Planktonic ciliate community driven by environmental variables and cyanobacterial blooms: A 9-year study in two subtropical reservoirs

Al, Mamun Abdullah; Wang, Wenping; Jin, Lan; Chen, Huihuang; Xue, Yuanyuan; Majaneva, Markus; Xu, Henglong; Yang, Jun

doi:10.1016/j.scitotenv.2022.159866

Cited by 2 publications

(3 citation statements)

References 64 publications

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“…Chlorophyta, Dinoflagellata and Ochrophyta) were greater after relic DNA removal, whereas Cercozoa and Ciliophora exhibited the opposite trends. It is well‐known that ciliates or cercozoans can feed on bacteria, fungi and even some eukaryotes (Abdullah Al et al, 2023; Pierce & Turner, 1992). Phytoplankton communities are a crucial functional component in maintaining ecosystem structure and functional stability (Yang et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

Relic DNA obscures DNA‐based profiling of multiple microbial taxonomic groups in a river‐reservoir ecosystem

Xue

Chen

et al. 2023

Molecular Ecology

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Numerous studies have investigated the spatiotemporal variability in water microbial communities, yet the effects of relic DNA on microbial community profiles, especially microeukaryotes, remain far from fully understood. Here, total and active bacterial and microeukaryotic community compositions were characterized using propidium monoazide (PMA) treatment coupled with high‐throughput sequencing in a river‐reservoir ecosystem. Beta diversity analysis showed a significant difference in community composition between both the PMA untreated and treated bacteria and microeukaryotes; however, the differentiating effect was much stronger for microeukaryotes. Relic DNA only resulted in underestimation of the relative abundances of Bacteroidota and Nitrospirota, while other bacterial taxa exhibited no significant changes. As for microeukaryotes, the relative abundances of some phytoplankton (e.g. Chlorophyta, Dinoflagellata and Ochrophyta) and fungi were greater after relic DNA removal, whereas Cercozoa and Ciliophora showed the opposite trend. Moreover, relic DNA removal weakened the size and complexity of cross‐trophic microbial networks and significantly changed the relationships between environmental factors and microeukaryotic community composition. However, there was no significant difference in the rates of temporal community turnover between the PMA untreated and treated samples for either bacteria or microeukaryotes. Overall, our results imply that the presence of relic DNA in waters can give misleading information of the active microbial community composition, co‐occurrence networks and their relationships with environmental conditions. More studies of the abundance, decay rate and functioning of nonviable DNA in freshwater ecosystems are highly recommended in the future.

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Section: Discussionmentioning

confidence: 99%

Relic DNA obscures DNA‐based profiling of multiple microbial taxonomic groups in a river‐reservoir ecosystem

Xue

Chen

et al. 2023

Molecular Ecology

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“…Three-metre samples covered mostly the entire epilimnion and did not penetrate to metalimnion during the stratification of the Slapy reservoir. The same monitoring approach has persisted over the past decades, focusing on the surface or epilimnion layer despite variations in water bodies worldwide (recently, e.g., [37]). Notably, this method is not universal, particularly in water bodies with shallow epilimnion that do not align perfectly with the euphotic zone.…”

Section: Hydrodynamics Of the Surface Layer And A Legacy Of Defined P...mentioning

confidence: 99%

“…In many other cases, however, only ciliate samples from the surface layer or shallow epilimnion have been processed because such layers have been typically sampled for monitoring; moreover, due to the change in ciliate analysis to molecular methods, the possible number of processed samples has decreased (recently, [37]). However, adding ciliate analysis to zooplankton and/or phytoplanktonbased sampling programs would be inappropriate [2] Our study aims to analyse two decades of ciliate monitoring in the 3-m surface/epilimnion layer of the mesotrophic dimictic/monomictic reservoir Slapy (Czechia).…”

Section: Introductionmentioning

confidence: 99%

Changes in the Epilimnion Ciliate Assemblage Reflect the Reduction of the Nutrient Load of the Reservoir for Two Decades

Macek,

Vrba,

Hejzlar

et al. 2024

Preprint

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The awareness of ciliate importance in freshwater has changed dramatically in the last decades, reflecting methodological attempts. We analysed two decades (1994-2018) of data on the surface (0-3 m) ciliate assemblage in the Slapy reservoir (Vltava River, Czech Republic) during two different nutrient-load defined periods. We identified, quantified, and evaluated the biomass of ciliates in the quantitative protargol-stained preparations. We used water age, nutrients, bacteria, heterotrophic nanoflagellates and rhodomonads as the main explanatory background variables. The sampling median and interquartile range data analysis and a non-metric multidimensional scaling were applied. Ciliates were grouped according to their feeding behaviour. The picoplankton filtering species dominated the assemblages in an annual mean (halteriids and minute strobilidiids followed by peritrichs). Algae hunters Balanion planctonicum, urotrichs and nanoplankton filtering tintinnids were important before the spring phytoplankton peak when a maximum of ciliate biomass reflected mixotrophic nanoplankton filtering pelagostrombidiids. Only there, ciliate biomass tightly followed their quantified prey. Heterotrophic and mixotrophic Askenasia and Lagynophrya were typical raptorial/flagellate hunters; only Mesodinium spp. peaked during autumn. The oligotrophication increased the ciliate assemblage biomass in the surface layer during the stratification in concordance with the PEG model.

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Planktonic ciliate community driven by environmental variables and cyanobacterial blooms: A 9-year study in two subtropical reservoirs

Cited by 2 publications

References 64 publications

Relic DNA obscures DNA‐based profiling of multiple microbial taxonomic groups in a river‐reservoir ecosystem

Relic DNA obscures DNA‐based profiling of multiple microbial taxonomic groups in a river‐reservoir ecosystem

Changes in the Epilimnion Ciliate Assemblage Reflect the Reduction of the Nutrient Load of the Reservoir for Two Decades

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