Plane of nutrition affects the phylogenetic diversity and relative abundance of transcriptionally active methanogens in the bovine rumen

McGovern, Emily; McCabe, Matthew S.; Cormican, Paul; Popova, Milka; Keogh, Kate; Kelly, A. K.; Kenny, David A.; Waters, Sinéad M.

doi:10.1038/s41598-017-13013-y

Cited by 16 publications

(12 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In rumen, Methanomicrobium converts H 2 and/or formate into CH 4 (Leahy et al, 2013). The abundance of Thermoplasmatales was also negatively correlated with Methanobrevibacter, which is consistent with previous results (Danielsson et al, 2017;McGovern et al, 2017).…”

Section: Archaeal Communitysupporting

confidence: 91%

Comparative analysis of the metabolically active microbial communities in the rumen of dromedary camels under different feeding systems using total rRNA sequencing

Rabee

Forster

Elekwachi

et al. 2020

PeerJ

View full text Add to dashboard Cite

Breakdown of plant biomass in rumen depends on interactions between bacteria, archaea, fungi, and protozoa; however, the majority of studies of the microbiome of ruminants, including the few studies of the rumen of camels, only studied one of these microbial groups. In this study, we applied total rRNA sequencing to identify active microbial communities in 22 solid and liquid rumen samples from 11 camels. These camels were reared at three stations that use different feeding systems: clover, hay and wheat straw (G1), fresh clover (G2), and wheat straw (G3). Bacteria dominated the libraries of sequence reads generated from all rumen samples, followed by protozoa, archaea, and fungi respectively. Firmicutes, Thermoplasmatales, Diplodinium, and Neocallimastix dominated bacterial, archaeal, protozoal and fungal communities, respectively in all samples. Libraries generated from camels reared at facility G2, where they were fed fresh clover, showed the highest alpha diversity. Principal co-ordinate analysis and linear discriminate analysis showed clusters associated with facility/feed and the relative abundance of microbes varied between liquid and solid fractions. This provides preliminary evidence that bacteria dominate the microbial communities of the camel rumen and these communities differ significantly between populations of domesticated camels.

show abstract

Section: Archaeal Communitysupporting

confidence: 91%

Comparative analysis of the metabolically active microbial communities in the rumen of dromedary camels under different feeding systems using total rRNA sequencing

Rabee

Forster

Elekwachi

et al. 2020

PeerJ

View full text Add to dashboard Cite

show abstract

“…All Euryarchaeota species found in the rabbit cecum belong to genus Methanobrevibacter that encompasses different hydrogenotrophic methane-producing species. Previous studies in humans [36] and cattle [37,38] found an overrepresentation of Methanobrevibacter species in individuals submitted to feed restriction and a negative correlation between the abundance of this bacterium and body mass index. A prevalence of Methanobrevibacter species could be a positive indicator of a healthy microbiota since restricted animals showed an overrepresentation of this genus.…”

Section: Feed Restriction Modify Euryarchaeota and Some Bacteria Relamentioning

confidence: 83%

Breeding farm, level of feeding and presence of antibiotics in the feed influence rabbit cecal microbiota

