Plant Flavonoid Production in Bacteria and Yeasts

Isogai, Shota; Tominaga, Masahiro; Kondo, Akihiko; Ishii, Jun

doi:10.3389/fceng.2022.880694

Cited by 3 publications

(1 citation statement)

References 123 publications

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“…Common metabolic strategies for enhancing the yields of flavonoids include the (a) removal of feedback inhibition points, (b) enhanced availability of precursors, (c) blocking of competitive pathways, (d) strain-dependent pathway balancing, and (e) co-factor recycling and biosynthesis [ 136 ]. These strategies are reviewed elsewhere in greater detail [ 137 , 138 ].…”

Section: Metabolic Engineering Of Flavonoid Pathwaysmentioning

confidence: 99%

Flavonoid Production: Current Trends in Plant Metabolic Engineering and De Novo Microbial Production

et al. 2023

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Flavonoids are secondary metabolites that represent a heterogeneous family of plant polyphenolic compounds. Recent research has determined that the health benefits of fruits and vegetables, as well as the therapeutic potential of medicinal plants, are based on the presence of various bioactive natural products, including a high proportion of flavonoids. With current trends in plant metabolite research, flavonoids have become the center of attention due to their significant bioactivity associated with anti-cancer, antioxidant, anti-inflammatory, and anti-microbial activities. However, the use of traditional approaches, widely associated with the production of flavonoids, including plant extraction and chemical synthesis, has not been able to establish a scalable route for large-scale production on an industrial level. The renovation of biosynthetic pathways in plants and industrially significant microbes using advanced genetic engineering tools offers substantial promise for the exploration and scalable production of flavonoids. Recently, the co-culture engineering approach has emerged to prevail over the constraints and limitations of the conventional monoculture approach by harnessing the power of two or more strains of engineered microbes to reconstruct the target biosynthetic pathway. In this review, current perspectives on the biosynthesis and metabolic engineering of flavonoids in plants have been summarized. Special emphasis is placed on the most recent developments in the microbial production of major classes of flavonoids. Finally, we describe the recent achievements in genetic engineering for the combinatorial biosynthesis of flavonoids by reconstructing synthesis pathways in microorganisms via a co-culture strategy to obtain high amounts of specific bioactive compounds

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Section: Metabolic Engineering Of Flavonoid Pathwaysmentioning

confidence: 99%

Flavonoid Production: Current Trends in Plant Metabolic Engineering and De Novo Microbial Production

et al. 2023

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Recent advancements in flavonoid production through engineering microbial systems

Hwang,

Noh,

Jung

2024

Biotechnol Bioproc E

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Effects of novel cellulase (Cel 906) and probiotic yeast fermentation on antioxidant and anti-inflammatory activities of vine tea (Ampelopsis grossedentata)

Hussain

et al. 2022

Front. Bioeng. Biotechnol.

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Vine tea (Ampelopsis grossedentata) is a plant resource with good nutritional and medicinal, and is widely consumed in China. This study aimed to develop a functional vine tea fermentation broth using microbial fermentation and cellulase degradation. First, the most suitable probiotics for vine tea fermentation were screened, and the fermentation conditions were optimized. Then, a new cellulase (Cel 906, MW076177) was added to evaluate the changes in the contents of effective substances and to study its efficacy. The results show that saccharomyces cerevisiae Y-401 was identified as the best strain, the optimal fermentation conditions were a time of 94.60 h, feeding concentration of 115.21 g/L, and temperature of about 34.97 °C. The vine tea fermentation broth has a strong inhibitory ability on 2,2′-azinobis3ethylbenzothiazoline-6-sulfonic acid (ABTS) (99.73%), peroxyl (53.15%), superoxide anion radicals (84.13%), and 1,1-Diphenyl-2trinitrophenylhydrazine (DPPH) (92.48%). It has a decent inhibitory impact on the cell viability, tyrosinase activity (32.25%), and melanin synthesis (63.52%) of B16-F10 melanoma cells induced by α-MSH. Inflammatory cell recruitment was reduced in a zebrafish inflammation model. Therefore, this vine tea fermented broth has strong antioxidant, anti-melanoma, and anti-inflammatory effects, and has healthcare potential as a probiotic tea.

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Plant Flavonoid Production in Bacteria and Yeasts

Cited by 3 publications

References 123 publications

Flavonoid Production: Current Trends in Plant Metabolic Engineering and De Novo Microbial Production

Flavonoid Production: Current Trends in Plant Metabolic Engineering and De Novo Microbial Production

Recent advancements in flavonoid production through engineering microbial systems

Effects of novel cellulase (Cel 906) and probiotic yeast fermentation on antioxidant and anti-inflammatory activities of vine tea (Ampelopsis grossedentata)

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