Plant-based Expression of SARS-CoV-2 antigens for use in an Oral Vaccine

Power, Monique; Azad, Taha; Bell, John C.; MacLean, Allyson M.

doi:10.26502/jfsnr.2642-110000101

Cited by 2 publications

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“…Cultivated lettuce ( Lactuca sativa ), domesticated from prickly lettuce ( L. serriola ), is one of the most important green leafy vegetables worldwide (Zhang et al ., 2017). Lettuce is also a model species for hydroponics, and an ideal plant to be engineered to produce oral vaccines or valuable pharmaceuticals (Daniell et al ., 2001; Kanamoto et al ., 2006; Lal et al ., 2007; Power et al ., 2021). Both cultivated and wild lettuce have the polymorphism of lobed and non‐lobed leaves.…”

Section: Introductionmentioning

confidence: 99%

The upregulated LsKN1 gene transforms pinnately to palmately lobed leaves through auxin, gibberellin, and leaf dorsiventrality pathways in lettuce

Wang

Lavelle

et al. 2022

Plant Biotechnology Journal

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Leaf shape represents a vital agronomic trait for leafy vegetables such as lettuce. Some lettuce cultivars produce lobed leaves, varying from pinnately to palmately lobed, but the genetic mechanisms remain unclear. In this study, we cloned one major quantitative trait locus (QTL) controlling palmately lobed leaves. The candidate gene, LsKN1, encodes a homeobox transcription factor, and has been shown previously to be critical for the development of leafy heads in lettuce. The LsKN1 allele that is upregulated by the insertion of a transposon promotes the development of palmately lobed leaves. We demonstrated that LsKN1 upregulated LsCUC2 and LsCUC3 through different mechanisms, and their upregulation was critical for the development of palmately lobed leaves. LsKN1 binds the promoter of LsPID to promote auxin biosynthesis, which positively contributes to the development of palmately lobed leaves. In contrast, LsKN1 suppresses GA biosynthesis to promote palmately lobed leaves. LsKN1 also binds to the promoter of LsAS1, a dorsiventrality gene, to downregulate its expression. Overexpression of the LsAS1 gene compromised the effects of the LsKN1 gene changing palmately to pinnately lobed leaves. Our study illustrated that the upregulated LsKN1 gene led to palmately lobed leaves in lettuce by integrating several downstream pathways, including auxin, gibberellin, and leaf dorsiventrality pathways.

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Section: Introductionmentioning

confidence: 99%

The upregulated LsKN1 gene transforms pinnately to palmately lobed leaves through auxin, gibberellin, and leaf dorsiventrality pathways in lettuce

Wang

Lavelle

et al. 2022

Plant Biotechnology Journal

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Lskipk Lsatpase double mutants are necessary and sufficient for the compact plant architecture of butterhead lettuce

Xie,

Luo,

et al. 2023

Horticulture Research

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Lettuce, an important leafy vegetable crop worldwide, has rich variations in plant architecture. Butterhead lettuce, a popular horticulture type, has a unique plant architecture with a loose leafy head. The genetic and molecular mechanisms for such a compact plant architecture remain unclear. In this study, we constructed a segregating population through crossing a butterhead cultivar and a stem lettuce cultivar. Genetic analysis identified the LsKIPK gene, which encodes a kinase, as the candidate gene controlling butterhead plant architecture. The Lskipk gene in the butterhead parent had a nonsense mutation, leading to a partial predicted protein. CRISPR/Cas9 and complementation tests verified its functions on plant architecture. We showed that the loss-of-function of LsKIPK is necessary but not sufficient for the butterhead plant architecture. To identify additional gene required for butterhead lettuce, we crossed a butterhead cultivar and a crisphead cultivar, both with the mutated Lskipk gene. Genetic mapping identified a new gene encoding a ATPase contributing to butterhead plant architecture. Knockout and complementation tests showed that the loss-of-function of LsATPase is also required for the development of butterhead plant architecture. Lskipk Lsatpase double mutants could reduce leaf size and leaf angle, leading to butterhead plant architecture. Expression and cytology analysis indicated that the loss-of-function of LsKIPK and LsATPase contributed to butterhead plant architecture by regulating cell wall development, a regulatory mechanism different from that for crisphead. This study provides new gene resources and theory for the breeding of crop ideotype.

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Plant-based Expression of SARS-CoV-2 antigens for use in an Oral Vaccine

Cited by 2 publications

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The upregulated LsKN1 gene transforms pinnately to palmately lobed leaves through auxin, gibberellin, and leaf dorsiventrality pathways in lettuce

The upregulated LsKN1 gene transforms pinnately to palmately lobed leaves through auxin, gibberellin, and leaf dorsiventrality pathways in lettuce

Lskipk Lsatpase double mutants are necessary and sufficient for the compact plant architecture of butterhead lettuce

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