Planomonospora algeriensis sp. nov., an actinobacterium isolated from a Saharan soil of Algeria

Abstract

A filamentous actinobacterium, designated strain PM3, was isolated from a Saharan soil sample collected from Béni-Abbès, Béchar (South-West Algeria). A polyphasic taxonomic study was carried out to establish the status of strain PM3. The isolate was found to have morphological and chemotaxonomical properties associated with members of the genus Planomonospora. The new isolated microorganism developed cylindrical sporangia arranged in double parallel rows on aerial mycelium, each one containing a motile single sporangiospore. The cell wall of the strain was found to contain meso-diaminopimelic acid. Whole-cell hydrolysates were found to contain madurose, glucose, mannose and ribose. The predominant menaquinone was identified as MK-9(H) (69.6%). The polar lipids detected were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, phosphatidylhydroxyethanolamine and glucosamine-containing lipids. The major fatty acids were found to be Cω9c (38.6%) and C (24.2%). Results of 16S rRNA gene sequence comparison revealed that strain PM3 shared a high degree of 16S rRNA gene sequence similarity with Planomonospora sphaerica DSM 44632 (99.3%), Planomonospora parontospora subsp. parontospora DSM 43177 (99.2%) and P. parontospora subsp. antibiotica DSM 43869 (99.0%). DNA-DNA hybridization values between strain PM3 and the type strains of the closely related species were between 58.4 and 70.1%. The combination of phylogenetic analysis, DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic data support the conclusion that strain PM3 represents a novel species of the genus Planomonospora, for which the name Planomonospora algeriensis sp. nov. is proposed. The type strain is PM3 (=DSM 46752 = CECT 9047).

Planomonospora: A Metabolomics Perspective on an Underexplored Actinobacteria Genus

“…The 72 strains yielded 35 unique 16S rRNA sequences, 31 of which had not been previously reported (see Figures S1 and S2). The resulting phylogenetic tree (see Figure ) was found to be in agreement with previous studies , and showed three phylogroups with a relevant number of representatives: Phylogroup C includes 13 strains (9 of them from Naicons collection) and 9 distinct 16S rRNA sequences; phylogroup A2 includes 12 strains (11 of them from Naicons collection) and 8 distinct 16S rRNA sequences. The most populated phylogroup S includes 46 strains (43 of them from Naicons collection) and 13 distinct 16S rRNA sequences.…”

Identification of antagonistic Streptomyces strains isolated from Algerian Saharan soils and their plant growth promoting properties

“…Recently, there has been a new interest in the resources of actinobacteria from Algerian arid zones, considering that high temperatures can induce metabolic adaptations which could result in the production of interesting metabolites (Djinni et al 2019). Effectively, many studies have contributed to the finding of new species of actinobacteria in poorly explored arid environments, notably through the isolation of rare genera: Mzabimyces algeriensis (Saker et al 2014), Actinopolyspora algeriensis (Meklat et al 2012), Planomonospora algeriensis (Chaabane-Chaouch et al 2017). As a result, from 2002 to 2019, one new genus and twenty-nine new species were obtained, mainly from palm groves and Algerian Saharan soils.…”