Plant Gene Vectors and Genetic Transformation: Plant Viruses as Vectors

Gronenborn, Bruno; Matzeit, Volker

doi:10.1016/b978-0-12-715006-2.50011-0

Cited by 7 publications

(6 citation statements)

References 173 publications

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“…Using protoplast-derived cells of a Triticum monococcum suspension culture, Gronenborn and Matzeit (1989) were able to demonstrate the replication of a cloned WDV genome when it was applied as a genomic dimer. A cloned viral monomer did not lead to replicative molecules, unless the WDV genome had been released from the bacterial vector (pUC8) sequences.…”

Section: Resultsmentioning

confidence: 99%

Expression of engineered wheat dwarf virus in seed‐derived embryos

Töpfer

Gronenborn

Schaefer

et al. 1990

Physiologia Plantarum

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Delivery of DNA into dry and viable embryos of wheat by imbibition in DNA solution was detected by monitoring the transient expression of chimeric genes. The gene expression vectors used comprise of a neomycin phosphotransferase II (NPT II) reporter gene, under the control of various promoters or as part of a cloned plant virus genome. The genome of wheat dwarf virus (WDV), a monopartite Gemini virus of gramineae, has been used to amplify the NPT II gene in embryos of wheat, thereby leading to high levels of transient expression. Constructions were designed which permitted NPT II expression only if inter‐ or intramolecular recombination had occurred.

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Section: Resultsmentioning

confidence: 99%

Expression of engineered wheat dwarf virus in seed‐derived embryos

Töpfer

Gronenborn

Schaefer

et al. 1990

Physiologia Plantarum

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“…Moreover, recent findings concerning some small plant DNA viruses, the geminivirus [ 14], which do not code for their own DNA polymerase and thus use the host D N A replication machinery, strengthen the importance of studying the enzymes involved in plant cell D N A replication and repair with the aim of controlling the proliferation of plant D N A viruses, or other microbiological agents, that m a y use the host enzymes.…”

Section: Dna Polymerase C I (Pg)mentioning

confidence: 99%

A low molecular weight DNA polymerase from wheat embryos

1990

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The study of plant DNA polymerases lags far behind that concerning their animal or yeast counterpart. In this work we describe the first extensive purification to apparent homogeneity, as well as a detailed biochemical and immunological characterization, of a low molecular weight DNA polymerase (DNA polymerase CI) purified from wheat embryos. The monomeric enzyme is a basic protein having a molecular weight of 52 kDa. Polyclonal antibodies raised in rabbits against DNA polymerase CI did not inhibit animal DNA polymerases alpha and beta or wheat DNA polymerase A, whereas wheat DNA polymerases CII and B were much less affected than the CI enzyme. Several properties of enzyme CI were studied. Some known inhibitors of DNA polymerase activity including aphidicolin, phosphonoacetic acid and heparin, did not affect DNA polymerase CI while the activity of this enzyme was strongly inhibited by ddTTP and N-ethylmaleimide. The polyamine spermine decreased markedly the enzyme activity, while spermidine produced a strong stimulation at the same concentrations that spermine inhibited the enzyme. The best template for this enzyme is poly dA-oligo dT, although polymerase CI can recognize significantly some synthetic polyribonucleotide templates (poly rC-oligo dG, poly rA-oligo dT) but only at a given protein/template primer ratio. The enzyme is blocked at the amino terminus, thus preventing the automatic sequencing of the protein. The amino acid analysis showed a striking similarity with the animal low molecular weight DNA polymerase beta. The latter observation, as well as the effect of inhibitors (except N-ethylmaleimide which does not inhibit the animal polymerase) indicate that the DNA polymerase described in this work is a plant DNA polymerase very similar to the low molecular weight animal DNA polymerase beta, an enzyme believed to be involved in nuclear DNA repair.

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“…Plant DNA viruses, like geminiviruses and to a lesser extent caulimoviruses, have been used as extrachromosomal replicons for heterologous protein expression (reviewed in Gronenborn and Matzeit, 1989; Timmermans et al ., 1994). However, size limitation for encapsidation as well as complex gene expression are factors that have retarded progress in the field (Viaplana et al ., 2001).…”

Section: Introductionmentioning

confidence: 99%

In planta protein–protein interactions assessed using a nanovirus‐based replication and expression system

et al. 2002

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SummaryThe multipartite genome of the nanovirus Faba bean necrotic yellows virus, which consists of one gene on each DNA component, was exploited to construct a series of virus-based episomal vectors designed for transient replication and gene expression in plants. This nanovirus based expression system yields high levels of protein which allows isolation of recombinant protein and protein complexes from plant tissues. As examples, we demonstrated in planta interaction between the nanovirus F-box protein Clink and SKP1, a constituent of the ubiquitin-dependent protein turnover pathway. Thus, replicative nanovirus vectors provide a simple and ef®cient means for in planta characterization of protein-protein interaction.

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Plant Gene Vectors and Genetic Transformation: Plant Viruses as Vectors

Cited by 7 publications

References 173 publications

Expression of engineered wheat dwarf virus in seed‐derived embryos

Expression of engineered wheat dwarf virus in seed‐derived embryos

A low molecular weight DNA polymerase from wheat embryos

In planta protein–protein interactions assessed using a nanovirus‐based replication and expression system

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