Plant cytoskeletons contain intermediate filament-related proteins

Miller, Christopher C.J.; Duckett, Jeffrey G.; Downes, Malcolm J.; Cowell, Ian G.; Dowding, Alan J.; Virtanen, Ismo; Anderton, Brian H.

doi:10.1042/bst0130960

Cited by 7 publications

(2 citation statements)

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“…Moreover, their integrated nature and inherent links with the nuclear skeleton underlie direct impacts on expression of the eukaryotic genome (Ingber, 1993;Ingber et al, 1994;Chicurel et al, 1998). The hunt for IFs in plants is, after the first promising results (Dawson et al, 1985;Miller et al, 1985;Mc-Nulty and Saunders, 1992), still an open race (Menzel, 1993) and it seems that, as with yeast cells, plant cells rely only on MTs and AFs.…”

Section: Introductionmentioning

confidence: 99%

Actin cytoskeleton in plants: From transport networks to signaling networks

Volkmann

Baluška

1999

Microsc. Res. Tech.

144

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The plant actin cytoskeleton is characterized by a high diversity in regard to gene families, isoforms, and degree of polymerization. In addition to the most abundant F‐actin assemblies like filaments and their bundles, G‐actin obviously assembles in the form of actin oligomers composed of a few actin molecules which can be extensively cross‐linked into complex dynamic meshworks. The role of the actomyosin complex as a force generating system — based on principles operating as in muscle cells — is clearly established for long‐range mass transport in large algal cells and specialized cell types of higher plants. Extended F‐actin networks, mainly composed of F‐actin bundles, are the structural basis for this cytoplasmic streaming of high velocities On the other hand, evidence is accumulating that delicate meshworks built of short F‐actin oligomers are critical for events occurring at the plasma membrane, e.g., actin interventions into activities of ion channels and hormone carriers, signaling pathways based on phospholipids, and exo‐ and endocytotic processes. These unique F‐actin arrays, constructed by polymerization‐depolymerization processes propelled via synergistic actions of actin‐binding proteins such as profilin and actin depolymerizing factor (ADF)/cofilin are supposed to be engaged in diverse aspects of plant morphogenesis. Finally, rapid rearrangements of F‐actin meshworks interconnecting endocellular membranes turn out to be especially important for perception‐signaling purposes of plant cells, e.g., in association with guard cell movements, mechano‐ and gravity‐sensing, plant host–pathogen interactions, and wound‐healing. Microsc. Res. Tech. 47:135–154, 1999. © 1999 Wiley‐Liss, Inc.

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Section: Introductionmentioning

confidence: 99%

Actin cytoskeleton in plants: From transport networks to signaling networks

Volkmann

Baluška

1999

Microsc. Res. Tech.

144

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“…Dawso [18]. Later, Su et al, using immunogold labeling with monoclonal antibody of cytokeratin from animal cells, found a keratin intermediate filament-like system in maize protoplasts [9].…”

Section: Dmentioning

confidence: 99%

Cytoskeletal arrangement and its intercellular connection in wheat young leaf cells

Wei

Ling

1993

Cell Res

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By using the techniques of partial digestion of cell wall and selective extraction, we examined the cytoskeleton of wheat young leaf cells under scanning electron microscope(SEM). A 3-dimensional cytoskeletal system, showing some new features, was observed. The cortical network located beneath the cross wall was an anastomosing organization. The association of nucleus with the cell wall by some skeletal filaments was also found. It is noticeable that there were cytoskeletal filaments, which passed through cell wall and connected together with cytoskeletal arrays of adjacent cells. Thus, it is possible that an integral skeletal network existed within the young leaf tissue of wheat.

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Monoclonal antibodies against plant proteins recognise animal intermediate filaments

Parke

Miller

Cowell

et al. 1987

Cell Motil. Cytoskeleton

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Four monoclonal antibodies were raised against polypeptides present in a high-salt detergent-insoluble fraction from cells of Chlamydomonas reinhardtii. Indirect immunofluorescence microscopy of fibroblasts and epithelial cells grown in culture using these plant antibodies revealed staining arrays identical to those obtained with well characterised antibodies to animal intermediate filaments. Immunofluorescence microscopy of Chlamydomonas with these monoclonal antibodies and a monoclonal antibody that recognises all animal intermediate filaments (anti-IFA) gave a diffuse, patchy cytoplasmic staining pattern. Both the plant antibodies and anti-IFA stained interphase onion root tip cells in a diffuse perinuclear pattern. In metaphase through to telophase, the labelling patterns colocalised with those of microtubules. Labelling of the phragmoplast was also detected but not staining of the preprophase band. On Western blots of various animal cell lines and tissues, all the antibodies labelled known intermediate filament proteins. On Western blots of whole Chlamydomonas proteins, all the antibodies labelled a broad band in the 57,000 Mr range, and three antibodies labelled bands around 66,000 and 140,000 Mr but with variable intensities. On Western blots of whole onion root tip proteins, all the antibodies labelled 50,000 Mr (two to three bands) polypeptides and a diffuse band around 60,000 Mr and three of the antibodies also labelled several polypeptides in the 90,000-200,000 Mr range. The consistent labelling of these different bands by several different monoclonal antibodies recognising animal intermediate filaments makes these polypeptides putative plant intermediate filament proteins.

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Plant cytoskeletons contain intermediate filament-related proteins

Cited by 7 publications

References 0 publications

Actin cytoskeleton in plants: From transport networks to signaling networks

Actin cytoskeleton in plants: From transport networks to signaling networks

Cytoskeletal arrangement and its intercellular connection in wheat young leaf cells

Monoclonal antibodies against plant proteins recognise animal intermediate filaments

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