Plant-bacterium interactions analyzed by proteomics

Abstract

The evolution of the plant immune response has resulted in a highly effective defense system that is able to resist potential attack by microbial pathogens. The primary immune response is referred to as pathogen associated molecular pattern (PAMP) triggered immunity and has evolved to recognize common features of microbial pathogens. In response to the delivery of pathogen effector proteins, plants acquired R proteins to fight against pathogen attack. R-dependent defense response is important in understanding the biochemical and cellular mechanisms and underlying these interactions will enable molecular and transgenic approaches for crops with increased biotic resistance. Proteomic analyses are particularly useful for understanding the mechanisms of host plant against the pathogen attack. Recent advances in the field of proteome analyses have initiated a new research area, i.e., the analysis of more complex microbial communities and their interaction with plant. Such areas hold great potential to elucidate, not only the interactions between bacteria and their host plants, but also of bacteria-bacteria interactions between different bacterial taxa, symbiotic, pathogenic bacteria, and commensal bacteria. During biotic stress, plant hormonal signaling pathways prioritizes defense over other cellular functions. Some plant pathogens take advantage of hormone dependent regulatory system by mimicking hormones that interfere with host immune responses to promote virulence (vir). In this review, it is discussed the cross talk that plays important role in response to pathogens attack with different infection strategies using proteomic approaches.

Systems-level Proteomics of Two Ubiquitous Leaf Commensals Reveals Complementary Adaptive Traits for Phyllosphere Colonization

“…Here we extended earlier proteomic analyses of microbial plant community members (10,56,57) by applying the massively parallel targeted proteomic technique SWATH MS to reliably quantify the proteomic changes of two ubiquitous plant commensals upon colonization of A. thaliana leaves. Methylobacterium and Sphingomonas are two genera commonly inhabiting the phyllosphere of different host plants and previous studies suggested distinct metabolic capabilities to be important drivers for niche separation (12).…”

Sphingobacterium sp. BHU-AV3 Induces Salt Tolerance in Tomato by Enhancing Antioxidant Activities and Energy Metabolism

“…Only a few reports are available on combinational effects of beneficial microbes and salt stress through protein study, and these show that most of the expressed proteins in plants under salinity stress are related to transcription and translation factors, photosynthesis, lignin biosynthesis, and antioxidative and defense proteins (Cheng et al, 2012;Vaishnav et al, 2015;Singh et al, 2017). However, plant growth response to microbial application may vary with the experimental conditions, microbial diversity, and plant functional groups (Afroz et al, 2013).…”

Bradyrhizobium diazoefficiens USDA 110-Glycine max interactome provides candidate proteins associated with symbiosis

“…Generally, the proteins in the symbiosis process function as a complex network, which combines complex chemical, physical and biological interactions between rhizobial bacteria and their host plants [8]. To better elucidate the complex microbial communities and investigate the mechanism of nitrogen-fixing symbiosis, it is necessary to construct the protein interactions between rhizobium and their host legume plants [9].…”