Plant chitinase responses to different metal-type stresses reveal specificity

Mészáros, Patrik; Rybanský, Ľubomír; Spieß, Nadine; Socha, Peter; Kuna, Roman; Libantová, Jana; Moravčíková, Jana; Piršelová, Beáta; Hauptvogel, Pavol; Matušíková, Ildikó

doi:10.1007/s00299-014-1657-9

Cited by 23 publications

(13 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Quite fittingly, we also observed that chitinases were increased in roots of both treatments. These, fungal and bacterial cell wall degenerating enzymes, are thought to be induced during HM stress by changes in cellular oxidative status (Javed et al, 2013) as they contribute to water retention, decrease cell wall elasticity and permeability for metals (Kasprzewska, 2003; Mészáros et al, 2014). However, in addition, we found another cell wall associated enzyme, a ß-expansin (I1MV71) to be highly responsive to tungsten.…”

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of Tungsten Toxicity Differ for Glycine max Depending on Nitrogen Regime

et al. 2019

View full text Add to dashboard Cite

Tungsten (W) finds increasing application in military, aviation and household appliance industry, opening new paths into the environment. Since W shares certain chemical properties with the essential plant micronutrient molybdenum (Mo), it is proposed to inhibit enzymatic activity of molybdoenzymes [e.g., nitrate reductase (NR)] by replacing the Mo-ion bound to the co-factor. Recent studies suggest that W, much like other heavy metals, also exerts toxicity on its own. To create a comprehensive picture of tungsten stress, this study investigated the effects of W on growth and metabolism of soybean ( Glycine max ), depending on plant nitrogen regime [nitrate fed (N fed) vs. symbiotic N 2 fixation (N fix)] by combining plant physiological data (biomass production, starch and nutrient content, N 2 fixation, nitrate reductase activity) with root and nodule proteome data. Irrespective of N regime, NR activity and total N decreased with increasing W concentrations. Nodulation and therefore also N 2 fixation strongly declined at high W concentrations, particularly in N fix plants. However, N 2 fixation rate (g N fixed g −1 nodule dwt) remained unaffected by increasing W concentrations. Proteomic analysis revealed a strong decline in leghemoglobin and nitrogenase precursor levels (NifD), as well as an increase in abundance of proteins involved in secondary metabolism in N fix nodules. Taken together this indicates that, in contrast to the reported direct inhibition of NR, N 2 fixation appears to be indirectly inhibited by a decrease in nitrogenase synthesis due to W induced changes in nodule oxygen levels of N fix plants. Besides N metabolism, plants exhibited a strong reduction of shoot (both N regimes) and root (N fed only) biomass, an imbalance in nutrient levels and a failure of carbon metabolic pathways accompanied by an accumulation of starch at high tungsten concentrations, independent of N-regime. Proteomic data (available via ProteomeXchange with identifier PXD010877) demonstrated that the response to high W concentrations was independent of nodule functionality and dominated by several peroxidases and other general stress related proteins. Based on an evaluation of several W responsive proteotypic peptides, we identified a set of protein markers of W stress and possible targets for improved stress tolerance.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of Tungsten Toxicity Differ for Glycine max Depending on Nitrogen Regime

et al. 2019

View full text Add to dashboard Cite

show abstract

“…; Mészáros et al . ). In general, chitinases are ROS‐regulated and thus the reported increased abundance of chitinases is not specific to Cd and rather reflects the general stress response of M. sativa (Metwally et al .…”

Section: Discussionmentioning

confidence: 99%

Long‐term cadmium exposure influences the abundance of proteins that impact the cell wall structure in Medicago sativa stems

et al. 2018

View full text Add to dashboard Cite

Cadmium (Cd) is a non‐essential, toxic heavy metal that poses serious threats to both ecosystems and human health. Plants employ various cellular and molecular mechanisms to minimise the impact of Cd toxicity and cell walls function as a defensive barrier during Cd exposure.In this study, we adopted a quantitative gel‐based proteomic approach (two‐dimensional difference gel electrophoresis) to investigate changes in the abundance of cell wall and soluble proteins in stems of Medicago sativa L. upon long‐term exposure to Cd (10 mg·Cd·kg−1 soil as CdSO 4). Obtained protein data were complemented with targeted gene expression analyses.Plants were affected by Cd exposure at an early growth stage but seemed to recover at a more mature stage as no difference in biomass was observed. The accumulation of Cd was highest in roots followed by stems and leaves. Quantitative proteomics revealed a changed abundance for 179 cell wall proteins and 30 proteins in the soluble fraction upon long‐term Cd exposure. These proteins are involved in cell wall remodelling, defence response, carbohydrate metabolism and promotion of the lignification process.The data indicate that Cd exposure alters the cell wall proteome and underline the role of cell wall proteins in defence against Cd stress. The identified proteins are linked to alterations in cell wall structure and lignification process in stems of M. sativa, underpinning the function of the cell wall as an effective barrier against Cd stress.

