Plant cysteine proteinases: Evaluation of the pharmacological activity

Effects of bromelain on cellular characteristics and expression of selected genes in canine in vitro chondrocyte culture

“…Nevertheless, there was a different difference between the effect of bromelain versus other proteinases such as collagenase, cathepsin or papain, which had an adverse effect on the cartilage matrix component and matrix synthesis (van Osch et al, 1998;Yang et al, 2000;Cawston and Wilson, 2006) while bromelain showed no harmful effects. It is suspected that bromelain contains several distinct cysteine proteinases that have distinct amino acid sequences, as well as differences in proteolytic specificity and sensitivity to inactivation (Hale et al, 2005;Salas et al, 2008).…”

Affinity Chromatography as a Key Tool to Purify Protein Protease Inhibitors from Plants

“…Plant proteases are involved in protein processing (activation, maturation, degradation) in virtually all aspect of the physiology and development of plant (Brzin & Kidric, 1995;Grudkowska & Zagdanska, 2004;Salas et al, 2008). Other important cysteine proteases include most of the lysosomal cathepsins, the calpains (a cytosolic "calcium-activated neutral protease"), caspases (essential for apoptosis process) Xu & Chye, 1999) and several viral and parasite proteases (Fan & Wu, 2005;Otto & Schirmeister, 1997;Rawlings et al, 2010;Turk et al, 2000).…”

Plant Proteinaceous α-Amylase and Proteinase Inhibitors and Their Use in Insect Pest Control

“…There have been considerable number of reviews on plant PIs describing their classification (Turra et al, 2011;Volpicella et al, 2011), biochemical and structural properties (Antao and Malcata, 2005;Bateman and James, 2011;Oliva et al, 2011), their role in plant physiology (Schaller, 2004;Salas et al, 2008;Roberts and Hejgaard, 2008), insect-plant co-evolution (Jongsma and Beekwilder, 2011), and their application in different areas including pest control (Lawrence and Koundal, 2002;, nutritional (Clemente et al, 2011) as well as pharmaceutical (Gomes et al, 2011) applications.…”