Plant antimicrobial peptides: a comprehensive review of their classification, production, mode of action, functions, applications, and challenges

Zou, Fanglei; Tan, Chunming; Shinali, Tharushi S; Zhang, Bo; Zhang, Lili; Han, Zixin; Shang, Nan

doi:10.1039/d3fo01119d

Cited by 14 publications

(6 citation statements)

References 167 publications

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“…2). Реализация защитной функции растений с участием АМП определяется преимущественным образом их прямым (контактным) действием на фитопатогены, которое заключается в воздействии на цитоплазматическую мембрану с последующим ее разрушением и гибелью клетки (тионины, дефензины, липид-переносящие белки, циклотиды) (Thevissen et al, 1999;Taveira et al, 2018;Zou et al, 2023). В ряде случаев АМП связываются с углеводными компонентами клеточной стенки грибов-микромицетов (хитином, альфа-1,4/бета-1,4-глюканами), что в дальнейшем приводит к аномальному развитиюмицелия, нарушению его дифференциации и проявлению так называемой морфогенной активности (дефензины, гевеино-подобные пептиды, альфа-харпинины) (Broekaert et al, 1995;Rogozhin et al, 2015.…”

Section: перспективы применения антимикробных пептидов растений в рас...unclassified

“…Стоит отметить, что на фоне использования химических средств защиты растений, биологические методы занимают крайне небольшую долю и их использование имеет целый ряд ограничений, в связи с чем многие производители сельскохозяйственной продукции вынуждены либо полностью отказываться от них, либо объединять их с «химией» в рамках одной системы защитных мероприятий. Антимикробные вещества (в том числе и пептиды) представляют интерес как для медицины, так и для сельского хозяйства (Marcos et al, 2008;Das et al, 2019;Zou et al, 2023).…”

Section: Introductionunclassified

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The potential of plant antimicrobial peptides for crop protection against diseases

Barashkova,

Rogozhin*

2023

1727-1320

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Antimicrobial peptides (AMPs) are the most important components of plant innate immunity to environmental stress factors and one of the most ancient tools of the defense system. Most of them are synthesized as factors of constitutive plant immunity, but there are also inducible forms belonging to “pathogenesis-related proteins” (PR-proteins from classes 12, 13 and 14). This review provides characteristics of the primary and three-dimensional structures of the main families of plant AMPs. The relationship between the types of spatial arrangement of the polypeptide chain is drawn. The functional analysis of plant AMPs is presented by data on the spectrum and quantitative level of activities against a number of economically significant fungal and bacterial phytopathogens, and related biological effects are indicated. Additionally, current information is provided on the molecular mechanisms of the antimicrobial action of plant AMPs based on defensins as the most studied structural group. In conclusion, aspects of the modes of action for plant AMPs on microorganisms are considered, on the basis of which a variant of the functional classification of these molecules is proposed. Based on these data, the prospects for their use as the basis of biopesticides for plant protection against pathogens were assessed.

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Section: перспективы применения антимикробных пептидов растений в рас...unclassified

Section: Introductionunclassified

The potential of plant antimicrobial peptides for crop protection against diseases

Barashkova,

Rogozhin*

2023

1727-1320

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“…Plant additives contain bioactive substances such as alkaloids, saponins, volatile oils, tannins, and polysaccharides, which have various functions such as sterilization, growth promotion, and oxidation resistance. They are considered one of the natural feed additives as substitutes for feed antibiotics (1)(2)(3)(4). In recent years, research on the nutritional regulation and production application of Chinese herbal medicine in livestock and poultry has received widespread attention (5,6).…”

Section: Introductionmentioning

confidence: 99%

Multi-omics analysis on the mechanism of the effect of Isatis leaf on the growth performance of fattening sheep

Cao,

Yi,

Zhou

et al. 2024

Front. Vet. Sci.

