Plant Cell Strains in Fundamental Research and Applications

Opatrný, Z.; Nick, Peter; Petrášek, Jan

doi:10.1007/978-3-642-41787-0_15

Cited by 10 publications

(7 citation statements)

References 83 publications

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“…Plant cell cultures are not only a promising platform to produce secondary metabolites but can also serve as a system of reduced complexity to address cellular mechanisms. Although plant cells in culture are often considered as “dedifferentiated”, they often preserve certain features from their source tissue (Opatrný et al 2014 ). To fully unfold the potential of cell-culture systems, it is important to lend more importance to this innate differentiation between cells.…”

Section: Discussionmentioning

confidence: 99%

Switching cell fate by the actin–auxin oscillator in Taxus: cellular aspects of plant cell fermentation

et al. 2022

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Key message Paclitaxel synthesis in Taxus cells correlates with a cell-fate switch that leads to vacuoles of a glossy appearance and vermiform mitochondria. This switch depends on actin and apoplastic respiratory burst. Abstract Plant cell fermentation, the production of valuable products in plant cell culture, has great potential as sustainable alternative to the exploitation of natural resources for compounds of pharmaceutical interest. However, the success of this approach has remained limited, because the cellular aspects of metabolic competence are mostly unknown. The production of the anti-cancer alkaloid Paclitaxel has been, so far, the most successful case for this approach. In the current work, we map cellular aspects of alkaloid synthesis in cells of Taxus chinensis using a combination of live-cell imaging, quantitative physiology, and metabolite analysis. We show evidence that metabolic potency correlates with a differentiation event giving rise to cells with large vacuoles with a tonoplast that is of a glossy appearance, agglomerations of lipophilic compounds, and multivesicular bodies that fuse with the plasma membrane. Cellular features of these glossy cells are bundled actin, more numerous peroxisomes, and vermiform mitochondria. The incidence of glossy cells can be increased by aluminium ions, and this increase is significantly reduced by the actin inhibitor Latrunculin B, and by diphenylene iodonium, a specific inhibitor of the NADPH oxidase Respiratory burst oxidase Homologue (RboH). It is also reduced by the artificial auxin Picloram. This cellular fingerprint matches the implications of a model, where the differentiation into the glossy cell type is regulated by the actin–auxin oscillator that in plant cells acts as dynamic switch between growth and defence.

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Section: Discussionmentioning

confidence: 99%

Switching cell fate by the actin–auxin oscillator in Taxus: cellular aspects of plant cell fermentation

et al. 2022

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“…Thus, plant cell fermentation represents an encouraging alternative resource for these medicinal compounds. The common view that cells in suspension are just "de-differentiated biomass" is certainly wrong -a closer look usually reveals that they have preserved certain features from their source tissue (Opatrný et al 2014). In the current work, we have shown that two strains derived from Catharanthus seeds not only differ in morphology, but also with respect to their metabolic competence.…”

Section: Discussionmentioning

confidence: 99%

“…In the leaf of Catharanthus, TIA metabolism is partitioned to different cell types (Ziegler and Facchini 2008), which, upon elicitation, express different branches of the metabolic network. While suspension cells are often interpreted as "de-differentiated", they usually maintain certain features of their source tissues and, therefore, often differ in their response to exogenous factors such as phytohormones or elicitors (Opatrný et al 2014). For such cellular differences in the response to external factors, the term "competence" has been coined (Mohr 1972).…”

Section: Introductionmentioning

confidence: 99%

Cell Type Matters: Competence For Alkaloid Metabolism Differs In Two Seed-Derived Cell Strains of Catharanthus Roseus.

Raorane

Manz

Hildebrandt

et al. 2021

Preprint

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Since the discovery of the anticancer drugs vinblastine and vincristine, Catharanthus roseus has been intensively studied for biosynthesis of several terpene indole alkaloids (TIAs). Due to their low abundance in plant tissues at a simultaneously high demand, modes of production alternative to conventional extraction are mandatory. Plant cell fermentation might become one of these alternatives, yet decades of research have shown limited success to certain product classes, leading to the question, how to preserve the intrinsic ability to produce TIAs (metabolic competence) in cell culture? We used the strategy to use the developmental potency of mature embryos to generate such strains. Two cell strains (C1and C4) from seeds of Catharanthus roseus were found not only to differ morphologically, but also in their metabolic competence. This differential competence became manifest under phytohormone elicitation, but also upon feeding with alkaloid pathway precursors. The more active strain C4 formed larger cell aggregates and was endowed with longer mitochondria. These cellular features were accompanied by higher alkaloid accumulation in response to methyl jasmonate (MeJA) elicitation. The levels of catharanthine could be increased significantly, while the concurrent vindoline branch of the pathway was blocked, such that no bisindole alkaloids were detectable. By feeding vindoline to MeJA elicited C4 cells, vincristine became detectable; however, only to marginal amounts. In conclusion, these results show that cultured cells are not just “de-differentiated”, but can differ in metabolic competence. In addition to elicitation, and precursor feeding, the cellular properties of the “biomatter” are highly relevant for the success of plant cell fermentation.

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“…Alternative experimental approaches, such as the use of plant cell strains, might still have an additional value to shed light into different puzzling phenomena that cannot be disentangled in planta. Indeed, single cell approaches, through the use of plant cell strains, have already brought very valuable knowledge into fundamental questions, including how auxin is transported in plants, and in how cell shape and cell polarity are generated [99].…”

Section: Discussionmentioning

confidence: 99%

Single-Cell Approaches for Understanding Morphogenesis Using Computational Morphodynamics

Formosa-Jordan

Teles

Jönsson

2018

Mathematical Modelling in Plant Biology

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In multicellular organisms cells grow, divide and adopt different fates, resulting in tissues and organs with specific functions. In recent years, a number of studies have brought quantitative knowledge about how these processes are orchestrated, shedding new light on cells as active and central players in morphogenesis. We explore recent advances in understanding plant morphogenesis from a quantitative perspective, defining the research field of Computational Morphodynamics. The focus is on studies combining theoretical and experimental approaches integrating hypotheses of how molecular and mechanical regulation at the cellular level lead to tissue behaviour. Finally, we discuss some of the main challenges for future work.

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Plant Cell Strains in Fundamental Research and Applications

Cited by 10 publications

References 83 publications

Switching cell fate by the actin–auxin oscillator in Taxus: cellular aspects of plant cell fermentation

Switching cell fate by the actin–auxin oscillator in Taxus: cellular aspects of plant cell fermentation

Cell Type Matters: Competence For Alkaloid Metabolism Differs In Two Seed-Derived Cell Strains of Catharanthus Roseus.

Single-Cell Approaches for Understanding Morphogenesis Using Computational Morphodynamics

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