Plant-derived antimicrobials reduce Listeria monocytogenes virulence factors in vitro, and down-regulate expression of virulence genes

Upadhyay, Abhinav; Johny, Anup Kollanoor; Amalaradjou, Mary Anne Roshni; Baskaran, Sangeetha Ananda; Kim, Kwang Sik; Venkitanarayanan, Kumar

doi:10.1016/j.ijfoodmicro.2012.04.018

Cited by 89 publications

(68 citation statements)

References 51 publications

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“…We previously observed that SICs of trans-cinnamaldehyde (TC), carvacrol (CR) and thymol (TY) inhibited virulence properties of Listeria monocytogenes (Upadhyay et al, 2012). Additionally, the SICs of TC were found to suppress attaching and invading abilities of uropathogenic Escherichia coli in human urinary tract epithelial cells, by down-regulating the genes critical for host tissue colonization (Amalaradjou et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Eugenol in combination with lactic acid bacteria attenuates Listeria monocytogenes virulence in vitro and in invertebrate model Galleria mellonella

Upadhyay

Upadhyaya

Mooyottu

et al. 2016

Journal of Medical Microbiology

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Listeria monocytogenes is a human enteric pathogen that causes severe foodborne illness in high-risk populations. Crossing the intestinal barrier is the first critical step for Listeria monocytogenes infection. Therefore, reducing L. monocytogenes colonization and invasion of intestinal epithelium and production of virulence factors could potentially control listeriosis in humans. This study investigated the efficacy of sub-inhibitory concentration (SIC) of the plantderived antimicrobial eugenol, either alone, or in combination with five lactic acid bacteria (LAB), namely Bifidobacterium bifidum (NRRL-B41410), Lactobacillus reuteri (B-14172), Lactobacillus fermentum (B-1840), Lactobacillus plantarum (B-4496) and Lactococcus lactis subspecies lactis (B-633) in reducing Listeria monocytogenes adhesion to and invasion of human intestinal epithelial cells (Caco-2). Additionally, the effect of the aforementioned treatments on Listeria monocytogenes listeriolysin production, epithelial E-cadherin binding and expression of virulence genes was investigated. Moreover, the in vivo efficacy of eugenol-LAB treatments in reducing Listeria monocytogenes virulence in the invertebrate model Galleria mellonella was studied. Eugenol and LAB, either alone or in combination, significantly reduced Listeria monocytogenes adhesion to and invasion of intestinal cells (P < 0.05). Moreover, eugenol-LAB treatments decreased Listeria monocytogenes haemolysin production, E-cadherin binding and virulence gene expression (P < 0.05). In addition, the eugenol-LAB treatments significantly enhanced the survival rates of G. mellonella infected with lethal doses of Listeria monocytogenes (P < 0.05). The results highlight the antilisterial effect of eugenol either alone or in combination with LAB, and justify further investigations in a mammalian model.

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Section: Discussionmentioning

confidence: 99%

Eugenol in combination with lactic acid bacteria attenuates Listeria monocytogenes virulence in vitro and in invertebrate model Galleria mellonella

Upadhyay

Upadhyaya

Mooyottu

et al. 2016

Journal of Medical Microbiology

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“…18 The SIC of chitosan was determined using previously published protocol. 21 Briefly, 24 well polystyrene plates (Costar, Corning, NY, USA) containing CEB (2 mL/well) supplemented with two-fold dilutions of chitosan (0, 0.2, 0.1, 0.05, 0.025, 0.0125 and 0.00625%), were inoculated with ~5.0 log CFU of C. jejuni wild strain, followed by incubation at 42 ºC for 24 h. Bacterial growth was determined by culturing on CLA agar plates. The highest concentration of chitosan that did not inhibit C. jejuni growth during mid-log (8 h), and stationary phase (24 h) were selected as the SIC for the compound.…”

Section: Chitosan Solution Preparation and Determination Of Sub-inhibmentioning

confidence: 99%

“…21 The wild type C. jejuni strain was randomly selected from the four strains used in the in vivo trials for gene expression analysis. The strain was cultured with or without SIC of chitosan at 42 ºC in CEB to mid-log phase (8 h) and total RNA was extracted using the RN easy Mini kit (Qiagen, Valencia, CA, USA), followed by complementary DNA synthesis (iScript cDNA synthesis kit, Bio-Rad).…”

