Plant genome engineering from lab to field—a Keystone Symposia report

Cable, Jennifer; Ronald, Pamela C.; Voytas, Daniel F.; Zhang, Feng; Levy, Avraham A.; Takatsuka, Ayumu; Arimura, Shin‐ichi; Jacobsen, Steven E.; Toki, Seiichi; Toda, Erika; Gao, Caixia; Zhu, Jian‐Kang; Boch, Jens; Eck, Joyce Van; Mahfouz, Magdy M.; Andersson, Mariette; Fridman, Eyal; Weiss, Trevor; Wang, Kan; Qi, Yiping; Jores, Tobias; Adams, Tom; Bagchi, Rammyani

doi:10.1111/nyas.14675

Cited by 5 publications

(1 citation statement)

References 112 publications

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“… Ghoshal et al (2021) showed that with epigenome engineering it is possible to achieve heritable methylation, gene silencing, and delayed flowering time phenotypes. Hence, stable heritable epigenetic modifications in flowering time would allow researchers and breeders to overcome the crossing barriers of the secondary and tertiary genepool, facilitating crossings of CWRs with related crops ( Cable et al, 2021 ). Genome editing can also be used to knock out genes.…”

Section: De Novo Domestication and Genome Editingmentioning

confidence: 99%

How Could the Use of Crop Wild Relatives in Breeding Increase the Adaptation of Crops to Marginal Environments?

et al. 2022

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Alongside the use of fertilizer and chemical control of weeds, pests, and diseases modern breeding has been very successful in generating cultivars that have increased agricultural production several fold in favorable environments. These typically homogeneous cultivars (either homozygous inbreds or hybrids derived from inbred parents) are bred under optimal field conditions and perform well when there is sufficient water and nutrients. However, such optimal conditions are rare globally; indeed, a large proportion of arable land could be considered marginal for agricultural production. Marginal agricultural land typically has poor fertility and/or shallow soil depth, is subject to soil erosion, and often occurs in semi-arid or saline environments. Moreover, these marginal environments are expected to expand with ongoing climate change and progressive degradation of soil and water resources globally. Crop wild relatives (CWRs), most often used in breeding as sources of biotic resistance, often also possess traits adapting them to marginal environments. Wild progenitors have been selected over the course of their evolutionary history to maintain their fitness under a diverse range of stresses. Conversely, modern breeding for broad adaptation has reduced genetic diversity and increased genetic vulnerability to biotic and abiotic challenges. There is potential to exploit genetic heterogeneity, as opposed to genetic uniformity, in breeding for the utilization of marginal lands. This review discusses the adaptive traits that could improve the performance of cultivars in marginal environments and breeding strategies to deploy them.

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Section: De Novo Domestication and Genome Editingmentioning

confidence: 99%

How Could the Use of Crop Wild Relatives in Breeding Increase the Adaptation of Crops to Marginal Environments?

et al. 2022

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Harnessing the potential of CRISPR/Cas system for enhancing virus resistance in plants: Targets, strategies, and challenges

Jeyaraj,

Alphonse,

Jayanthi

et al. 2024

Physiological and Molecular Plant Pathology

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Establishment of genome‐editing system and assembly of a near‐complete genome in broomcorn millet

Liu,

Cheng,

Chen

et al. 2024

JIPB

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The ancient crop broomcorn millet (Panicum miliaceum L.) is an indispensable orphan crop in semi‐arid regions due to its short life cycle and excellent abiotic stress tolerance. These advantages make it an important alternative crop to increase food security and achieve the goal of zero hunger, particularly in light of the uncertainty of global climate change. However, functional genomic and biotechnological research in broomcorn millet has been hampered due to a lack of genetic tools such as transformation and genome‐editing techniques. Here, we successfully performed genome editing of broomcorn millet. We identified an elite variety, Hongmi, that produces embryogenic callus and has high shoot regeneration ability in in vitro culture. We established an Agrobacterium tumefaciens‐mediated genetic transformation protocol and a clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9‐mediated genome‐editing system for Hongmi. Using these techniques, we produced herbicide‐resistant transgenic plants and edited phytoene desaturase (PmPDS), which is involved in chlorophyll biosynthesis. To facilitate the rapid adoption of Hongmi as a model line for broomcorn millet research, we assembled a near‐complete genome sequence of Hongmi and comprehensively annotated its genome. Together, our results open the door to improving broomcorn millet using biotechnology.

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Plant genome engineering from lab to field—a Keystone Symposia report

Cited by 5 publications

References 112 publications

How Could the Use of Crop Wild Relatives in Breeding Increase the Adaptation of Crops to Marginal Environments?

How Could the Use of Crop Wild Relatives in Breeding Increase the Adaptation of Crops to Marginal Environments?

Harnessing the potential of CRISPR/Cas system for enhancing virus resistance in plants: Targets, strategies, and challenges

Establishment of genome‐editing system and assembly of a near‐complete genome in broomcorn millet

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