Plant-Derived Antifungal Agents: Past and Recent Developments

Vidyasagar, G. M.

doi:10.1007/978-81-322-2782-3_5

Cited by 10 publications

(8 citation statements)

References 156 publications

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“…Microbial diseases have been the leading cause of mortality, and resistant microorganisms threaten public health worldwide (Vidyasagar, 2016;Khameneh et al, 2019;Biharee et al, 2020). Carbapenem-resistant Gram-negative rods are becoming a severe hazard in hospitals, leading to a serious global crisis (Makharita et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Bacterial Outer Membrane Permeability Increase Underlies the Bactericidal Effect of Fatty Acids From Hermetia illucens (Black Soldier Fly) Larvae Fat Against Hypermucoviscous Isolates of Klebsiella pneumoniae

et al. 2022

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Behind expensive treatments, Klebsiella pneumoniae infections account for extended hospitalization’s high mortality rates. This study aimed to evaluate the activity and mechanism of the antimicrobial action of a fatty acid-containing extract (AWME3) isolated from Hermetia illucens (HI) larvae fat against K. pneumoniae subsp. pneumoniae standard NDM-1 carbapenemase-producing ATCC BAA-2473 strain, along with a wild-type hypermucoviscous clinical isolate, strain K. pneumoniae subsp. pneumoniae KPi1627, and an environmental isolate, strain K. pneumoniae subsp. pneumoniae KPM9. We classified these strains as extensive multidrug-resistant (XDR) or multiple antibiotic-resistant (MDR) demonstrated by a susceptibility assay against 14 antibiotics belonging to ten classes of antibiotics. Antibacterial properties of fatty acids extracted from the HI larvae fat were evaluated using disk diffusion method, microdilution, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), half of the inhibitory concentration (MIC50), and bactericidal assays. In addition, the cytotoxocity of AWME3 was tested on human HEK293 cells, and AWME3 lipid profile was determined by gas chromatography-mass spectrometry (GC-MS) analysis. For the first time, we demonstrated that the inhibition zone diameter (IZD) of fatty acid-containing extract (AWME3) of the HI larvae fat tested at 20 mg/ml was 16.52 ± 0.74 and 14.23 ± 0.35 mm against colistin-resistant KPi1627 and KPM9, respectively. It was 19.72 ± 0.51 mm against the colistin-susceptible K. pneumoniae ATCC BAA-2473 strain. The MIC and MBC were 250 μg/ml for all the tested bacteria strains, indicating the bactericidal effect of AWME3. The MIC50 values were 155.6 ± 0.009 and 160.1 ± 0.008 μg/ml against the KPi1627 and KPM9 isolates, respectively, and 149.5 ± 0.013 μg/ml against the ATCC BAA-2473 strain in the micro-dilution assay. For the first time, we demonstrated that AWME3 dose-dependently increased bacterial cell membrane permeability as determined by the relative electric conductivity (REC) of the K. pneumoniae ATCC BAA-2473 suspension, and that none of the strains did not build up resistance to extended AWME3 treatment using the antibiotic resistance assay. Cytotoxicity assay showed that AWME3 is safe for human HEK293 cells at IC50 266.1 μg/ml, while bactericidal for all the strains of bacteria at the same concentration. Free fatty acids (FFAs) and their derivatives were the significant substances among 33 compounds identified by the GC-MS analysis of AWME3. Cis-oleic and palmitoleic acids represent the most abundant unsaturated FAs (UFAs), while palmitic, lauric, stearic, and myristic acids were the most abundant saturated FAs (SFAs) of the AWME3 content. Bactericidal resistant-free AWM3 mechanism of action provides a rationale interpretations and the utility of HI larvae fat to develop natural biocidal resistance-free formulations that might be promising therapeutic against Gram-negative MDR bacteria causing nosocomial infections.