et al. 2020

View full text Add to dashboard Cite

Background The effect of the production environment and different management practices in rabbit cecal microbiota remains poorly understood. While previous studies have proved the impact of the age or the feed composition, research in the breeding farm and other animal management aspects, such as the presence of antibiotics in the feed or the level of feeding, is still needed. Characterization of microbial diversity and composition of growing rabbits raised under different conditions could help better understand the role these practices play in cecal microbial communities and how it may result in different animal performance. Results Four hundred twenty-five meat rabbits raised in two different facilities, fed under two feeding regimes (ad libitum or restricted) with feed supplemented or free of antibiotics, were selected for this study. A 16S rRNA gene-based assessment through the MiSeq Illumina sequencing platform was performed on cecal samples collected from these individuals at slaughter. Different univariate and multivariate approaches were conducted to unravel the influence of the different factors on microbial alpha diversity and composition at phylum, genus and OTU taxonomic levels. The animals raised in the facility harboring the most stable environmental conditions had greater, and less variable, microbial richness and diversity. Bootstrap univariate analyses of variance and sparse partial least squares-discriminant analyses endorsed that farm conditions exerted an important influence on rabbit microbiota since the relative abundances of many taxa were found differentially represented between both facilities at all taxonomic levels characterized. Furthermore, only five OTUs were needed to achieve a perfect classification of samples according to the facility where animals were raised. The level of feeding and the presence of antibiotics did not modify the global alpha diversity but had an impact on some bacteria relative abundances, albeit in a small number of taxa compared with farm, which is consistent with the lower sample classification power according to these factors achieved using microbial information. Conclusions This study reveals that factors associated with the farm effect and other management factors, such as the presence of antibiotics in the diet or the feeding level, modify cecal microbial communities. It highlights the importance of offering a controlled breeding environment that reduces differences in microbial cecal composition that could be responsible for different animal performance.

show abstract

“…The abundance of Thermoplasmatales , or some members of Thermoplasmatales might also be negatively correlated to certain Methanobrevibacter spp. ( Danielsson et al, 2017 ; McGovern et al, 2017 ). Methanobrevibacter are hydrogenotrophic methanogens ( Leahy et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Application of MootralTM Reduces Methane Production by Altering the Archaea Community in the Rumen Simulation Technique

Eger

Graz²,

Riede

et al. 2018

Front. Microbiol.

View full text Add to dashboard Cite

The reduction of methane emissions by ruminants is a highly desirable goal to mitigate greenhouse gas emissions. Various feed additives have already been tested for their ability to decrease methane production; however, practical use is often limited due to negative effects on rumen fermentation or high costs. Organosulphur compounds from garlic (Allium sativum) and flavonoids have been identified as promising plant-derived compounds which are able to reduce methane production. Here, we evaluated the effects of a combination of garlic powder and bitter orange (Citrus aurantium) extracts, Mootral, on ruminal methane production, ruminal fermentation and the community of methanogenic Archaea by using the rumen simulation technique as ex vivo model. The experiment consisted of an equilibration period of 7 days, an experimental period of 8 days and a withdrawal period of 4 days. During the experimental period three fermenters each were either treated as controls (CON), received a low dose of Mootral (LD), a high dose of Mootral (HD), or monensin (MON) as positive control. Application of Mootral strongly reduced the proportion of methane in the fermentation gas and the production rate of methane. Moreover, the experimental mixture induced a dose-dependent increase in the production rate of short chain fatty acids and in the molar proportion of butyrate. Some effects persisted during the withdrawal period. Both, single strand conformation polymorphism and Illumina MiSeq 16S rRNA amplicon sequencing indicated an archaeal community distinct from CON and MON samples in the LD and HD samples. Among archaeal families the percentage of Methanobacteriaceae was reduced during application of both doses of Mootral. Moreover, several significant differences were observed on OTU level among treatment groups and after withdrawal of the additives for LD and HD group. At day 14, 4 OTUs were positively correlated with methane production. In conclusion this mixture of garlic and citrus compounds appears to effectively reduce methane production by alteration of the archaeal community without exhibiting negative side effects on rumen fermentation.

show abstract

Plane of nutrition affects the phylogenetic diversity and relative abundance of transcriptionally active methanogens in the bovine rumen

Cited by 16 publications

References 26 publications

Comparative analysis of the metabolically active microbial communities in the rumen of dromedary camels under different feeding systems using total rRNA sequencing

Comparative analysis of the metabolically active microbial communities in the rumen of dromedary camels under different feeding systems using total rRNA sequencing

Breeding farm, level of feeding and presence of antibiotics in the feed influence rabbit cecal microbiota

Application of MootralTM Reduces Methane Production by Altering the Archaea Community in the Rumen Simulation Technique

Contact Info

Product

Resources

About