Plant-expressed HA as a seasonal influenza vaccine candidate

Shoji, Yasuhiro; Chichester, Jessica A.; Bi, Hong; Musiychuk, Konstantin; Rosa, Patricia de la; Goldschmidt, Lauren; Horsey, April; Ugulava, Natalia; Palmer, Gene A.; Mett, Vadim; Yusibov, Vidadi

doi:10.1016/j.vaccine.2008.03.045

Cited by 100 publications

(75 citation statements)

References 26 publications

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“…Several recent reports demonstrated that HA and HA1 fragment containing the majority of antigenic determinants are responsible for generation of virus-neutralizing antibodies [4,[11][12][13][14][15]. A transient expression system was successfully used to produce in 7 plants HA from A/Wyoming/03/03 (H3N2) virus that generated neutralizing antibodies in mice [11].…”

Section: Immunogenicity Of Ha1 Protein In Micementioning

confidence: 99%

“…A transient expression system was successfully used to produce in 7 plants HA from A/Wyoming/03/03 (H3N2) virus that generated neutralizing antibodies in mice [11]. However, in our plant-based system, despite the high serum titers of H5-specific question also remains to whether lack of neutralizing antibodies is due to the dosage, peptide folding, purity etc.…”

Section: Immunogenicity Of Ha1 Protein In Micementioning

confidence: 99%

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Immunological assessment of plant-derived avian flu H5/HA1 variants

Spitsin

Andrianov

Pogrebnyak

et al. 2009

Vaccine

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Section: Immunogenicity Of Ha1 Protein In Micementioning

confidence: 99%

Section: Immunogenicity Of Ha1 Protein In Micementioning

confidence: 99%

Immunological assessment of plant-derived avian flu H5/HA1 variants

Spitsin

Andrianov

Pogrebnyak

et al. 2009

Vaccine

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“…The most advanced transient expression system is based on the use of 'launch vectors' that combine components of plant viruses and binary plasmids, and are delivered by agroinfiltration 17,18 . Agroinfiltration of a launch vector based on Tobacco mosaic virus (TMV) has been successfully applied at lab scale to produce vaccine antigens against pathogens such as human papilloma virus 19 , Yersinia pestis 20 , influenza viruses A 21,22 , Bacillus anthracis 23 , and smallpox virus 24 in N. benthamiana leaves. Agrobacterium-mediated transient expression is also a promising method for the simultaneous production of multiple proteins 2, [25][26][27] .…”

Section: Introductionmentioning

confidence: 99%

Optimization and Utilization of <em>Agrobacterium</em>-mediated Transient Protein Production in <em>Nicotiana</em>

Shamloul

Trusa

Mett

et al. 2014

JoVE

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Agrobacterium-mediated transient protein production in plants is a promising approach to produce vaccine antigens and therapeutic proteins within a short period of time. However, this technology is only just beginning to be applied to large-scale production as many technological obstacles to scale up are now being overcome. Here, we demonstrate a simple and reproducible method for industrial-scale transient protein production based on vacuum infiltration of Nicotiana plants with Agrobacteria carrying launch vectors. Optimization of Agrobacterium cultivation in AB medium allows direct dilution of the bacterial culture in Milli-Q water, simplifying the infiltration process. Among three tested species of Nicotiana, N. excelsiana (N. benthamiana × N. excelsior) was selected as the most promising host due to the ease of infiltration, high level of reporter protein production, and about two-fold higher biomass production under controlled environmental conditions. Induction of Agrobacterium harboring pBID4-GFP (Tobacco mosaic virus-based) using chemicals such as acetosyringone and monosaccharide had no effect on the protein production level. Infiltrating plant under 50 to 100 mbar for 30 or 60 sec resulted in about 95% infiltration of plant leaf tissues. Infiltration with Agrobacterium laboratory strain GV3101 showed the highest protein production compared to Agrobacteria laboratory strains LBA4404 and C58C1 and wild-type Agrobacteria strains at6, at10, at77 and A4. Co-expression of a viral RNA silencing suppressor, p23 or p19, in N. benthamiana resulted in earlier accumulation and increased production (15-25%) of target protein (influenza virus hemagglutinin).

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“…20 There are many examples of recombinant proteins manufactured in plants which are being used as effective vaccines for diseases such as influenza, anthrax, and plague. 21,22,24,28 However, heterologous protein expression in plants does face many hurdles that must be overcome. Recombinant proteins which express at low levels in plants require considerable effort to isolate from the milieu of total plant proteins.…”

Section: Introductionmentioning

confidence: 99%