Plant defensin expression triggered by fungal pathogen invasion depends on EDR1 protein kinase and ORA59 transcription factor in <i>Arabidopsis thaliana</i>

Kosaka, Atsuko; Suemoto, Haruka; Singkaravanit-Ogawa, Suthitar; Takano, Yoshitaka

doi:10.1080/15592324.2020.1823120

Cited by 8 publications

(7 citation statements)

References 25 publications

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“…Bars, 200 lm. at 17 hpi in pen2, which is defective in pre-invasive resistance against Ctro, but not in WT Col-0, indicating strong induction of PDF1.2a by Ctro invasion, consistent with our recent report (Kosaka et al, 2020). Notably, we found that PDF1.2a expression at 17 hpi with Ctro was completely abolished in both clf mutants (Figure 5a).…”

Section: Clf Contributes To the Induced Expression Of Multiple Plant Defensin Genes By Positively Controlling Ora59 Expression Upon Pathosupporting

confidence: 92%

“…These results indicate that CLF is involved in the induced expression of multiple PDF genes in both pre-invasive and postinvasive resistance against Ctro. We have recently reported that ORA59 and EDR1 are required for the induced expression of PDF genes upon invasion of Ctro (Kosaka et al, 2020). In agreement with this, the Ctro invasion-triggered induction of PDF1.2a at 17 hpi was eliminated in both edr1 and pen2 ora59 mutants (Figure 5a) (note that the ora59 single mutant is not defective in pre-invasive resistance against Ctro).…”

Section: Clf Contributes To the Induced Expression Of Multiple Plant Defensin Genes By Positively Controlling Ora59 Expression Upon Pathosupporting

confidence: 79%

“…We also found that CLF positively contributes to the induced expression of PDF genes and the Octadecanoidresponsive Arabidopsis 59 (ORA59) gene, which is required for PDF expression upon Ctro inoculation (Kosaka et al, 2020). We finally reveal that concomitant SEP3 repression and ORA59 induction via CLF are critical for Arabidopsis leaf immunity.…”

Section: Introductionmentioning

confidence: 70%

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Arabidopsis CURLY LEAF functions in leaf immunity against fungal pathogens by concomitantly repressing SEPALLATA3 and activating ORA59

et al. 2021

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Arabidopsis non-host resistance against non-adapted fungal pathogens including Colletotrichum fungi consists of pre-invasive and post-invasive immune responses. Here we report that non-host resistance against non-adapted Colletotrichum spp. in Arabidopsis leaves requires CURLY LEAF (CLF), which is critical for leaf development, flowering and growth. Microscopic analysis of pathogen behavior revealed a requirement for CLF in both pre-and post-invasive non-host resistance. The loss of a functional SEPALLATA3 (SEP3) gene, ectopically expressed in clf mutant leaves, suppressed not only the defect of the clf plants in growth and leaf development but also a defect in non-host resistance against the non-adapted Colletotrichum tropicale. However, the ectopic overexpression of SEP3 in Arabidopsis wild-type leaves did not disrupt the non-host resistance. The expression of multiple plant defensin (PDF) genes that are involved in non-host resistance against C. tropicale was repressed in clf leaves. Moreover, the Octadecanoid-responsive Arabidopsis 59 (ORA59) gene, which is required for PDF expression, was also repressed in clf leaves. Notably, when SEP3 was overexpressed in the ora59 mutant background, C. tropicale produced clear lesions in the inoculated leaves, indicating an impairment in non-host resistance. Furthermore, ora59 plants overexpressing SEP3 exhibited a defect in leaf immunity to the adapted Colletotrichum higginsianum. Since the ora59 plants overexpressing SEP3 did not display obvious leaf curling or reduced growth, in contrast to the clf mutants, these results strongly suggest that concomitant SEP3 repression and ORA59 induction via CLF are required for Arabidopsis leaf immunity to Colletotrichum fungi, uncoupled from CLF's function in growth and leaf development.

