: Discussionmentioning

confidence: 99%

Enhanced polar auxin transport in tomato polycotyledon mutant seems to be related to glutathione levels

Nongmaithem

Ponukumatla

Sreelakshmi

et al. 2019

Preprint

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AbstractGlutathione-dependent root growth in Arabidopsis is linked to polar auxin transport (PAT). Arabidopsis mutants with reduced glutathione (GSH) levels also show reduced PAT. To gain an insight into the relationship between PAT and GSH level, we analysed tomato polycotyledon mutant, pct1-2, which has enhanced PAT. Microarray analysis of gene expression in pct1-2 mutant revealed underexpression of several genes related to glutamate and glutathione metabolism. In consonance with microarray analysis, enzymatic as well as in-vivo assay revealed higher glutathione level in the early phase of pct1-2 seedling growth than WT. The inhibition of auxin transport by 2,3,5-triiodobenzoic acid (TIBA) reduced both GSH level and PIN1 expression in pct1-2 root tips. The reduction of in vivo GSH accumulation in pct1-2 root tips by buthionine sulfoximine (BSO) stimulated elongation of the short root of pct1-2 mutant akin to TIBA. The rescue of short root phenotype of pct1-2 mutant was restricted to TIBA and BSO. The other auxin transport inhibitors 1-N-naphthylphthalamic acid (NPA), 2-[4-(diethylamino)-2-hydroxybenzoyl] benzoic acid (BUM), 3-chloro-4-hydroxyphenylacetic acid (CHPAA), brefeldin and gravacin inhibited root elongation in both WT and pct1-2 mutant. Our results indicate a relationship between PAT and GSH level in tomato akin to Arabidopsis. Our work also highlights that TIBA rescues short root phenotype of the pct1-2 mutant by acting on a PAT component distinct from the site of action of other PAT inhibitors.

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Plant Glutathione Transferases in Abiotic Stress Response and Herbicide Resistance

Cited by 29 publications

References 107 publications

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Trehalose Protects Maize Plants from Salt Stress and Phosphorus Deficiency

Enhanced polar auxin transport in tomato polycotyledon mutant seems to be related to glutathione levels

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