Plant-derived nanovesicles: Further exploration of biomedical function and application potential

Li, Aixue; Li, Dan; Gu, Yongwei; Liu, Rongmei; Tang, Xin‐Ying; Zhao, Yunan; Qi, Fu; Wei, Ji‐Fu; Liu, Jiyong

doi:10.1016/j.apsb.2022.12.022

Cited by 19 publications

(9 citation statements)

References 170 publications

(303 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The zeta potential was also measured to assess the stability of PDENs in suspension and their adsorption capacity on the target cell membrane [ 27 ]. PDENs in general have negative zeta potential that lies between -100 and 0 mV, which varies between different plant sources [ 28 ]. The total protein concentration of PDENs were measured using bicinchoninic acid assay (BCA Assay), which based on the reaction between Cu + ion and sodium salt from bicinchoninic acid in an alkaline solution, producing a purple-colored solution that absorbs light at 562 nm [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation of plant-derived exosome-like nanoparticles (PDENs) from Solanum nigrum L. berries and Their Effect on interleukin-6 expression as a potential anti-inflammatory agent

Emmanuela,

Muhammad,

Iriawati

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Inflammation is a temporary response of the immune system that can be treated using common anti-inflammatory drugs. However, prolonged use of these drugs increases the risk of adverse side effects. Accordingly, there is an increasing need for alternative treatments for inflammation with fewer side effects. Exosomes are extracellular vesicles secreted by most eukaryotic cells and have been studied as a candidate for cell-free therapy for inflammatory diseases due to their immunomodulatory and anti-inflammatory properties. In recent years, the focus of exosome research has shifted from animal cell-derived exosomes to plant-derived exosome-like nanoparticles (PDENs). Plant-derived exosome-like nanoparticles (PDENs) are easier to obtain, have minimal safety concerns, and can be produced in higher quantities and lower cost than exosomes derived from animal cells. In this study, the isolation and analysis of the anti-inflammatory potential of PDENs from black nightshade berries (Solanum nigrum L.) were carried out. The results of isolation and characterization showed that PDENs had a spherical morphology, measuring around 107 nm with zeta potential of -0.6 mV, and had a protein concentration of 275.38 μg/mL. PDENs were also shown to be internalized by RAW264.7 macrophage cell line after 2 hours of incubation and had no cytotoxicity effect up to the concentration of 2.5 μg/mL. Furthermore, exposure to several doses of PDENs to the LPS-stimulated RAW264.7 cell significantly decreased the expression of pro-inflammatory cytokine gene IL-6, as well as the expression of IL-6 protein up to 97,28%. GC-MS analysis showed the presence of neral, a monoterpene compound with known anti-inflammatory properties, which may contribute to the anti-inflammatory activity of PDENs isolated from Solanum nigrum L. berries. Taken together, the present study was the first to isolate and characterize PDENs from Solanum nigrum L. berries. The results of this study also demonstrated the anti-inflammatory activity of PDEN by suppressing the production of IL-6 in LPS-stimulated RAW264.7 cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation of plant-derived exosome-like nanoparticles (PDENs) from Solanum nigrum L. berries and Their Effect on interleukin-6 expression as a potential anti-inflammatory agent

Emmanuela,

Muhammad,

Iriawati

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…At this point, a centrifugal force of between 100,000 and 150,000 g is selected for the second centrifugation. ( Li et al, 2023a ). (See Figure 5 ).…”

Section: Different Methods Of Isolating Extracellular Vesiclesmentioning

confidence: 99%

Plant and mammalian-derived extracellular vesicles: a new therapeutic approach for the future

Sall,

Flaviu

2023

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Background: In recent years, extracellular vesicles have been recognized as important mediators of intercellular communication through the transfer of active biomolecules (proteins, lipids, and nucleic acids) across the plant and animal kingdoms and have considerable roles in several physiological and pathological mechanisms, showing great promise as new therapeutic strategies for a variety of pathologies.Methods: In this study, we carefully reviewed the numerous articles published over the last few decades on the general knowledge of extracellular vesicles, their application in the therapy of various pathologies, and their prospects as an approach for the future.Results: The recent discovery and characterization of extracellular vesicles (EVs) of diverse origins and biogenesis have altered the current paradigm of intercellular communication, opening up new diagnostic and therapeutic perspectives. Research into these EVs released by plant and mammalian cells has revealed their involvement in a number of physiological and pathological mechanisms, such as embryonic development, immune response, tissue regeneration, and cancer. They are also being studied as potential biomarkers for disease diagnosis and vectors for drug delivery.Conclusion: Nanovesicles represent powerful tools for intercellular communication and the transfer of bioactive molecules. Their molecular composition and functions can vary according to their origin (plant and mammalian), so their formation, composition, and biological roles open the way to therapeutic applications in a variety of pathologies, which is arousing growing interest in the scientific community.Clinical Trial Registration:ClinicalTrials.gov identifier: NCT03608631

show abstract

“…Hence, PDNVs are sustainable, cost‐effective, and renewable nanoplatforms generated from natural sources. The unique properties of PDNVs, coupled with the vast variety and diversity of plants, open up a multitude of possibilities for innovation and advancement in nanotechnology (Pinedo et al 2021, Chen et al 2023b, Li et al 2023a). A research study provides substantial evidence that supports the use of Catharanthus roseus (L.) Don leaves‐derived nanovesicles (CLDNVs) as an exceptional nano‐biomaterial (Ou et al 2023).…”

Section: Introductionmentioning

confidence: 99%

Plant‐derived nanovesicles offer a promising avenue for anti‐aging interventions

Meng,

Sun,

et al. 2024

Physiologia Plantarum

View full text Add to dashboard Cite

Over the past few years, the study of plant‐derived nanovesicles (PDNVs) has emerged as a hot topic of discussion and research in the scientific community. This remarkable interest stems from their potential role in facilitating intercellular communication and their unique ability to deliver biologically active components, including proteins, lipids, and miRNAs, to recipient cells. This fascinating ability to act as a molecular courier has opened up an entirely new dimension in our understanding of plant biology. The field of research focusing on the potential applications of PDNVs is still in its nascent stages. However, it has already started gaining traction due to the growing interest in its possible use in various branches of biotechnology and medicine. Their unique properties and versatile applications offer promising future research and development prospects in these fields. Despite the significant progress in our understanding, many unanswered questions and mysteries surround the mechanisms by which PDNVs function and their potential applications. There is a dire need for further extensive research to elucidate these mechanisms and explore the full potential of these fascinating vesicles. As the technology at our disposal advances and our understanding of PDNVs deepens, it is beyond doubt that PDNVs will continue to be a subject of intense research in anti‐aging therapeutics. This comprehensive review is designed to delve into the fascinating and multifaceted world of PDNV‐based research, particularly focusing on how these nanovesicles can be applied to anti‐aging therapeutics.

show abstract

Plant-derived nanovesicles: Further exploration of biomedical function and application potential

Cited by 19 publications

References 170 publications

Isolation of plant-derived exosome-like nanoparticles (PDENs) from Solanum nigrum L. berries and Their Effect on interleukin-6 expression as a potential anti-inflammatory agent

Isolation of plant-derived exosome-like nanoparticles (PDENs) from Solanum nigrum L. berries and Their Effect on interleukin-6 expression as a potential anti-inflammatory agent

Plant and mammalian-derived extracellular vesicles: a new therapeutic approach for the future

Plant‐derived nanovesicles offer a promising avenue for anti‐aging interventions

Contact Info

Product

Resources

About