show abstract

“…Apparently, they might provide advantages for plants during heavy metal stress. Similarly, Meszaros 26 and Bekesiova 23 found that heavy metal stress could induce more chitinases in plants. Dana et al reported overexpressing chitinases tobacco showed exhibit enhanced levels of resistance to biotic and abiotic stress 27 .…”

Section: Discussionmentioning

confidence: 88%

Identification of key genes and modules in response to Cadmium stress in different rice varieties and stem nodes by weighted gene co-expression network analysis

Wang

Zeng

Song

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Soil cadmium (Cd) pollution threatens food safety. This study aimed to identify genes related to Cd accumulation in rice. Low-(Shennong 315, short for S315) and high-(Shendao 47, short for S47) Cd-accumulative rice cultivars were incubated with CdCl 2 •2.5H 2 O. RNA-seq and weighted gene coexpression network analysis (WGCNA) were performed to identify the modules and genes associated with Cd-accumulative traits of rice. After Cd stress treatment, the Cd content in various tissues of S315 was significantly higher than that of S47. In the stem nodes, the Cd distribution results of the two varieties indicated that the unelongated nodes near the root (short for node A) had a stronger ability to block Cd transfer upwards than the panicle node (short for node B). Cd stress induced huge changes in gene expression profiles. After analyzing the differentially expressed genes (DEGs) in significantly correlated WGCNA modules, we found that genes related to heavy metal transportation had higher expression levels in node A than that in node B, such as Copper transporter 6 (OS04G0415600), Zinc transporter 10 (OS06G0566300), and some heavy-metal associated proteins (OS11G0147500, OS03G0861400, and OS10G0506100). In the comparison results between S315 and S47, the expression of chitinase (OS03G0679700 and OS06G0726200) was increased by Cd treatment in S315. In addition, OsHSPs (OS05G0460000, OS08G0500700), OsHSFC2A (OS02G0232000), and OsDJA5 (OS03G0787300) were found differentially expressed after Cd treatment in S315, but changed less in S47. In summary, different rice varieties have different processes and intensities in response to Cd stress. The node A might function as the key tissue for blocking Cd upward transport into the panicle via vigorous processes, including of heavy metal transportation, response to stress, and cell wall. Heavy metal cadmium (Cd) contaminates a large area of rice (Oryza sativa), which is one of the largest food crops in China and worldwide 1. Cd pollution causes irreversible soil problems in China. Various technologies and great efforts have been made in the treatment of Cd pollution and restoration of Cd contaminated soils, but the little effect has been achieved. Screening and breeding for low Cd-accumulative cultivars could relieve Cd-induced pressure in cropping system 2,3. Many plant species with low cadmium accumulation have been proposed and widely promoted in scientific research and cropping 2,4. Consequently, identifying genetic targets that can be used to reduce cadmium accumulation in crops is important for plant breeding 5-8. Many genes related to Cd stress have been identified. For instance, the expression of Arabidopsis PLANT DEFENSIN 2 (AtPDF2.5) can promote Cd accumulation in Arabidopsis roots 5. Tang et al. reported that the knockout of OsNramp5 (a magnesium ion (Mg2+) and Cd transporter and a Mn and Cd uptake gene) in rice reduced the accumulation of Cd in rice 8. The comparative transcriptome analysis of low and high Cd accumulation genotypes has been performed on Brassica Chinen...

show abstract

Plant chitinase responses to different metal-type stresses reveal specificity

Cited by 23 publications

References 55 publications

Molecular Mechanisms of Tungsten Toxicity Differ for Glycine max Depending on Nitrogen Regime

Molecular Mechanisms of Tungsten Toxicity Differ for Glycine max Depending on Nitrogen Regime

Long‐term cadmium exposure influences the abundance of proteins that impact the cell wall structure in Medicago sativa stems

Identification of key genes and modules in response to Cadmium stress in different rice varieties and stem nodes by weighted gene co-expression network analysis

Contact Info

Product

Resources

About