Planar organization of airway epithelial cell morphology using hydrogel grooves during ciliogenesis fails to induce ciliary alignment

Varma, Ratna; Poon, James; Liao, Zhongfa; Aitchison, J. S.; Waddell, Thomas K.; Karoubi, Golnaz; McGuigan, Alison P.

doi:10.1039/d1bm01327k

Cited by 2 publications

(3 citation statements)

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“…Previous studies on groove patterns mostly focused on synchronous changes of ditches/ridges, ,, where it is difficult to define the independent role of ditches/ridges in regulating stem cells. In this work, we investigated the effect and mechanism of microgrooves on the stem cell morphology and fibrosis differentiation by changing the width of ditches/ridges separately, to provide a theoretical basis for the surface modification of groove implants.…”

Section: Discussionmentioning

confidence: 99%

“…Ditch/Ridge Single-Parameter Changes. Most groove structures used in the literature have the same ridge and groove dimensions, 12,35,36 so it is confusing to define the independent effects of ditches/ridges on cell behaviors. Based on this problem, we developed a microgroove chip with a single-parameter change of ditch/ridge.…”

Section: Design and Fabrication Of Microgrooves Withmentioning

confidence: 99%

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Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force

Lei

Miao

Wang

et al. 2023

ACS Appl. Mater. Interfaces

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Groove patterns are widely used in material surface modifications. However, the independent role of ditches/ridges in regulating fibrosis of soft tissues is not well-understood, especially the lack of linkage evidence in vitro and in vivo. Herein, two kinds of combinational microgroove chips with the gradient ditch/ridge width were fabricated by photolithography technology, termed R and G groups, respectively. In group R, the ridge width was 1, 5, 10, and 30 μm, with a ditch width of 30 μm; in group G, the groove width was 5, 10, 20, and 30 μm, and the ridge width was 5 μm. The effect of microgrooves on the morphology, proliferation, and expression of fibrous markers of stem cells was systematically investigated in vitro. Moreover, thicknesses of fibrous capsules were evaluated after chips were implanted into the muscular pouches of rats for 5 months. The results show that microgrooves have almost no effect on cell proliferation but significantly modulate the morphology of cells and focal adhesions (FAs) in vitro, as well as fibrosis differentiation. In particular, the differentiation of stem cells is attenuated after the intracellular force caused by stress fibers and FAs is interfered by drugs, such as rotenone and blebbistatin. Histological analysis shows that patterns of high intracellular force can apparently stimulate soft tissue fibrosis in vivo. This study not only reveals the specific rules and mechanisms of ditch/ridge regulating stem cell behaviors but also offers insight into tailoring implant surface patterns to induce controlled soft tissue fibrosis.

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Section: Discussionmentioning

confidence: 99%

Section: Design and Fabrication Of Microgrooves Withmentioning

confidence: 99%

Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force

Lei

Miao

Wang

et al. 2023

ACS Appl. Mater. Interfaces

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“…One of the potential application areas of the SBDO paradigm is the engineering of functional lung and airway tissues, organoids, and even whole organs, with potential applications in screening and development of drug therapies [ 16 ], disease modeling [ 17 ] and, eventually, to create de novo tissues and organs for human transplantation [ 18 ]. A large body of work has focused on engineering airway tissues using synthetic [ 19 – 21 ] and donor-derived scaffolds [ 22 , 23 ]. Among the many scientific and translational challenges of this line of research, one of the most significant is the design and optimization of protocols and devices to sustain the relevant cells as they deposit and attach to suitable scaffolds, proliferate, migrate, and differentiate into the targeted cell types required for functional tissues.…”

Section: Introductionmentioning

confidence: 99%

In silico model development and optimization of in vitro lung cell population growth

Mostofinejad,

Romero,

Brinson

et al. 2024

PLoS ONE

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Tissue engineering predominantly relies on trial and error in vitro and ex vivo experiments to develop protocols and bioreactors to generate functional tissues. As an alternative, in silico methods have the potential to significantly reduce the timelines and costs of experimental programs for tissue engineering. In this paper, we propose a methodology to formulate, select, calibrate, and test mathematical models to predict cell population growth as a function of the biochemical environment and to design optimal experimental protocols for model inference of in silico model parameters. We systematically combine methods from the experimental design, mathematical statistics, and optimization literature to develop unique and explainable mathematical models for cell population dynamics. The proposed methodology is applied to the development of this first published model for a population of the airway-relevant bronchio-alveolar epithelial (BEAS-2B) cell line as a function of the concentration of metabolic-related biochemical substrates. The resulting model is a system of ordinary differential equations that predict the temporal dynamics of BEAS-2B cell populations as a function of the initial seeded cell population and the glucose, oxygen, and lactate concentrations in the growth media, using seven parameters rigorously inferred from optimally designed in vitro experiments.

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Planar organization of airway epithelial cell morphology using hydrogel grooves during ciliogenesis fails to induce ciliary alignment

Abstract: Topographical cues are known to influence cell organization both in native tissues and in vitro. In the trachea, the matrix beneath the epithelial lining is composed of collagen fibres that...

Cited by 2 publications

References 48 publications

Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force

Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force

In silico model development and optimization of in vitro lung cell population growth

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