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IntroductionThis study evaluated the effects of Isatis Leaf (ISL) on the growth performance, gastrointestinal tissue morphology, rumen and intestinal microbiota, rumen, serum and urine metabolites, and rumen epithelial tissue transcriptome of fattening sheep.MethodsTwelve 3.5-month-old healthy fattening sheep were randomly divided into two groups, each with 6 replicates, and fed with basal diet (CON) and basal diet supplemented with 80 g/kg ISL for 2.5 months. Gastrointestinal tract was collected for histological analysis, rumen fluid and feces were subjected to metagenomic analysis, rumen fluid, serum, and urine for metabolomics analysis, and rumen epithelial tissue for transcriptomics analysis.ResultsThe results showed that in the ISL group, the average daily gain and average daily feed intake of fattening sheep were significantly lower than those of the CON group (P < 0.05), and the rumen ammonia nitrogen level was significantly higher than that of the CON group (P < 0.01). The thickness of the reticulum and abomasum muscle layer was significantly increased (P < 0.05). At the genus level, the addition of ISL modified the composition of rumen and fecal microorganisms, and the relative abundance of Methanobrevibacter and Centipeda was significantly upregulated in rumen microorganisms, The relative abundance of Butyrivibrio, Saccharofermentans, Mogibacterium, and Pirellula was significantly downregulated (P < 0.05). In fecal microorganisms, the relative abundance of Papillibacter, Pseudoflavonifractor, Butyricicoccus, Anaerovorax, and Methanocorpusculum was significantly upregulated, while the relative abundance of Roseburia, Coprococcus, Clostridium XVIII, Butyrivibrio, Parasutterella, Macellibacteroides, and Porphyromonas was significantly downregulated (P < 0.05). There were 164, 107, and 77 different metabolites in the rumen, serum, and urine between the ISL and CON groups (P < 0.05). The differential metabolic pathways mainly included thiamine metabolism, niacin and nicotinamide metabolism, vitamin B6 metabolism, taurine and taurine metabolism, beta-Alanine metabolism and riboflavin metabolism. These metabolic pathways were mainly involved in the regulation of energy metabolism and immune function in fattening sheep. Transcriptome sequencing showed that differentially expressed genes were mainly enriched in cellular physiological processes, development, and immune regulation.ConclusionIn summary, the addition of ISL to the diet had the effect of increasing rumen ammonia nitrogen levels, regulating gastrointestinal microbiota, promoting body fat metabolism, and enhancing immunity in fattening sheep.

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“…In numerous instances, the peptide moves into the microbial cell to target internal structures. Hence, AMPs have the potential to impact a variety of processes as they typically do not have a single target, but multiple [ 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ]. It is documented that certain AMPs have an affinity for lipid II, DNA and RNA, chaperones and ribosomes, proteases, adenosine triphosphate (ATP), and ABC transporters [ 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ].…”

Section: Introductionmentioning

confidence: 99%

Redefining Peptide 14D: Substitutional Analysis for Accelerated TB Diagnosis and Enhanced Activity against Mycobacterium tuberculosis

Hilpert,

Munshi,

López-Pérez

et al. 2024

Microorganisms

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Tuberculosis (TB) caused by Mycobacterium tuberculosis remains a predominant cause of mortality, especially in low- and middle-income nations. Recently, antimicrobial peptides have been discovered that at low concentrations could stimulate the growth of M. tuberculosis (hormetic response). In this study, such a peptide was used to investigate the effects on the time to positivity (TTP). A systematic substitution analysis of peptide 14D was synthesized using Spot synthesis technology, resulting in 171 novel peptides. Our findings revealed a spectrum of interactions, with some peptides accelerating M. tuberculosis growth, potentially aiding in faster diagnostics, while others exhibited inhibitory effects. Notably, peptide NH2-wkivfiwrr-CONH2 significantly reduced the TTP by 25 h compared to the wild-type peptide 14D, highlighting its potential in improving TB diagnostics by culture. Several peptides demonstrated potent antimycobacterial activity, with a minimum inhibitory concentration (MIC) of 20 µg/mL against H37Rv and a multidrug-resistant M. tuberculosis strain. Additionally, for two peptides, a strongly diminished formation of cord-like structures was observed, which is indicative of reduced virulence and transmission potential. This study underscores the multifaceted roles of antimicrobial peptides in TB management, from enhancing diagnostic efficiency to offering therapeutic avenues against M. tuberculosis.

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Plant antimicrobial peptides: a comprehensive review of their classification, production, mode of action, functions, applications, and challenges

Abstract: The emergence of pathogens resistance to conventional antibiotics and the growing interest in developing alternative natural antimicrobial agents have prompted a search for plant-derived antimicrobial peptides (PAMPs) in recent years....

Cited by 14 publications

References 167 publications

The potential of plant antimicrobial peptides for crop protection against diseases

The potential of plant antimicrobial peptides for crop protection against diseases

Multi-omics analysis on the mechanism of the effect of Isatis leaf on the growth performance of fattening sheep

Redefining Peptide 14D: Substitutional Analysis for Accelerated TB Diagnosis and Enhanced Activity against Mycobacterium tuberculosis

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