Plant Flavonoids on Oxidative Stress-Mediated Kidney Inflammation

Alsawaf, Seba; Alnuaimi, Fatema; Afzal, Saba; Thomas, Rinku M.; Chelakkot, Ayshwarya Lakshmi; Ramadan, Wafaa; Hodeify, Rawad; Matar, Rachel; Merheb, Maxime; Siddiqui, Shoib Sarwar; Vazhappilly, Cijo George

doi:10.3390/biology11121717

Cited by 19 publications

(20 citation statements)

References 129 publications

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“…This is in line with how ISL is known to increase antioxidant effects. These results are in line with other research that has focused on how oxidative stress plays a part in the development of gentamicin-induced nephrotoxicity and how antioxidant therapy can help reduce its effects [18,19].…”

Section: Discussionsupporting

confidence: 91%

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In Vivo Antioxidant and Nephroprotective Effects of Ethanolic Extract of Carica papaya Seeds and Its Isolated Flavonoid on Gentamicin-Induced Nephrotoxicity in Wistar Albino Rats

Sandhya Rani,

Vedavijaya,

Podila

et al. 2024

Cureus

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BackgroundThe nephrotoxic side effects of gentamicin, a potent aminoglycoside antibiotic, significantly restrict its clinical use. Identifying compounds that can mitigate this nephrotoxicity is of paramount importance. The research examines how the ethanolic extract of Carica papaya seeds (EECPS) and isoliquiritigenin (ISL), a flavonoid separated from them, protect the kidneys and fight free radicals in gentamicin-treated Wistar albino rats. MethodologyA total of 48 mature Wistar albino rats were divided into eight groups, with each group consisting of six rats. The experimental setup included a normal control group receiving oral saline as a negative control, and a standard control group administered gentamicin intraperitoneally (IP) at 100 mg/kg body weight for 13 days to induce nephrotoxicity, followed by oral silymarin at 100 mg/kg body weight as a positive control from days 14 to 21. A toxicant control group was exposed to gentamicin IP without subsequent treatment. Two test groups were given 400 mg/kg and 800 mg/kg of EECPS orally after being given gentamicin. Three other test groups were given 20 mg/kg, 40 mg/kg, and 80 mg/kg of ISL orally after being given gentamicin. Serum levels of creatinine, urea, and blood urea nitrogen (BUN) were used to test renal function. Malondialdehyde (MDA), nitric oxide (NO), and reduced glutathione (GSH), which are signs of oxidative stress, were also measured in renal tissues. ResultsGentamicin administration markedly increased serum creatinine, urea, and BUN levels, confirming its nephrotoxic effect. Nephroprotection depended on the dose of EECPS and ISL used. It was found that 80 mg/kg of ISL had the most powerful effect, which was not what was thought at first. These treatments effectively reduced MDA and NO levels while enhancing GSH levels, exhibiting their strong antioxidant properties. Notably, the nephroprotective efficacy of these treatments exceeded that of silymarin, a known nephroprotective agent. Histopathological analysis confirmed reduced renal damage and enhanced tissue repair in the treated groups. ConclusionsThese findings demonstrate how effective EECPS and ISL are at shielding the kidneys from gentamicincaused damage. They do this by acting as antioxidants and nephroprotectants. Their ability to protect kidney function and fight oxidative stress makes them interesting as possible treatments for gentamicin-related kidney damage. These results advocate for further investigation into the utility of these natural compounds in the management of nephrotoxicity.

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Section: Discussionsupporting

confidence: 91%

“…We found that the antioxidant effects were also nephroprotective when we examined oxidative stress markers (MDA, NO, and GSH) in kidney tissue [18]. Following the treatment, there was a big drop in MDA and NO levels and a rise in GSH levels.…”

Section: Discussionmentioning

confidence: 88%

In Vivo Antioxidant and Nephroprotective Effects of Ethanolic Extract of Carica papaya Seeds and Its Isolated Flavonoid on Gentamicin-Induced Nephrotoxicity in Wistar Albino Rats

Sandhya Rani,

Vedavijaya,

Podila

et al. 2024

Cureus

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“…It is known that preeclamptic pregnancies are characterized by the presence of oxidative stress [26], an important process also reported in many other pathologies, including cancer [34,35]. Moreover, it has been reported that oxidative stress plays a key role in promoting the inflammation process [36,37]. Interestingly, we found that inducing oxidative stress in vitro significantly increased HTRA1 expression in both BeWo and HTR8/SVneo cell lines.…”

