Plant-derived compounds regulate formation of the insect juvenile hormone receptor complex

Lee, Seok-Hee; Ha, Kyu Baik; Park, Dong Hwan; Fāng, Yīng; Kim, Jong Hoon; Park, Min Gu; Woo, Ra Mi; Kim, Woo Jin; Park, Il-Kwon; Choi, Jae Young; Je, Yeon Ho

doi:10.1016/j.pestbp.2018.06.013

Cited by 24 publications

(13 citation statements)

References 23 publications

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“…Furthermore, RGA can confirm the antagonist activity of chemicals of interest. 58,78,79,90,122,123,134,135) In comparison with agonist screening, however, the general cytotoxicity of test chemicals that may repress reporter gene expression in a receptor-independent manner should be carefully considered in antagonist assays. 80) RGA using a specifically engineered reporter gene construct to positively select for a decrease in receptor activity may allow for the effective screening of antagonists from a large pool of chemical compounds.…”

Section: Resultsmentioning

confidence: 99%

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Reporter gene assays for screening and identification of novel molting hormone- and juvenile hormone-like chemicals

Ito‐Harashima

Yagi

2021

J. Pestic. Sci.

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A reporter gene assay (RGA) is used to investigate the activity of synthetic chemicals mimicking the molting hormones (MHs) and juvenile hormones (JHs) of insects, so-called insect growth regulators (IGRs). The MH receptor, a heterodimer of the ecdysone receptor (EcR) and ultraspiracle (USP), and the JH receptor Methoprene-tolerant (Met) are ligand-dependent transcription factors. Ligand-bound EcR-USP and Met bind to specific cis-acting DNA elements, referred to as the ecdysone-responsive element (EcRE) and the JH-responsive element (JHRE), respectively, in order to transactivate target genes. Insect hormone-induced transactivation systems have been reconstituted by the introduction of reporter genes under the control of EcRE and JHRE, or two-hybrid reporter genes, into insect, mammalian, and yeast cells expressing receptor proteins. RGA is easy to use and convenient for examining the MHand JH-like activities of synthetic chemicals and is suitable for the high-throughput screening of novel structural classes of chemicals targeting EcR-USP and Met.

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Section: Resultsmentioning

confidence: 99%

“…These RGAs were used to screen for plant-derived compounds with agonist or antagonist activities. 122,123)…”

Section: Rga Systems For Detecting Jhs and Jhasmentioning

confidence: 99%

Reporter gene assays for screening and identification of novel molting hormone- and juvenile hormone-like chemicals

Ito‐Harashima

Yagi

2021

J. Pestic. Sci.

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“…Synthetic insecticides share some of these effects with botanicals, such as the neurotoxic effects by inhibiting acetylcholinesterase, blocking receptors for GABA or octopamine, and stopping development due to insect growth regulator analogs. Still, some insecticidal modes of action in botanicals, as observed by Kelly et al [ 48 ], are novel or rarely explored in control strategies, such as inhibition of mitochondrial respiration or detoxification enzymes, phototoxicity, generation of ROS, and midgut damage [ 26 , 49 , 50 ]. The use of these substances or the combination with other methods presents a potential alternative to reduce the impact of insecticide resistance [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

High larvicidal efficacy of yeast-encapsulated orange oil against Aedes aegypti strains from Brazil

et al. 2021

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Background Botanical substances such as essential oils (EOs) have demonstrated insecticidal properties and are a valid option for vector control. However, free EOs are unreliable as mosquito larvicides due their easy degradation by environmental exposure to ultraviolet light and higher temperatures. Here, we assessed the efficacy of a mosquito larvicide based on orange oil in a yeast-based delivery system against Aedes aegypti strains with different resistance status towards chemical neurotoxic insecticides. This larvicide preparation was physicochemically characterized in a previous report. Methods Larvae of four Ae. aegypti strains from different regions of Brazil and different resistance profiles for deltamethrin (pyrethroid) and temephos (organophosphate) were tested against yeast-encapsulated orange oil (YEOO) in laboratory conditions for measurement of LC50 and LC90 values. The same assays were performed with the Belo Horizonte strain under environmental conditions (natural light and temperature). The resistance profiles of these strains were compared to the Rockefeller reference strain in all conditions. Results YEOO was found to be a highly active larvicide (LC50 < 50 mg/L) against all Ae. aegypti strains tested in both laboratory conditions (LC50 = 8.1–24.7 mg/L) and environmental conditions with natural light and temperature fluctuation (LC50 = 20.0–49.9 mg/L). Moreover, all strains were considered susceptible (RR < 5) to YEOO, considering resistance ratios calculated based on the Rockefeller strain. The resistance ratios were only higher than 2.5 for LC90–95 of Belo Horizonte in the laboratory, probably due the higher heterogeneity associated with older egg papers (> 5 months). Conclusion YEOO demonstrates high larvicidal activity against Ae. aegypti strains with resistant phenotypes for deltamethrin (PY) and temephos (OP). This larvicidal activity suggests the potential for the development of YEOO as an alternative intervention to synthetic insecticides in integrated vector management programs, for populations with resistance to commonly used insecticides. Graphic Abstract

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“…Plant-based therapies for many diseases have been studied for a long time. Research is currently being conducted to assess the application of plant-derived components such as extracts and essential oils for medical treatments [1,2] and insecticides [3]. Most importantly, aromatherapy and massage are used in clinical settings [4,5] and in sports medicine [6] as complementary and alternative therapies.…”

Section: Introductionmentioning

confidence: 99%

Acute Effects of Transdermal Administration of Jojoba Oil on Lipid Metabolism in Mice

Matsumoto

Tominaga

et al. 2019

Medicina

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Background and objectives: Aroma therapy is a complementary therapy using essential oils diluted with carrier oils. Jojoba oils have been widely used as carrier oils. However, limited information is available regarding their effects on blood biochemical parameters. This study aimed to investigate the effect of transdermal administration of jojoba oil on blood biochemical parameters in mice. Materials and Methods: Eight-week-old male hairless mice were randomly divided into naïve control and treatment groups. In the treatment group, mice were topically administered 4 μL of jojoba oil, per gram of body weight, on the dorsa 30 min before euthanasia. Thereafter, serum biochemical parameters were assayed, and gene expression was analyzed in various tissues via a real-time polymerase chain reaction. Results: Serum non-esterified fatty acid (NEFA) levels increased significantly 30 min after topical application of jojoba oil (p < 0.05). Atgl was significantly upregulated in the liver (p < 0.05), and Atgl upregulation in the liver was positively correlated with serum NEFA levels (r = 0.592, p < 0.05). Furthermore, a trend of decreasing fatty acid trafficking-related gene (FABPpm, FATP-1, FATP-3, and FATP-4) expression in the skin after topical application of jojoba oil (p = 0.067, 0.074, 0.076, and 0.082, respectively) was observed. Conclusions: Serum NEFA levels were elevated 30 min after transdermal administration of jojoba oil. The mechanisms of elevated serum NEFA levels might be related to both enhanced lipolysis in the liver and reduced fatty acid trafficking in the skin.

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Plant-derived compounds regulate formation of the insect juvenile hormone receptor complex

Cited by 24 publications

References 23 publications

Reporter gene assays for screening and identification of novel molting hormone- and juvenile hormone-like chemicals

Reporter gene assays for screening and identification of novel molting hormone- and juvenile hormone-like chemicals

High larvicidal efficacy of yeast-encapsulated orange oil against Aedes aegypti strains from Brazil

Acute Effects of Transdermal Administration of Jojoba Oil on Lipid Metabolism in Mice

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