Section: Rna Isolation Cdna Synthesis and Real-time Quantitative Pcrmentioning

confidence: 99%

“…SICs of antimicrobials, including antibiotics are known to alter pathophysiology of microbes by modulating gene transcription. 21,[24][25][26] In C. jejuni, the flagellar biosynthesis gene, fliA regulates a large number of genes involved in motility, protein synthesis and colonization. 27 A mutation in fliA has been shown to reduce motility and colonization potential in chicken cecum.…”

Section: Advances In Food Technology and Nutritional Sciencesmentioning

confidence: 99%

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Chitosan Supplementation Reduces Enteric Colonization of Campylobacter jejuni in Broiler Chickens and Down-Regulates Expression of Colonization Genes

Arambel¹,

Donoghue²,

Arsi³

et al. 2015

Adv Food Technol Nutr Sci Open J

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Campylobacter is one of the leading causes of foodborne bacterial gastroenteritis worldwide, and poultry is considered as the most common source of human infections. Campylobacter is prevalent in most poultry flocks and a reduction of Campylobacter in poultry would greatly reduce the risk of campylobacteriosis in humans. Unfortunately, efforts to reduce Campylobacter in pre-harvest poultry have been met with limited success. Preliminary studies with the natural compound, chitosan, demonstrate its ability to kill Campylobacter, in vitro. The purpose of this study was to determine the ability of feed supplemented chitosan to reduce enteric Campylobacter colonization in broiler chickens. Additionally, the effect of chitosan on expression of Campylobacter's chicken colonization genes was investigated using real-time quantitative PCR (RT-qPCR). Because chitosan's antimicrobial properties may vary depending upon its molecular weight, selected doses of three molecular weight chitosans were supplemented in the feed and evaluated for efficacy to reduce Campylobacter in chickens. Three replicate trials were conducted, and in each trial, birds were divided into 10 treatments (n=10 birds/treatment) and were fed 0% (controls), 0.25%, 0.5% or 1% (wt./wt.) of a low, medium or high molecular weight chitosan. Birds were fed treated feed for the duration of the study and orally challenged with a four-strain mixture of wild type C. jejuni on day 6. On day 15, the ceca samples were collected for enumeration of Campylobacter. In all three trials, the 0.5% dose of the medium molecular weight chitosan reduced cecal Campylobacter counts (P<0.05). RTqPCR analysis revealed that chitosan down-regulated the expression of chicken colonization genes as compared to control (P<0.05). These results suggest that supplementation of chitosan in feed is a potential strategy to reduce the enteric colonization of Campylobacter in pre-harvest chickens.

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“…1 Hence, many studies have used biological resources as raw materials for the development and creation of new nontoxic and environmentally friendly wood preservatives as alternatives to CCA and other toxic preservatives. [2][3][4] Rosin, a cheap and important natural raw material, has many excellent characteristics including being antifungal, moisture proof, insulating, conglutinating, emulsifying, and softening. Over the years, rosin was widely used in many fields such as the electronics, medicine, pesticide, and soap industries.…”

Section: Introductionmentioning

confidence: 99%

Quantitative structure–activity relationship of antifungal activity of rosin derivatives

Wang

Nguyen

et al. 2015

Bioorganic & Medicinal Chemistry Letters

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Plant-derived antimicrobials reduce Listeria monocytogenes virulence factors in vitro, and down-regulate expression of virulence genes

Cited by 89 publications

References 51 publications

Eugenol in combination with lactic acid bacteria attenuates Listeria monocytogenes virulence in vitro and in invertebrate model Galleria mellonella

Eugenol in combination with lactic acid bacteria attenuates Listeria monocytogenes virulence in vitro and in invertebrate model Galleria mellonella

Chitosan Supplementation Reduces Enteric Colonization of Campylobacter jejuni in Broiler Chickens and Down-Regulates Expression of Colonization Genes

Quantitative structure–activity relationship of antifungal activity of rosin derivatives

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