Plant-endophytes interaction influences the secondary metabolism in Echinacea purpurea (L.) Moench: an in vitro model

Maggini, Valentina; Leo, Marinella De; Mengoni, Alessio; Gallo, Eugenia; Miceli, Elisangela; Reidel, Rose Vanessa Bandeira; Biffi, Sauro; Pistelli, Luisa; Fani, Renato; Firenzuoli, Fabio; Bogani, Patrizia

doi:10.1038/s41598-017-17110-w

Cited by 73 publications

(60 citation statements)

References 36 publications

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“…Evidence from our lab 1–4 and others 5,6 supports the theory that the efficacy of E. purpurea against respiratory infections is dependent, at least in part, on its bacterial community (microbiome). Bacterial components of this microbiome can directly impact immune function 1–5 and plant-endophyte interactions can alter secondary metabolite production of the anti-inflammatory alkylamides 6 . The immune-activating bacterial components may exhibit therapeutic effects against respiratory infection comparable to those reported in clinical research on probiotic bacteria 7 .…”

Section: Introductionsupporting

confidence: 69%

Plant microbiome-dependent immune enhancing action of Echinacea purpurea is enhanced by soil organic matter content

Haron

Tyler

Chandra

et al. 2019

Sci Rep

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We previously demonstrated that extracts from Echinacea purpurea material varied substantially in their ability to activate macrophages in vitro and that this variation was due to differences in their content of bacterial components. The purpose of the current study was to identify soil conditions (organic matter, nitrogen, and moisture content) that alter the macrophage activation potential of E. purpurea and determine whether these changes in activity correspond to shifts in the plant-associated microbiome. Increased levels of soil organic matter significantly enhanced macrophage activation exhibited by the root extracts of E. purpurea (p < 0.0001). A change in soil organic matter content from 5.6% to 67.4% led to a 4.2-fold increase in the macrophage activation potential of extracts from E. purpurea. Bacterial communities also differed significantly between root materials cultivated in soils with different levels of organic matter (p < 0.001). These results indicate that the level of soil organic matter is an agricultural factor that can alter the bacterial microbiome, and thereby the activity, of E. purpurea roots. Since ingestion of bacterial preparation (e.g., probiotics) is reported to impact human health, it is likely that the medicinal value of Echinacea is influenced by cultivation conditions that alter its associated bacterial community.

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Section: Introductionsupporting

confidence: 69%

Plant microbiome-dependent immune enhancing action of Echinacea purpurea is enhanced by soil organic matter content

Haron

Tyler

Chandra

et al. 2019

Sci Rep

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“…In a study involving 260 isolates of endophytic actinobacteria, majority of them were seen to be inhibiting plant pathogens, and thus had a protective role for protection of the hosts against diseases (Palaniyandi et al, 2013 ). The type of interaction among the actinobacteria and their host-plants seems to be important in suppression of diseases (Hasegawa et al, 2006 ; Maggini et al, 2017 ). Leaf blight disease of rice was cured to a greater extent by S. platensis (Wan et al, 2008 ).…”

Section: Opportunities For Application Of Endophytic Actinobacteria Imentioning

confidence: 99%

Diversity and Applications of Endophytic Actinobacteria of Plants in Special and Other Ecological Niches

2018

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Actinobacteria are wide spread in nature and represent the largest taxonomic group within the domain Bacteria. They are abundant in soil and have been extensively explored for their therapeutic applications. This versatile group of bacteria has adapted to diverse ecological habitats, which has drawn considerable attention of the scientific community in recent times as it has opened up new possibilities for novel metabolites that may help in solving some of the most challenging problems of the day, for example, novel drugs for drug-resistant human pathogens, affordable means to maintain ecological balance in various habitats, and alternative practices for sustainable agriculture. Traditionally, free dwelling soil actinobacteria have been the subject of intensive research. Of late, symbiotic actinobacteria residing as endophytes within the plant tissues have generated immense interest as potential source of novel compounds, which may find applications in medicine, agriculture, and environment. In the light of these possibilities, this review focuses on the diversity of endophytic actinobacteria isolated from the plants of extreme habitats and specific ecological niches. Furthermore, an attempt has been made to assign chemical class to the compounds obtained from endophytic actinobacteria. Potential therapeutic applications of these compounds and the utility of endophytic actinobacteria in agriculture and environment are discussed.

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“…Moreover, plant-associated endophytic bacteria colonize plants without apparently eliciting defense responses or injuring the plant. In some cases, endophytes induce a valuable promotion of plant growth [6–9], confer resistance to environmental stresses [10, 11] or contribute to ameliorate plant physical-chemical properties [12–16].…”

Section: Introductionmentioning

confidence: 99%

Tissue specificity and differential effects on in vitro plant growth of single bacterial endophytes isolated from the roots, leaves and rhizospheric soil of Echinacea purpurea

et al. 2019

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Background Echinacea -endophyte interaction might affect plant secondary metabolites content and influence bacterial colonization specificity and plant growth, but the underlying mechanisms need deepening. An in vitro model, in which E. purpurea axenic plants as host species and E. angustifolia and Nicotiana tabacum as non-host species inoculated with single endophytes isolated from stem/leaf, root and rhizospheric soil, were used to investigate bacterial colonization. Results Colonization analysis showed that bacteria tended to reach tissues from which they were originally isolated (tissue-specificity) in host plants but not in non-host ones (species-specificity). Primary root elongation inhibition as well as the promotion of the growth of E. purpurea and E. angustifolia plants were observed and related to endophyte-produced indole-3-Acetic Acid. Bacteria-secreted substances affected plant physiology probably interacting with plant regulators. Plant metabolites played an important role in controlling the endophyte growth. Conclusions The proposed in vitro infection model could be, generally used to identify novel bioactive compounds and/or to select specific endophytes contributing to the host metabolism properties. Electronic supplementary material The online version of this article (10.1186/s12870-019-1890-z) contains supplementary material, which is available to authorized users.

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Plant-endophytes interaction influences the secondary metabolism in Echinacea purpurea (L.) Moench: an in vitro model

Cited by 73 publications

References 36 publications

Plant microbiome-dependent immune enhancing action of Echinacea purpurea is enhanced by soil organic matter content

Plant microbiome-dependent immune enhancing action of Echinacea purpurea is enhanced by soil organic matter content

Diversity and Applications of Endophytic Actinobacteria of Plants in Special and Other Ecological Niches

Tissue specificity and differential effects on in vitro plant growth of single bacterial endophytes isolated from the roots, leaves and rhizospheric soil of Echinacea purpurea

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