Plant Compartments Shape the Assembly and Network of Vallisneria natans-Associated Microorganisms

Abstract

The submerged plant Vallisneria natans can provide an attachment matrix and habitat for diverse microorganisms and plays an important role in maintaining the structure and function of the shallow lake ecosystem. However, little is known about how V. natans-related microorganism components, especially bacteria, adapt to specific plant compartments. In this study, we investigated the assembly and network of bacterial communities living in different plant compartments (sediment, rhizosphere, rhizoplane, root endosphere, and leaf endosphere) associated with V. natans by 16S rRNA gene sequencing. The results showed that the diversity and network complexity of the bacterial community in the sediment was significantly higher than that in other plant compartments. The bacterial community composition showed that the dominant phyla were Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, Desulfobacterota, and Chloroflexi, among which Proteobacteria were extremely abundant in all samples, and there were notable differences in bacterial community composition related to plant compartments. Different networks based on sediment and plant compartments showed distinct co-occurrence patterns and exhibited distinct topological features. Additionally, functional predictions from FAPROTAX indicate that the predominant biogeochemical cycle function of the V. natans-related bacterial community is to participate in the carbon and nitrogen cycle. These results strongly suggested how the microbial community adapted to different plant compartments and provided theoretical and technical data for isolating beneficial bacteria from macrophytes in the future.

Linking ecological niches to bacterial community structure and assembly in polluted urban aquatic ecosystems

“…By attaching to the leaves and roots of submerged plants, microorganisms can exchange nutrients and signals with plants, significantly influencing the ecological processes within the soil and water columns. For instance, Wan et al (2023) documented significant variations in the bacterial communities in plant compartments, such as the rhizosphere, rhizoplane, root endosphere, and leaf endosphere, of the submerged plant Vallisneria natans. Similar spatial heterogeneity has been documented in other aquatic plants such as Cymodocea nodosa and Myriophyllum verticillatum (Zhu et al, 2021;Banister et al, 2022).…”