Plant Callus: Mechanisms of Induction and Repression

Ikeuchi, Momoko; Sugimoto, Keiko; Iwase, Akira

doi:10.1105/tpc.113.116053

Cited by 602 publications

(506 citation statements)

References 125 publications

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“…1), although in practice it can be difficult to distinguish between the two, and both often occur on the same explant. Indirect SE is the most common pathway, and starts with the formation of a callus, a seemingly unorganized mass of initially vacuolated cells that show different degrees of compactness (Ikeuchi, Sugimoto, & Iwase, 2013). Due to its initially amorphous structure, callus was (and often still is) referred to as “undifferentiated” or “dedifferentiated,” but these terms are rather ambiguous in the absence of more precise molecular information.…”

Section: Definitions and Conceptsmentioning

confidence: 99%

“…Overexpression of another member of the AP2/ERF TF family, WOUND INDUCED DEDIFFERENTIATION 1 ( WIND1 ) or RAP2.4 , also induces SE (Ikeuchi et al., 2013). WIND1 and its close homologs WIND2−4 are induced by wounding and stimulate callus proliferation after tissue damage (Iwase et al., 2011).…”

Section: A Network Of Arabidopsis Transcription Factors Controls Somamentioning

confidence: 99%

“…WIND1 and its close homologs WIND2−4 are induced by wounding and stimulate callus proliferation after tissue damage (Iwase et al., 2011). Ectopic WIND1 expression is sufficient to promote callus formation from shoots, hypocotyls, and roots (Iwase et al., 2011), which can then give rise to shoots, roots, or somatic embryos (Ikeuchi et al., 2013). …”

Section: A Network Of Arabidopsis Transcription Factors Controls Somamentioning

confidence: 99%

“…The co‐occurrence of callus, adventitious organs and somatic embryos in seedlings that overexpress regeneration‐promoting TFs ( LEC2 , Stone et al., 2001; WIND1 , Ikeuchi et al., 2013; BBM , Boutilier et al., 2002, Horstman et al., 2017; WUS , Zuo et al., 2002, Gallois et al., 2004) parallels observations in 2,4‐D SE cultures and lends further support to the idea that SE and organogenesis are very closely related processes that represent different outputs of a developmental continuum.…”

Section: A Network Of Arabidopsis Transcription Factors Controls Somamentioning

confidence: 99%

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A transcriptional view on somatic embryogenesis

2017

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Somatic embryogenesis is a form of induced plant cell totipotency where embryos develop from somatic or vegetative cells in the absence of fertilization. Somatic embryogenesis can be induced in vitro by exposing explants to stress or growth regulator treatments. Molecular genetics studies have also shown that ectopic expression of specific embryo‐ and meristem‐expressed transcription factors or loss of certain chromatin‐modifying proteins induces spontaneous somatic embryogenesis. We begin this review with a general description of the major developmental events that define plant somatic embryogenesis and then focus on the transcriptional regulation of this process in the model plant Arabidopsis thaliana (arabidopsis). We describe the different somatic embryogenesis systems developed for arabidopsis and discuss the roles of transcription factors and chromatin modifications in this process. We describe how these somatic embryogenesis factors are interconnected and how their pathways converge at the level of hormones. Furthermore, the similarities between the developmental pathways in hormone‐ and transcription‐factor‐induced tissue culture systems are reviewed in the light of our recent findings on the somatic embryo‐inducing transcription factor BABY BOOM.

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Section: Definitions and Conceptsmentioning

confidence: 99%

Section: A Network Of Arabidopsis Transcription Factors Controls Somamentioning

confidence: 99%

Section: A Network Of Arabidopsis Transcription Factors Controls Somamentioning

confidence: 99%

Section: A Network Of Arabidopsis Transcription Factors Controls Somamentioning

confidence: 99%

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A transcriptional view on somatic embryogenesis

2017

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“…Recent studies have shown that lateral root primordium initiation is required for callus formation, as mutants which fail to initiate lateral root primordia are unable to make any callus [9,11,17]. To understand the mechanisms controlling the intermediate steps leading to shoot regeneration, mutants that are blocked at different developmental phases of shoot regeneration need to be examined.…”

Section: Plt3 Plt5 and Plt7 Display Dynamic Expression Patterns Durimentioning

confidence: 99%

PLETHORA Genes Control Regeneration by a Two-Step Mechanism

et al. 2015

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SummaryBackground-Regeneration, a remarkable example of developmental plasticity displayed by both plants and animals, involves successive developmental events driven in response to environmental cues. Despite decades of study on the ability of the plant tissues to regenerate complete fertile shoot system after inductive cues, the mechanisms by which cells acquire pluripotency and subsequently regenerate complete organs remain unknown.

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Thirty Years of Genome Engineering in Rice: From Gene Addition to Gene Editing

Meynard

Vernet

Meunier

et al. 2020

Annual Plant Reviews Online

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Rice, the staple food for more than half of humankind and the model monocot crop, was the first cereal in which efficient gene transfer procedures were implemented: 30 years ago, the first transgenic rice plant was regenerated following direct gene transfer to cell suspension‐derived protoplasts. Shortly thereafter, transgenic plants were regenerated from zygotic embryo‐derived cells following their subjection to micro‐projectile acceleration and Agrobacterium ‐mediated transfection treatments. The high efficiency of transfer deoxyribonucleic acid (T‐DNA) integration in seed embryo‐derived cells of rice has allowed the transfer of genes of agronomical relevance and the generation of large collections of insertion lines and has provided a key contribution to deciphering the function of more than 2000 rice genes. The high efficiency of T‐DNA integration in seed embryo‐derived cells of rice also permitted the first implementation of gene targeting and knock‐in (KI) events, relying on the albeit very low natural frequency of homology‐directed repair (HDR) in the rice genome. In the late 2000s, the advent of site‐directed nucleases (SDNs) that induce either single or double‐strand breaks at a high frequency and their rapid application to rice permitted routine targeted mutagenesis, which can be multiplexed to simultaneously alter several targets or create deletions, and base and gene editing (e.g. correction of amino acids). Currently, the challenge remains to attain a high frequency of KI and replacement of long stretches of DNA for protein domain or coding sequence swapping. We present herein a historical perspective of the advances that have been readily implemented to determine the function of rice genes and to manipulate traits of agronomical relevance. Two main bottlenecks remain to be alleviated in rice genomic engineering: the low frequency of HDR and the genotype dependence of gene transfer efficiency. Alleviation of these bottlenecks is needed to reach the potential of intra‐ and interspecific gene replacement and SDN‐mediated multiplex editing of alleles in elite materials, which will assist in the breeding and deployment of rice cultivars embedded in sustainable and climate‐smart agricultural practices.

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Plant Callus: Mechanisms of Induction and Repression

Cited by 602 publications

References 125 publications

A transcriptional view on somatic embryogenesis

A transcriptional view on somatic embryogenesis

PLETHORA Genes Control Regeneration by a Two-Step Mechanism

Thirty Years of Genome Engineering in Rice: From Gene Addition to Gene Editing

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