Plant endosomes as protein sorting hubs

González-Solís, Ariadna; Berryman, Elizabeth; Otegui, Marisa S.

doi:10.1002/1873-3468.14425

Cited by 15 publications

(10 citation statements)

References 180 publications

(231 reference statements)

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“…Retromer recycles vacuolar sorting receptors from the TGN (Niemes et al, 2010). It also mediates the retrograde recycling of biosynthetic cargo receptors back to the TGN/Golgi and endocytic cargos back to the PM (González Solís et al, 2022). Retromer subunits VPS29 (1.5 fold upregulated in the rhd2-1 mutant) and VPS35A are important for prevacuolar compartment (PVC) function in Arabidopsis (Nodzyński et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Remodelling of cytoskeleton and plasma membrane proteins contributes to drought sensitivity of Arabidopsisrhd2mutant

Takáč

Kuběnová

Šamajová

et al. 2023

Preprint

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NADPH oxidases are enzymes localised in the plasma membrane and emitting superoxide to the extracellular space. By production of superoxide as one type of reactive oxygen species (ROS), they exert pleiotropic functions in plant development and various stress responses. Arabidopsis thaliana RESPIRATORY BURST OXIDASE HOMOLOG PROTEIN C/ROOT HAIR DEFECTIVE 2 (AtRBOHC/RHD2) is an NADPH oxidase with preferential gene expression in roots. Polar localisation and ROS production by this enzyme are essential for root hair elongation. However, the proteome-wide and physiological consequences of RBOHC/RHD2 mutations are unknown. To find out potential new functions of AtRBOHC/RHD2, we employed a differential proteomic analysis of Arabidopsis rhd2-1 mutant, carrying a loss-of-function mutation in RBOHC/RHD2. Proteomic analyses that were validated with independent biochemical, phenotypical and advanced microscopy methods, showed quantitative deregulation of proteins involved in abiotic and biotic stress response, metabolism, vesicular transport and cell wall modification. Considerable differences in the differential proteomes between roots and above-ground parts were found in the mutant. The altered abundance of aquaporins and homeostasis of transmembrane pumps and transporters most likely determine the higher sensitivity of Arabidopsis rhd2-1 mutant to drought.

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Section: Discussionmentioning

confidence: 99%

Remodelling of cytoskeleton and plasma membrane proteins contributes to drought sensitivity of Arabidopsisrhd2mutant

Takáč

Kuběnová

Šamajová

et al. 2023

Preprint

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“…Less obvious developmental phenotypes are observed in Arabidopsis atg14a atg14b double mutants, although they are defective in autophagy ( Liu et al., 2020 ). Generally, plants with compromised functions of vacuoles and endosomes display lethality or severe developmental abnormality ( González Solís et al., 2022 ), whereas autophagy-deficient plants do not. Instead, the autophagy mutants are hypersensitive to different types of abiotic and biotic stress ( Qi et al., 2021 ).…”

Section: Introductionmentioning

confidence: 99%

The phosphatidylinositol 3-phosphate effector FYVE3 regulates FYVE2-dependent autophagy in Arabidopsis thaliana

Kim

Jung²,

Song³

et al. 2023

Front. Plant Sci.

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Phosphatidylinositol 3-phosphate (PI3P) is a signaling phospholipid that play a key role in endomembrane trafficking, specifically autophagy and endosomal trafficking. However, the mechanisms underlying the contribution of PI3P downstream effectors to plant autophagy remain unknown. Known PI3P effectors for autophagy in Arabidopsis thaliana include ATG18A (Autophagy-related 18A) and FYVE2 (Fab1p, YOTB, Vac1p, and EEA1 2), which are implicated in autophagosome biogenesis. Here, we report that FYVE3, a paralog of plant-specific FYVE2, plays a role in FYVE2-dependent autophagy. Using yeast two-hybrid and bimolecular fluorescence complementation assays, we determined that the FYVE3 protein was associated with autophagic machinery containing ATG18A and FYVE2, by interacting with ATG8 isoforms. The FYVE3 protein was transported to the vacuole, and the vacuolar delivery of FYVE3 relies on PI3P biosynthesis and the canonical autophagic machinery. Whereas the fyve3 mutation alone barely affects autophagic flux, it suppresses defective autophagy in fyve2 mutants. Based on the molecular genetics and cell biological data, we propose that FYVE3 specifically regulates FYVE2-dependent autophagy.

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“…Plants regulate nutrient uptake and translocation through a variety of transport proteins. The abundance of these nutrient transporters is regulated through multiple steps, including RNA transcription, protein translation, and post-Golgi trafficking processes (i.e., secretion, endocytosis, recycling, and vacuolar transport) (Paez Valencia et al, 2016; Gu et al, 2017; Ivanov and Vert, 2021; González Solís et al, 2022). In plants, specific mineral transporters located in the plasma membrane (PM), such as IRON-REGULATED TRANSPORTER 1 (IRT1), PHOSPHATE TRANSPORTER 1 (PHT1), and REQUIRES HIGH BORON 1 (BOR1), undergo ubiquitination and subsequent vacuolar sorting for degradation in response to an increase in their substrate concentration (Takano et al, 2005; Kerkeb et al, 2008; Bayle et al, 2011; Barberon et al, 2011; Kasai et al, 2011; Huang et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Rapid Vacuolar Sorting of the Borate Transporter BOR1 Requires the Adaptor Protein Complex AP-4 in Arabidopsis

Yoshinari,

Shimizu,

Hosokawa

et al. 2023

Preprint

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Plants maintain nutrient homeostasis by controlling the activities and abundance of nutrient transporters. InArabidopsis thaliana, the borate (B) transporter BOR1 plays a role in the efficient translocation of B under low-B conditions. BOR1 undergoes polyubiquitination in the presence of sufficient B and is then transported to the vacuole via multivesicular bodies (MVBs) to prevent B accumulation in tissues at a toxic level. A previous study indicated that BOR1 physically interacts with μ subunits of adaptor protein complexes AP-3 and AP-4, both involved in vacuolar sorting pathways. In this study, we investigated the roles of AP-3 and AP-4 subunits in BOR1 trafficking in Arabidopsis. The lack of AP-3 subunits did not affect either vacuolar sorting or polar localization of BOR1-GFP, whereas the absence of AP-4 subunits resulted in a delay in high-B-induced vacuolar sorting without affecting polar localization. Super-resolution microscopy revealed a rapid sorting of BOR1-GFP into AP-4-positive spots in thetrans-Golgi network (TGN) upon high-B supply. These results indicate that AP-4 is involved in sequestration of ubiquitinated BOR1 into a TGN-specific subdomain “vacuolar-trafficking zone,” and is required for efficient sorting to MVB and vacuole. Our findings elucidate the rapid vacuolar sorting process facilitated by AP-4 in plant nutrient transporters.Subject areas(7) Membrane and transport

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Plant endosomes as protein sorting hubs

Cited by 15 publications

References 180 publications

Remodelling of cytoskeleton and plasma membrane proteins contributes to drought sensitivity of Arabidopsisrhd2mutant

Remodelling of cytoskeleton and plasma membrane proteins contributes to drought sensitivity of Arabidopsisrhd2mutant

The phosphatidylinositol 3-phosphate effector FYVE3 regulates FYVE2-dependent autophagy in Arabidopsis thaliana

Rapid Vacuolar Sorting of the Borate Transporter BOR1 Requires the Adaptor Protein Complex AP-4 in Arabidopsis

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