Section: Discussionsupporting

confidence: 61%

HTRA1 in Placental Cell Models: A Possible Role in Preeclampsia

Tossetta

Fantone

Giannubilo

et al. 2023

CIMB

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The HtrA serine peptidase 1 (HTRA1) is a multidomain secretory protein with serine–protease activity involved in the regulation of many cellular processes in both physiological and pathological conditions. HTRA1 is normally expressed in the human placenta, and its expression is higher in the first trimester compared to the third trimester, suggesting an important role of this serine protease in the early phases of human placenta development. The aim of this study was to evaluate the functional role of HTRA1 in in vitro models of human placenta in order to define the role of this serine protease in preeclampsia (PE). BeWo and HTR8/SVneo cells expressing HTRA1 were used as syncytiotrophoblast and cytotrophoblast models, respectively. Oxidative stress was induced by treating BeWo and HTR8/SVneo cells with H2O2 to mimic PE conditions in order to evaluate its effect on HTRA1 expression. In addition, HTRA1 overexpression and silencing experiments were performed to evaluate the effects on syncytialization, cell mobility, and invasion processes. Our main data showed that oxidative stress significantly increased HTRA1 expression in both BeWo and HTR8/SVneo cells. In addition, we demonstrated that HTRA1 has a pivotal role in cell motility and invasion processes. In particular, HTRA1 overexpression increased while HTRA1 silencing decreased cell motility and invasion in HTR8/SVneo cell model. In conclusion, our results suggest an important role of HTRA1 in regulating extravillous cytotrophoblast invasion and motility during the early stage of placentation in the first trimester of gestation, suggesting a key role of this serine protease in PE onset.

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“…Bax stands as a pro-apoptotic protein, on the contrary, Bcl2 exerts anti-apoptotic effects. So, the tissue Bax/Bcl2 ratio is commonly used to predict especially the severity of oxidative stress-related mitochondrial damage (18).…”

Section: Discussionmentioning

confidence: 99%

Protective Role of Ankaferd Blood Stopper on Cadmium-Induced Acute Nephrotoxicity

İlhan

Büyükbayram

2023

SDÜ Tıp Fakültesi Dergisi

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Objective Cadmium (Cd) is a very toxic and carcinogenic heavy metal that can cause harmful effects on human health. Toxicity may develop due to Cd exposure, especially in the kidneys. Ankaferd blood stopper (ABS) is a herbal mix that is used for its hemostatic properties in surgery. Also, ABS enhances wound and tissue healing. In this study, we aimed to evaluate the possible ameliorative effects of ABS in Cd-induced renal damage. Material and Methods Thirty-two male rats were randomly divided into 4 groups: control, Cd (cadmium chloride, 2.5 mg/kg single dose, i.p.), ABS (ABS, 1.5 ml/kg single dose i.p.), and Cd+ABS (cadmium chloride, 2.5 mg/kg single dose i.p.- ABS, 1.5 ml/kg single dose i.p.). At the end of the experiment, urea and creatinine levels were analyzed from the rats’ serum. In addition, total oxidant status (TOS), total antioxidant status (TAS) levels, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activity were measured spectrophotometrically from renal tissues. The oxidative stress index (OSI) was calculated from TOS and TAS levels. Also, we evaluated alterations in the mRNA expression of Bcl-2-associated X protein (Bax), B-cell-lymphoma-2 (Bcl-2), silenced information regulator 1 (SIRT1), and p53 in kidney tissue of rats by using the qRT-PCR method. Results In the Cd group, serum urea, creatinine levels, and tissue oxidative stress markers, TOS and OSI were higher while Gpx activity was significantly lower than in the control group. Also, the expression of p53 and in Bax/Bcl2 ratio increased in the Cd group. But, ABS treatment diminished urea, creatinine, TOS, OSI levels, and Bax/Bcl2 ratio, p53 expression in Cd applied group. Conclusion ABS showed protective effects by reducing oxidative stress and mitochondria-mediated apoptosis in acute Cd exposure.

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Plant Flavonoids on Oxidative Stress-Mediated Kidney Inflammation

Cited by 19 publications

References 129 publications

In Vivo Antioxidant and Nephroprotective Effects of Ethanolic Extract of Carica papaya Seeds and Its Isolated Flavonoid on Gentamicin-Induced Nephrotoxicity in Wistar Albino Rats

In Vivo Antioxidant and Nephroprotective Effects of Ethanolic Extract of Carica papaya Seeds and Its Isolated Flavonoid on Gentamicin-Induced Nephrotoxicity in Wistar Albino Rats

HTRA1 in Placental Cell Models: A Possible Role in Preeclampsia

Protective Role of Ankaferd Blood Stopper on Cadmium-Induced Acute Nephrotoxicity

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