Planctomycetes diversity associated with macroalgae

Lage, Olga Maria; Bondoso, Joana

doi:10.1111/j.1574-6941.2011.01168.x

Cited by 120 publications

(147 citation statements)

References 71 publications

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“…Seaweeds and other marine algae contain an abundance of sulfated polysaccharides (77), two of which are fucoidan and ulvan, which are composed of 95% L-fucose (53) and 55% L-rhamnose (75), respectively. Members of the phylum Planctomycetes dominate seaweed-associated biofilms (78), frequently co-occur with algal blooms (79), and contain an abnormal abundance of sulfatase genes that are used to degrade sulfated polysaccharides (52); all of these observations are consistent with the prediction that many Planctomycetes species use a BMC in degrading algal polysaccharides.…”

Section: Discussionsupporting

confidence: 72%

Characterization of a Planctomycetal Organelle: a Novel Bacterial Microcompartment for the Aerobic Degradation of Plant Saccharides

Erbilgin

McDonald

Kerfeld

2014

Appl Environ Microbiol

123

142

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Bacterial microcompartments (BMCs) are organelles that encapsulate functionally linked enzymes within a proteinaceous shell. The prototypical example is the carboxysome, which functions in carbon fixation in cyanobacteria and some chemoautotrophs. It is increasingly apparent that diverse heterotrophic bacteria contain BMCs that are involved in catabolic reactions, and many of the BMCs are predicted to have novel functions. However, most of these putative organelles have not been experimentally characterized. In this study, we sought to discover the function of a conserved BMC gene cluster encoded in the majority of the sequenced planctomycete genomes. This BMC is especially notable for its relatively simple genetic composition, its remote phylogenetic position relative to characterized BMCs, and its apparent exclusivity to the enigmatic Verrucomicrobia and Planctomycetes. Members of the phylum Planctomycetes are known for their morphological dissimilarity to the rest of the bacterial domain: internal membranes, reproduction by budding, and lack of peptidoglycan. As a result, they are ripe for many discoveries, but currently the tools for genetic studies are very limited. We expanded the genetic toolbox for the planctomycetes and generated directed gene knockouts of BMC-related genes in Planctomyces limnophilus. A metabolic activity screen revealed that BMC gene products are involved in the degradation of a number of plant and algal cell wall sugars. Among these sugars, we confirmed that BMCs are formed and required for growth on L-fucose and L-rhamnose. Our results shed light on the functional diversity of BMCs as well as their ecological role in the planctomycetes, which are commonly associated with algae.

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Section: Discussionsupporting

confidence: 72%

Characterization of a Planctomycetal Organelle: a Novel Bacterial Microcompartment for the Aerobic Degradation of Plant Saccharides

Erbilgin

McDonald

Kerfeld

2014

Appl Environ Microbiol

123

142

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“…A similarity of 99.8-100 % exists among the members of cluster B. This putative new species has also been found in the epiphytic community of macroalgae from the north coast of Portugal (Lage and Bondoso 2011). Despite the differences in the geographic and environmental conditions, strain OJF1 is very similar to strain Rhodopirellula sp.…”

Section: Molecular Identification By 16s Rdna and Eric Fingerprintingmentioning

confidence: 96%

“…Identification of the isolates was done by 16S rRNA gene analysis after amplification with the primers 27f and 1492r (Lane 1991) by direct colony PCR as described by Lage and Bondoso (2011). Briefly, the PCR mixture consisted of 19 PCR buffer, 1.5 mM MgCl 2 , 1 unit of GoTaq Flexi DNA Polymerase, 200 lM of each deoxynucleoside triphosphate, 2 lM of each primer, and material from a single colony to a final volume of 50 ll.…”

Section: Molecular Identification Of the Isolatesmentioning

confidence: 99%

“…Despite the information gathered in the last years, the physiology and metabolism of Planctomycetes are still not well understood (Ward et al 2006), and an effort to improve the number of new cultivable strains is important. Improvements in the isolation methodology of Planctomycetes have been achieved (Schlesner 1994;Zengler et al 2002;Winkelmann and Harder 2009;Lage and Bondoso 2011).…”

Section: Introductionmentioning

confidence: 97%

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Isolation and characterization of Planctomycetes from the sediments of a fish farm wastewater treatment tank

2012

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The increasing ecological significance of Planctomycetes and the still limited knowledge of this group prompted us to obtain cultured isolates from the sediment of a treatment water recycling tank of a marine fish farm. Presence of strains from this group was assessed in the sediments and water column of the tank. Eleven isolates were obtained from the sediment sample by exploiting Planctomycetes natural resistance to several antibiotics and their capacity to degrade organic matter. Based on morphological characteristics and resistance to antibiotics, Planctomycetes were identified. Their phylogenetic affiliation was confirmed by the sequence analysis of the 16S rRNA gene that revealed the presence of a group of 6 isolates closely related to Rhodopirellula baltica and a cluster of 5 isolates with 97.7-97.9 % of similarity to this species, which probably are a different species of Rhodopirellula. ERIC-PCR profiles showed a higher discrimination within the two groups and allowed the identification of nine different genotypes within the isolated strains. This work corroborates the association of Rhodopirellula spp. with fish farm environments.

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“…The bacterial strain Pseudomonas putida NB3L (Lage and Bondoso, 2011) was grown in liquid M607 medium in the dark at 25 • C with shaking, until the exponential growth phase was reached, and then was diluted to 0.1 OD (750 nm). A mixture of penicillin (50 U/ml), streptomycin (50 µg/ml), and neomycin (100 µg/ml) was used as positive control.…”

Section: Bacterial Growth Inhibition Assaymentioning

confidence: 99%

Picocyanobacteria From a Clade of MarineCyanobiumRevealed Bioactive Potential Against Microalgae, Bacteria, and Marine Invertebrates

Costa

Ramos

et al. 2015

Journal of Toxicology and Environmental Health, Part A

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The production of bioactive compounds either toxic or with pharmacological applications by cyanobacteria is well established. However, picoplanktonic forms within this group of organisms have rarely been studied in this context. In this study, the toxicological potential of picocyanobacteria from a clade of marine Cyanobium strains isolated from the Portuguese coast was examined using different biological models. First, strains were identified by applying morphological and molecular approaches and cultured under lab conditions. A crude extract and three fractions reflecting a preliminary segregation of lipophilic metabolites were tested for toxicity with the marine microalga Nannochloropsis sp., the bacteria Pseudomonas sp., the brine shrimp Artemia salina, and fertilized eggs of the sea urchin Paracentrotus lividus. No significant apparent adverse effects were noted against Artemia salina. However, significant adverse effects were found in all other assays, with an inhibition of Nannochloropsis sp. and Pseudomonas sp. growth and marked reduction in Paracentrotus lividus larvae length. The results obtained indicated that Cyanobium genus may serve as a potential source of interesting bioactive compounds and emphasize the importance of also studying smaller picoplanktonic fractions of marine cyanobacteria.

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Planctomycetes diversity associated with macroalgae

Cited by 120 publications

References 71 publications

Characterization of a Planctomycetal Organelle: a Novel Bacterial Microcompartment for the Aerobic Degradation of Plant Saccharides

Characterization of a Planctomycetal Organelle: a Novel Bacterial Microcompartment for the Aerobic Degradation of Plant Saccharides

Isolation and characterization of Planctomycetes from the sediments of a fish farm wastewater treatment tank

Picocyanobacteria From a Clade of MarineCyanobiumRevealed Bioactive Potential Against Microalgae, Bacteria, and Marine Invertebrates

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