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Section: Clf Contributes To the Induced Expression Of Multiple Plant Defensin Genes By Positively Controlling Ora59 Expression Upon Pathosupporting

confidence: 92%

Section: Clf Contributes To the Induced Expression Of Multiple Plant Defensin Genes By Positively Controlling Ora59 Expression Upon Pathosupporting

confidence: 79%

Section: Introductionmentioning

confidence: 70%

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Arabidopsis CURLY LEAF functions in leaf immunity against fungal pathogens by concomitantly repressing SEPALLATA3 and activating ORA59

et al. 2021

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“…For example, foliar application of the endophytic fungus Colletotrichum tropicale reduced Phytophthora infection in the Cacao tree ( Theobroma cacao) [ 39 ]. The reduction in Phytophthora infection could be attributed to the induction of defense-related pathways, as inoculation with C. tropicale triggered several components of the ethylene (ET) defense pathway and induced several disease-related genes in T. cacao and Arabidopsis thaliana [ 40 , 41 ].…”

Section: Endophytes Modulate Plant Defense Responsesmentioning

confidence: 99%

Endophyte-Mediated Stress Tolerance in Plants: A Sustainable Strategy to Enhance Resilience and Assist Crop Improvement

Kamran

Imran

Ahmed

et al. 2022

Cells

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Biotic and abiotic stresses severely affect agriculture by affecting crop productivity, soil fertility, and health. These stresses may have significant financial repercussions, necessitating a practical, cost-effective, and ecologically friendly approach to lessen their negative impacts on plants. Several agrochemicals, such as fertilizers, pesticides, and insecticides, are used to improve plant health and protection; however, these chemical supplements have serious implications for human health. Plants being sessile cannot move or escape to avoid stress. Therefore, they have evolved to develop highly beneficial interactions with endophytes. The targeted use of beneficial plant endophytes and their role in combating biotic and abiotic stresses are gaining attention. Therefore, it is important to experimentally validate these interactions and determine how they affect plant fitness. This review highlights research that sheds light on how endophytes help plants tolerate biotic and abiotic stresses through plant–symbiont and plant–microbiota interactions. There is a great need to focus research efforts on this vital area to achieve a system-level understanding of plant–microbe interactions that occur naturally.

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“…For instance, a recent study reported that Arabidopsis CLF, a PRC2 component, is positively required for the expression of ORA59 , a stress-responsive AP2/ERF transcription factor. The reduced ORA59 expression in a clf mutant results in at least a partial defect in leaf immunity because of the low expression of defense-related genes such as PDF1.2a ( Kosaka et al., 2020 ; Singkaravanit-Ogawa et al., 2021 ). On the contrary, in our study using B. rapa , ORA59 was upregulated in the brclf mutant compared with that in the R-o-18 wild type; furthermore, H3K27me3 was densely enriched in the ORA59 chromatin region ( Figure 4B ; Supplementary Figure S9 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and epigenomic analyses revealed that polycomb repressive complex 2 regulates not only developmental but also stress responsive metabolism in Brassica rapa

Nugroho

Kim²,

Lee³

et al. 2023

Front. Plant Sci.

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Polycomb group proteins (PcG) play a crucial role in developmental programs in eukaryotic organisms, including plants. PcG-mediated gene repression is achieved by epigenetic histone modification on target chromatins. Loss of PcG components leads to severe developmental defects. CURLY LEAF (CLF), a PcG component in Arabidopsis, catalyzes the trimethylation of histone H3 on lysine 27 (H3K27me3), a repressive histone mark in numerous genes in Arabidopsis. In this study, we isolated a single homolog of Arabidopsis CLF, namely, BrCLF, in Brassica rapa ssp. trilocularis. Transcriptomic analysis revealed that BrCLF participated in B. rapa developmental processes, such as seed dormancy, leaf and flower organ development, and floral transition. BrCLF was also involved in stress signaling and stress-responsive metabolism, such as aliphatic and indolic glucosinolate metabolism in B. rapa. Epigenome analysis showed that H3K27me3 was substantially enriched in genes related to these developmental and stress-responsive processes. Thus, this study provided a basis for elucidating the molecular mechanism of the PcG-mediated regulation of development and stress responses in B. rapa.

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Plant defensin expression triggered by fungal pathogen invasion depends on EDR1 protein kinase and ORA59 transcription factor in Arabidopsis thaliana

Cited by 8 publications

References 25 publications

Arabidopsis CURLY LEAF functions in leaf immunity against fungal pathogens by concomitantly repressing SEPALLATA3 and activating ORA59

Arabidopsis CURLY LEAF functions in leaf immunity against fungal pathogens by concomitantly repressing SEPALLATA3 and activating ORA59

Endophyte-Mediated Stress Tolerance in Plants: A Sustainable Strategy to Enhance Resilience and Assist Crop Improvement

Transcriptomic and epigenomic analyses revealed that polycomb repressive complex 2 regulates not only developmental but also stress responsive metabolism in Brassica rapa

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