Plant Cell Bioprocesses

Marchev, Andrey S.; Georgiev, Milen I.

doi:10.1016/b978-0-444-63663-8.00004-5

Cited by 3 publications

(2 citation statements)

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“…A high cell density usually makes it difficult to mix a cell culture and leads to a decrease in oxygen mass transfer [45], i.e., to a certain degree of hypoxia. This should lead to the accumulation of ethylene, which is good seen, for example, when plants are flooded [46].…”

Section: Tag Content and Fatty Acid Composition Of Total Lipids Durin...mentioning

confidence: 99%

Light and Methyl Jasmonate Impact in the Biosynthesis of Anthocyanins and VLCFAs in the <em>Euonymus maximowiczianus</em> Aril-Derived Long-Term Suspension-Cultured Cells

Nosov,

Fomenkov,

Sidorov

et al. 2023

Preprint

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The genus Euonymus (L.) consists of shrubs or woody plants, distributed mainly in the Northern Hemisphere. To date, from Euonymus spp. several hundreds of different secondary metabolites have been isolated and identified. In addition, fatty oil was found in the fruits of some species of these plants, which accumulates not only in the seeds, but also in the arils. This study presents the research of unique long-term (ten-year-old) suspension cell cultures of Euonymus maximoviczianus Prokh. which were obtained from arils of unripe capsules. Suspension cells retain the ability to form oil droplets containing neutral lipids, both in the dark and in the light, the cells are able to synthesize very-long-chain fatty acids (VLCFAs), and can synthesize delphinidin-3-O-hexoside, cyanidin-3-O-hexoside, and peonidin-3-O-hexoside. Here, we research of the fatty acid, VLCFA, and anthocyanin biosynthesis dynamics in the subcultivation, as well as the influence of methyl jasmonate (MeJa) on these processes in the dark- and under-light-grown cell cultures. In the darkness, the formation of VLCFAs is more intense, and the biosynthesis of anthocyanins is significantly activated in the light. MeJA substantially enhances the biosynthesis of anthocyanins in the light and, surprisingly, the formation of VLCFAs in the darknss. In connection with the commonality of the cytosolic pool of malonyl-CoA, which is necessary both for the biosynthesis of VLCFAs and dihydroflavonols (and, ultimately, anthocyanins), the competition of these biosynthetic pathways is discussed.

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Section: Tag Content and Fatty Acid Composition Of Total Lipids Durin...mentioning

confidence: 99%

Light and Methyl Jasmonate Impact in the Biosynthesis of Anthocyanins and VLCFAs in the <em>Euonymus maximowiczianus</em> Aril-Derived Long-Term Suspension-Cultured Cells

Nosov,

Fomenkov,

Sidorov

et al. 2023

Preprint

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“…Further, proliferation in the populations of Em-D and Em-L cells subside; the cells enter the stationary stage of growth, although the processes of water uptake and increase in FW continue (Figure 2a). By the end of subcultivation, a high cell biomass density is reached, about 300 g/L (Figure 2a), which usually makes it difficult to mix the cell culture and leads to a decrease in oxygen mass transfer [37], i.e., to a certain degree of hypoxia and, which is good seen when plants are flooded, to the accumulation of ethylene [38], in addition, by the end of subcultivation the nutrient medium is depleted of sucrose, nitrogen, phosphorus and other nutrients; all these factors activate cell senescence that terminates with programmed cell death [39,40]. In any way, after the completion of active divisions, cells enter a stage of proliferative quiescence, and not a metabolic rest, which can manifest itself in a change in the activity of some biosynthetic or catabolic pathways.…”

Section: Tag Content and Fatty Acid Composition Of Total Lipids Durin...mentioning

confidence: 99%

Light and Methyl Jasmonate Impacts in the Biosynthesis of Anthocyanins and VLCFAs in the Euonymus maximowiczianus Aril-Derived Long-Term Cell Cultures

Nosov,

Fomenkov,

Sidorov

et al. 2023

Preprint

View full text Add to dashboard Cite

The genus Euonymus (L.) consists of shrubs or woody plants, distributed mainly in the Northern Hemisphere. To date, from Euonymus spp. several hundred of different secondary metabolites have been isolated and identified. In addition, fatty oil was found in the fruits of some species of these plants, which accumulates not only in the seeds, but also in the arils. This study presents the research of unique long-term (ten-year-old) suspension cell cultures of Euonymus maximoviczianus Prokh. which were obtained from arils of unripe capsules. Suspension cells retain the ability to form oil droplets containing neutral lipids, both in the dark and in the light, the cells are able to synthesize very-long-chain fatty acids (VLCFAs), and can synthesize delphinidin-3-O-hexoside, cyanidin-3-O-hexoside, and peonidin-3-O-hexoside. Here, we research of the in subcultivation dynamics FA, VLCFA, and anthocyanin biosynthesis, as well as the influence of methyl jasmonate (MeJa) and light on these processes. In the darkness, the formation of VLCFAs is more intense, and the biosynthesis of anthocyanins is significantly activated in the light. MeJA substantially enhances the biosynthesis of anthocyanins in the light and, surprisingly, the formation of VLCFAs in the darknss. In connection with the commonality of the cytosolic pool of malonyl-CoA, which is necessary both for the biosynthesis of VLCFAs and dihydroflavonols (and, ultimately, anthocyanins), the competition and partnership of these biosynthetic pathways are discussed.

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