Plane wave in vitro exposure of biological samples, geometries considerations

High power electromagnetic pulse applicators for evaluation of biological effects induced by electromagnetic radiation waves

“…This geometrical configuration allows to minimize the volume of the sample, to maximize the E field coupling factor and to maximize its homogeneity in the sample volume. 18 The coupled field into the sample at the center of the Petri dish ( Fig. 4a ) was numerically evaluated and its value was 48% of the incident radiated field.…”

“…4a ) was numerically evaluated and its value was 48% of the incident radiated field. 18 Consequently, we have limited the applied fields within the laboratory applicators to these values. For each condition tested, 400 GUVs were observed by phase contrast microscopy before and after exposure ( Fig.…”

“…The Petri dish was placed in front of antennas of the WB and UNB high power microwave systems; the H orientation was chosen; in such configuration the H field component was axially oriented and the E field component was coplanar with the agarose layer. 18 This orientation allows a coupling factor of 48% of the E field with the entire diameter dimension of the agarose layer and the homogeneity of the E field inside the sample volume, located at the center of the Petri dish is excellent due to similar dielectric properties of the agarose and the sample (DUT). 18 This method for preparing an object to be tested and for improving the uniformity and intensity of an electric field induced in said object illuminated by an incident electromagnetic wave is related to a patent (Pat., 20170184648, 2015, Publication date: 2017, Applicant: Commissariat à L'energie Atomique et aux Energies Alternatives, Inventors: R. Vezinet, A. Catrain, T. Chretiennot).…”

“… 18 This orientation allows a coupling factor of 48% of the E field with the entire diameter dimension of the agarose layer and the homogeneity of the E field inside the sample volume, located at the center of the Petri dish is excellent due to similar dielectric properties of the agarose and the sample (DUT). 18 This method for preparing an object to be tested and for improving the uniformity and intensity of an electric field induced in said object illuminated by an incident electromagnetic wave is related to a patent (Pat., 20170184648, 2015, Publication date: 2017, Applicant: Commissariat à L'energie Atomique et aux Energies Alternatives, Inventors: R. Vezinet, A. Catrain, T. Chretiennot). The thickness of the agarose layer has to be limited to 1 mm with the aim to avoid some dielectrics ringing modes in H orientation; see ref.…”

“… 18 This method for preparing an object to be tested and for improving the uniformity and intensity of an electric field induced in said object illuminated by an incident electromagnetic wave is related to a patent (Pat., 20170184648, 2015, Publication date: 2017, Applicant: Commissariat à L'energie Atomique et aux Energies Alternatives, Inventors: R. Vezinet, A. Catrain, T. Chretiennot). The thickness of the agarose layer has to be limited to 1 mm with the aim to avoid some dielectrics ringing modes in H orientation; see ref. 18 for details.…”