“…It is difficult, however, to construct the nuclease protein and a new TALEN protein must be generated for each DNA target site, which increases time and costs for development [29]. Additionally, the cut efficiency for TALENs varies depending on target sequence and TALENs cannot target methylated DNA [13]. As we know, DNA methylation of plant genome controls plant growth, development, transcription, DNA repair, gene expression, and cell differentiation, and is particularly involved in specific gene silencing.…”