Plant Cells under Attack: Unconventional Endomembrane Trafficking during Plant Defense

Ruano, Guillermo; Scheuring, David

doi:10.3390/plants9030389

Cited by 29 publications

(23 citation statements)

References 146 publications

(163 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The total amount of pectins (CDTA and Na 2 CO 3 extracted polysaccharides) increased upon the Pph inoculation ( Figure 5 ). Indeed, the higher HG methylation degree observed, as revealed by immunolabeling in these fractions ( Figure 8 ), would suggest a HG synthesis increase after the infection, as HG methyl-esterification only occurs in Golgi apparatus [ 77 , 78 , 79 ], and/or a decrease of pectin methyl esterase activity after the Pph infection, as this is altered in other pathosystems [ 27 , 80 ]. Besides, the Ara and Xyl variation in the KII fraction suggest an increment of AX in tightly cross-linked hemicelluloses.…”

Section: Discussionmentioning

confidence: 99%

Immune Priming Triggers Cell Wall Remodeling and Increased Resistance to Halo Blight Disease in Common Bean

Rubia

Mélida

Centeno

et al. 2021

Plants

View full text Add to dashboard Cite

The cell wall (CW) is a dynamic structure extensively remodeled during plant growth and under stress conditions, however little is known about its roles during the immune system priming, especially in crops. In order to shed light on such a process, we used the Phaseolus vulgaris-Pseudomonas syringae (Pph) pathosystem and the immune priming capacity of 2,6-dichloroisonicotinic acid (INA). In the first instance we confirmed that INA-pretreated plants were more resistant to Pph, which was in line with the enhanced production of H2O2 of the primed plants after elicitation with the peptide flg22. Thereafter, CWs from plants subjected to the different treatments (non- or Pph-inoculated on non- or INA-pretreated plants) were isolated to study their composition and properties. As a result, the Pph inoculation modified the bean CW to some extent, mostly the pectic component, but the CW was as vulnerable to enzymatic hydrolysis as in the case of non-inoculated plants. By contrast, the INA priming triggered a pronounced CW remodeling, both on the cellulosic and non-cellulosic polysaccharides, and CW proteins, which resulted in a CW that was more resistant to enzymatic hydrolysis. In conclusion, the increased bean resistance against Pph produced by INA priming can be explained, at least partially, by a drastic CW remodeling.

show abstract

Section: Discussionmentioning

confidence: 99%

Immune Priming Triggers Cell Wall Remodeling and Increased Resistance to Halo Blight Disease in Common Bean

Rubia

Mélida

Centeno

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…Endoplasmic reticulum (ER)-bodies accumulate defense proteins (Chuberre et al, 2018 ) and the ER is now recognizably a hub to sort proteins to plastids and mitochondria (Bellucci et al, 2018 ). Activation of endomembrane trafficking associated to an increase in salicylic acid levels during plant defense has also been reported (Ruano and Scheuring, 2020 ). Although appealing, this hypothesis clearly requires further experimental evidence.…”

Section: Discussionmentioning

confidence: 83%

Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi

et al. 2021

View full text Add to dashboard Cite

Allene oxide synthase (AOS) is a key enzyme of the jasmonic acid (JA) signaling pathway. The AOS gene was previously found to be upregulated in an Asian chestnut species resistant to infection by the oomycete Phytophthora cinnamomi (Castanea crenata), while lower expression values were detected in the susceptible European chestnut (Castanea sativa). Here, we report a genetic and functional characterization of the C. crenata AOS (CcAOS) upon its heterologous gene expression in a susceptible ecotype of Arabidopsis thaliana, which contains a single AOS gene. It was found that Arabidopsis plants expressing CcAOS delay pathogen progression and exhibit more vigorous growth in its presence. They also show upregulation of jasmonic acid and salicylic acid-related genes. As in its native species, heterologous CcAOS localized to plastids, as revealed by confocal imaging of the CcAOS-eGFP fusion protein in transgenic Arabidopsis roots. This observation was confirmed upon transient expression in Nicotiana benthamiana leaf epidermal cells. To further confirm a specific role of CcAOS in the defense mechanism against the pathogen, we performed crosses between transgenic CcAOS plants and an infertile Arabidopsis AOS knockout mutant line. It was found that plants expressing CcAOS exhibit normal growth, remain infertile but are significantly more tolerant to the pathogen than wild type plants. Together, our results indicate that CcAOS is an important player in plant defense responses against oomycete infection and that its expression in susceptible varieties may be a valuable tool to mitigate biotic stress responses.