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Section: Introductionmentioning

confidence: 99%

Bacterial Outer Membrane Permeability Increase Underlies the Bactericidal Effect of Fatty Acids From Hermetia illucens (Black Soldier Fly) Larvae Fat Against Hypermucoviscous Isolates of Klebsiella pneumoniae

et al. 2022

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“…Due to the widespread and overuse of limited antifungal drugs, the search for alternatives against C. albicans is ongoing, especially in plants and natural herbs. It is estimated that there are 250,000-500,000 species of plants on earth and only 10% are used by humans [8], which provided 50% of commercially available modern drugs [9]. Plant extract therapies have been utilized and accepted all over the world due to their low side effects.…”

Section: Introductionmentioning

confidence: 99%

“…Plant extract therapies have been utilized and accepted all over the world due to their low side effects. The earliest record of plant medications can be traced back to 2600 BCE in Mesopotamia, Egypt, India, Greece and China, revealing about 300-1000 different drugs [9]. The most common species used by ancient people are algae, bryophytes, pteridophytes and angiosperms [9].…”

Section: Introductionmentioning

confidence: 99%

“…The earliest record of plant medications can be traced back to 2600 BCE in Mesopotamia, Egypt, India, Greece and China, revealing about 300-1000 different drugs [9]. The most common species used by ancient people are algae, bryophytes, pteridophytes and angiosperms [9]. Different geographical locations also have a big impact on the development of herbal drug systems and the availability of plant resources.…”

Section: Introductionmentioning

confidence: 99%

“…Kier and coworkers describe that the highest diversity of plants may be found in the Neotropic (central and south America) and the Asia-Pacific region (China, India, USA, Australia), and lower diversities in Africa and on oceanic islands [10]. Traditionally, the majority of medicinal plants are found in India and China, while Europe and the USA have developed fewer sources [9]. The diversity of plants provides a wide range of important sources of biologically active molecules with enormous potential antifungal properties, such as phenols, tannins, terpenoids and alkaloids [9].…”

Section: Introductionmentioning

confidence: 99%

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Herbal Extracts with Antifungal Activity against Candida albicans: A Systematic Review

Hsu¹

2021

MRMC

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In the era of antimicrobial resistance, fungal pathogens are not an exception. Several strategies, including antimicrobial stewardship programs and high throughput screening of new drugs, are being implemented. Several recent studies have demonstrated the effectiveness of plant compounds with antifungal activity. In this systematic review, we examine the use of natural compounds as a possible avenue to fight fungal infections produced by Candida albicans, the most common human fungal pathogen. Electronic literature searches were conducted through PubMed/MEDLINE, Cochrane, and Science Direct limited to the 5 years. A total of 131 articles were included, with 186 plants extracts evaluated. Although the majority of the natural extracts exhibited antifungal activities against C. albicans (both in vivo and in vitro), the strongest antifungal activity was obtained from Lawsonia inermis, Pelargonium graveolens, Camellia sinensis, Mentha piperita, and Citrus latifolia. The main components with proven antifungal activities were phenolic compounds such as gallic acid, thymol, and flavonoids (especially catechin), polyphenols such as tannins, terpenoids and saponins. The incorporation of nanotechnology greatly enhances the antifungal properties of these natural compounds. Further research is needed to fully characterize the composition of all herbal extracts with antifungal activity as well as the mechanisms of action of the active compounds.

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Phytopharmaceuticals for the Management of Fungal Infections

Huanbutta¹,

Sangnim²

2022

Herbal Drugs for the Management of Infectious Diseases

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Plant-Derived Antifungal Agents: Past and Recent Developments

Cited by 10 publications

References 156 publications

Bacterial Outer Membrane Permeability Increase Underlies the Bactericidal Effect of Fatty Acids From Hermetia illucens (Black Soldier Fly) Larvae Fat Against Hypermucoviscous Isolates of Klebsiella pneumoniae

Bacterial Outer Membrane Permeability Increase Underlies the Bactericidal Effect of Fatty Acids From Hermetia illucens (Black Soldier Fly) Larvae Fat Against Hypermucoviscous Isolates of Klebsiella pneumoniae

Herbal Extracts with Antifungal Activity against Candida albicans: A Systematic Review

Phytopharmaceuticals for the Management of Fungal Infections

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