Plant cell walls to ethanol

Jordan, Douglas B.; Bowman, Michael J.; Braker, Jay D.; Dien, Bruce S.; Hector, Ronald E.; Lee, Charles C.; Mertens, Jeffrey A.; Wagschal, Kurt

doi:10.1042/bj20111922

Cited by 167 publications

(126 citation statements)

References 191 publications

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“…ABFs are important in efficient and complete degradation of plant cell wall hemicelluloses and pectin and application of ABFs has potential economic and environmental benefits in biorefineries for production of second generation bioethanol (Jordan et al, 2012). The hemicellulose fraction of feed-stock as corn and wheat straw can be highly substituted with Araf ( Fig.…”

Section: Biorefineriesmentioning

confidence: 99%

GH62 arabinofuranosidases: Structure, function and applications

Wilkens

Andersen

Dumon

et al. 2017

Biotechnology Advances

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Motivated by industrial demands and ongoing scientific discoveries continuous efforts are made to identify and create improved biocatalysts dedicated to plant biomass conversion. --1,3 arabinofuranosyl specific -L-arabinofuranosidases (EC 3.2.1.55) are debranching enzymes catalyzing hydrolytic release -L-arabinofuranosyl residues, which decorate xylan or arabinan backbones in lignocellulosic and pectin constituents of plant cell walls. The CAZy database classifies -L-arabinofuranosidases in Glycoside Hydrolase (GH) families GH2, GH3, GH43, GH51, GH54 and GH62. Only GH62 contains exclusively -L-arabinofuranosidases and these are of fungal and bacterial origin. Twenty-two GH62 enzymes out of 223 entries in the CAZy database have been characterized and very recently new knowledge was acquired with regard to crystal structures, substrate specificities, and phylogenetics, which overall provides novel insights into structure/function relationships of GH62. Overall GH62 -L-arabinofuranosidases are believed to play important roles in nature by acting in synergy with several cell wall degrading enzymes and members of GH62 represent promising candidates for biotechnological improvements of biofuel production and in various biorefinery applications.3

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Section: Biorefineriesmentioning

confidence: 99%

GH62 arabinofuranosidases: Structure, function and applications

Wilkens

Andersen

Dumon

et al. 2017

Biotechnology Advances

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“…Current strategies for bioethanol production from lignocellulosic feedstocks require three major operational steps: physicochemical pretreatment, enzymatic saccharification, and fermentation ( Fig. 1) (6,12). Pretreatment and enzymatic hydrolysis represent substantial cost and it is estimated that the use of cellulolytic microbes for consolidated bioprocessing and eliminating pretreatment would reduce bioprocessing costs by 40% (2).…”

mentioning

confidence: 99%

Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii

Chung

Cha

Guss

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

196

171

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Ethanol is the most widely used renewable transportation biofuel in the United States, with the production of 13.3 billion gallons in 2012 [John UM (2013) Contribution of the Ethanol Industry to the Economy of the United States]. Despite considerable effort to produce fuels from lignocellulosic biomass, chemical pretreatment and the addition of saccharolytic enzymes before microbial bioconversion remain economic barriers to industrial deployment [Lynd LR, et al. (2008) Nat Biotechnol 26(2):169-172]. We began with the thermophilic, anaerobic, cellulolytic bacterium Caldicellulosiruptor bescii, which efficiently uses unpretreated biomass, and engineered it to produce ethanol. Here we report the direct conversion of switchgrass, a nonfood, renewable feedstock, to ethanol without conventional pretreatment of the biomass. This process was accomplished by deletion of lactate dehydrogenase and heterologous expression of a Clostridium thermocellum bifunctional acetaldehyde/alcohol dehydrogenase. Whereas wild-type C. bescii lacks the ability to make ethanol, 70% of the fermentation products in the engineered strain were ethanol [12.8 mM ethanol directly from 2% (wt/vol) switchgrass, a real-world substrate] with decreased production of acetate by 38% compared with wild-type. Direct conversion of biomass to ethanol represents a new paradigm for consolidated bioprocessing, offering the potential for carbon neutral, cost-effective, sustainable fuel production. metabolic engineering | bioenergy and thermophiles

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“…This results in the cellulose becoming obstructed during enzymatic hydrolysis, higher lignin content in the lignocellulosic matrix leading to stronger hydrolysis hindrance. Different approaches to breaking down this matrix are known (chemical, physicochemical, or mechanical treatment 26,27 ), but using catalytic oxidation for this purpose has not been described in literature. as can be seen in Figure 2a.…”

Section: 18mentioning

confidence: 99%

Processing Pine Wood into Vanillin and Glucose by Sequential Catalytic Oxidation and Enzymatic Hydrolysis

Tarabanko

Kaygorodov

Скиба

et al. 2016

Journal of Wood Chemistry and Technology

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Pine wood was processed into vanillin (up to 18 wt. % based on the lignin) and cellulose (typically 84-93 % of the initial amount in the wood) by one-stage catalytic oxidation, followed by enzymatic hydrolysis of the resulting cellulose into glucose (reducing sugar yield up to 70 % based on the cellulose). Correlation between the cellulose conversion in hydrolysis and the lignin 2 content in the post-oxidation lignocellulosic material was established, conforming to the general trend for the products of various delignification methods. The obtained results demonstrate the practical possibility of efficient two-step processing of wood into vanillin and glucose.

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Plant cell walls to ethanol

Cited by 167 publications

References 191 publications

GH62 arabinofuranosidases: Structure, function and applications

GH62 arabinofuranosidases: Structure, function and applications

Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii

Processing Pine Wood into Vanillin and Glucose by Sequential Catalytic Oxidation and Enzymatic Hydrolysis

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