show abstract

“…Rhi-milR-13 regulates Osa-vacuolar-sorting receptor (VSR) of rice which is responsible for targeting defense-related soluble proteins to the vacuole. Plant vacuoles play an important role in defense by releasing hydrolytic enzymes and antimicrobial compounds upon pathogen infection that leads to programmed cell death due to hypersensitive response [ 48 ]. Suppression of rice VSR by R. solani milRNA might suppress the plant defense by inhibiting the hypersensitive response.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Expression Profiling of Small RNAs in Indian Strain of Rhizoctonia solani AG1-1A Reveals Differential Regulation of milRNAs during Pathogenesis and Crosstalk of Gene Regulation

Prathi

Rani

Balachandran

et al. 2021

JoF

View full text Add to dashboard Cite

Rhizoctonia solaniAG1-1A is a necrotrophic fungus that causes sheath blight disease in rice. The reliable resistant source against this phytopathogenic fungus is not available in the gene pool of rice. Better understanding of pathogen genomics and gene regulatory networks are critical to devise alternate strategies for developing resistance against this noxious pathogen. In this study, miRNA-like RNAs (milRNAs) of an Indian strain of R. solani were identified by deep sequencing of small RNAs. We identified 128 known and 22 novel milRNAs from 20,963,123 sequence reads. These milRNAs showed 1725 target genes in the fungal genome which include genes associated with growth, development, pathogenesis and virulence of R. solani. Notably, these fungal milRNAs showed their target genes in host (rice) genome also which were later verified by qRT-PCR. The host target genes are associated with auxin metabolism, hypersensitive response, defense genes, and genes related to growth and development of rice. Osa-vacuolar-sorting receptor precursor: Rhi-milR-13, Osa-KANADI1:Rhi-milR-124, Osa-isoflavone reductase: Rhi-milR-135, Osa-nuclear transcription factor Y:Rhi-milR-131, Osa-NB-ARC domain containing protein: Rhi-milR-18, and Osa-OsFBX438: Rhi-milR-142 are notable potential regulons of host target genes: fungal milRNAs that need to be investigated for better understanding of the crosstalk of RNAi pathways between R. solani and rice. The detailed expression analysis of 17 milRNAs by qRT-PCR was analysed during infection at different time points of inoculation, at different growth stages of the host, in four different genotypes of the host, and also in four different strains of fungi which revealed differential regulation of milRNAs associated with pathogenesis and virulence. This study highlights several important findings on fungal milRNAs which need to be further studied and characterized to decipher the gene expression and regulation of this economically important phytopathogen.

show abstract

Plant Cells under Attack: Unconventional Endomembrane Trafficking during Plant Defense

Cited by 29 publications

References 146 publications

Immune Priming Triggers Cell Wall Remodeling and Increased Resistance to Halo Blight Disease in Common Bean

Immune Priming Triggers Cell Wall Remodeling and Increased Resistance to Halo Blight Disease in Common Bean

Expression of Castanea crenata Allene Oxide Synthase in Arabidopsis Improves the Defense to Phytophthora cinnamomi

Genome-Wide Expression Profiling of Small RNAs in Indian Strain of Rhizoctonia solani AG1-1A Reveals Differential Regulation of milRNAs during Pathogenesis and Crosstalk of Gene Regulation

Contact Info

Product

Resources

About